PMID- 33203464 OWN - NLM STAT- MEDLINE DCOM- 20210614 LR - 20210614 IS - 1297-9716 (Electronic) IS - 0928-4249 (Print) IS - 0928-4249 (Linking) VI - 51 IP - 1 DP - 2020 Nov 17 TI - Glyceraldehyde-3-phosphate dehydrogenase from Eimeria acervulina modulates the functions of chicken dendritic cells to boost Th1 type immune response and stimulates autologous CD4(+) T cells differentiation in-vitro. PG - 138 LID - 10.1186/s13567-020-00864-z [doi] LID - 138 AB - Dendritic cells (DCs) play a pivotal role to amplify antigen-specific immune responses. Antigens that sensitize T cells via antigen-presentation by DCs could enhance the capacity of host immunity to fight infections. In this study, we tested the immunogenic profiles of chicken DCs towards Glyceraldehyde-3-phosphate dehydrogenase from Eimeria acervulina (EaGAPDH). Immunoblot analysis showed that recombinant EaGAPDH (rEaGAPDH) protein was successfully recognized by rat sera generated against rEaGAPDH. Interaction and internalisation of rEaGAPDH by chicken splenic-derived DCs (chSPDCs) was confirmed by immunofluorescence analysis. Flow cytometry revealed that chSPDCs upregulated MHCII, CD1.1, CD11c, CD80, and CD86 cell-surface markers. Moreover, mRNA expressions of DC maturation biomarkers (CCL5, CCR7, and CD83) and TLR signalling genes (TLR15 and MyD88) were also upregulated whereas those of Wnt signalling were non-significant compared to negative controls. rEaGAPDH treatment induced IL-12 and IFN-gamma secretion in chSPDCs but had no effect on IL-10 and TGF-beta. Likewise, DC-T cell co-culture promoted IFN-gamma secretion and the level of IL-4 was unaffected. Proliferation of T cells and their differentiation into CD3(+)/CD4(+) T cells were triggered in chSPDCs-T cells co-culture system. Taken together, rEaGAPDH could promote Th1 polarization by activating both host DCs and T cells and sheds new light on the role of this important molecule which might contribute to the development of new DCs-based immunotherapeutic strategies against coccidiosis. FAU - Lakho, Shakeel Ahmed AU - Lakho SA AD - MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China. FAU - Haseeb, Muhammad AU - Haseeb M AD - MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China. FAU - Huang, Jianmei AU - Huang J AD - MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China. FAU - Yang, Zhang AU - Yang Z AD - MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China. FAU - Hasan, Muhammad Waqqas AU - Hasan MW AD - MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China. FAU - Aleem, Muhammad Tahir AU - Aleem MT AD - MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China. FAU - Naqvi, Muhammad Ali-Ul-Husnain AU - Naqvi MA AD - MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China. FAU - Memon, Muhammad Ali AU - Memon MA AD - MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China. FAU - Song, XiaoKai AU - Song X AD - MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China. FAU - Yan, RuoFeng AU - Yan R AD - MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China. FAU - Xu, Lixin AU - Xu L AD - MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China. FAU - Li, XiangRui AU - Li X AUID- ORCID: 0000-0003-3538-8837 AD - MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China. lixiangrui@njau.edu.cn. LA - eng GR - Grant No. 31661143017/Joint Research Project between the National Natural Science Foundation China and Pakistan Science Foundation/ PT - Journal Article DEP - 20201117 PL - England TA - Vet Res JT - Veterinary research JID - 9309551 RN - 0 (Protozoan Proteins) RN - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases) SB - IM MH - Animals MH - CD4-Positive T-Lymphocytes/*immunology MH - Cell Differentiation MH - Chickens/*immunology MH - Coccidiosis/immunology/veterinary MH - Dendritic Cells/*immunology MH - Eimeria/enzymology/*physiology MH - Glyceraldehyde-3-Phosphate Dehydrogenases MH - Immunity/*genetics MH - Poultry Diseases/immunology MH - Protozoan Proteins/*metabolism MH - Th1 Cells/*immunology PMC - PMC7672913 OTO - NOTNLM OT - CD4+ T cells OT - Eimeria species OT - chicken dendritic cells OT - coccidiosis OT - cytokines OT - glyceraldehyde-3-phosphate dehydrogenase OT - toll-like receptors COIS- The authors declare that they have no competing interests. EDAT- 2020/11/19 06:00 MHDA- 2021/06/16 06:00 PMCR- 2020/11/17 CRDT- 2020/11/18 05:35 PHST- 2020/07/26 00:00 [received] PHST- 2020/10/26 00:00 [accepted] PHST- 2020/11/18 05:35 [entrez] PHST- 2020/11/19 06:00 [pubmed] PHST- 2021/06/16 06:00 [medline] PHST- 2020/11/17 00:00 [pmc-release] AID - 10.1186/s13567-020-00864-z [pii] AID - 864 [pii] AID - 10.1186/s13567-020-00864-z [doi] PST - epublish SO - Vet Res. 2020 Nov 17;51(1):138. doi: 10.1186/s13567-020-00864-z.