PMID- 33203899
OWN - NLM
STAT- MEDLINE
DCOM- 20210114
LR  - 20240331
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Nov 17
TI  - Epstein-Barr virus peptides derived from latent cycle proteins alter NKG2A + NK 
      cell effector function.
PG  - 19973
LID - 10.1038/s41598-020-76344-3 [doi]
LID - 19973
AB  - Natural killer (NK) cells control viral infection through the interaction between 
      inhibitory receptors and human leukocyte antigen (HLA) ligands and bound peptide. 
      NK cells expressing the inhibitory receptor NKG2A/CD94 recognize and respond to 
      autologous B cells latently infected with Epstein-Barr virus (EBV). The mechanism 
      is not yet understood, thus we investigated peptides derived from seven latent 
      proteins of EBV in the interaction of NKG2A and its ligand HLA-E. Functional 
      analysis demonstrated that EBV peptides can bind to HLA-E and block inhibition of 
      NK cell effector function. Moreover, analysis of DNA from 79 subjects showed 
      sequence variations in the latent protein, LMP1, which alters NK responses to 
      EBV. We provide evidence that peptides derived from EBV latent cycle proteins can 
      impair the recognition of NKG2A despite being presented by HLA-E, resulting in NK 
      cell activation.
FAU - Mbiribindi, Berenice
AU  - Mbiribindi B
AD  - Division of Abdominal Transplantation, Department of Surgery, Stanford University 
      School of Medicine, Stanford, CA, USA.
FAU - Pena, Josselyn K
AU  - Pena JK
AD  - Division of Abdominal Transplantation, Department of Surgery, Stanford University 
      School of Medicine, Stanford, CA, USA.
FAU - Arvedson, Matthew P
AU  - Arvedson MP
AD  - Division of Abdominal Transplantation, Department of Surgery, Stanford University 
      School of Medicine, Stanford, CA, USA.
FAU - Moreno Romero, Claudia
AU  - Moreno Romero C
AD  - Division of Abdominal Transplantation, Department of Surgery, Stanford University 
      School of Medicine, Stanford, CA, USA.
FAU - McCarthy, Sarah R
AU  - McCarthy SR
AD  - Division of Abdominal Transplantation, Department of Surgery, Stanford University 
      School of Medicine, Stanford, CA, USA.
FAU - Hatton, Olivia L
AU  - Hatton OL
AD  - Department of Molecular Biology, Colorado College, Colorado Springs, CO, USA.
FAU - Esquivel, Carlos O
AU  - Esquivel CO
AD  - Division of Abdominal Transplantation, Department of Surgery, Stanford University 
      School of Medicine, Stanford, CA, USA.
FAU - Martinez, Olivia M
AU  - Martinez OM
AD  - Division of Abdominal Transplantation, Department of Surgery, Stanford University 
      School of Medicine, Stanford, CA, USA.
FAU - Krams, Sheri M
AU  - Krams SM
AD  - Division of Abdominal Transplantation, Department of Surgery, Stanford University 
      School of Medicine, Stanford, CA, USA. smkrams@stanford.edu.
LA  - eng
GR  - U01 AI135950/AI/NIAID NIH HHS/United States
GR  - T32 AI007290/AI/NIAID NIH HHS/United States
GR  - U01 AI135947/AI/NIAID NIH HHS/United States
GR  - S10 RR027431/RR/NCRR NIH HHS/United States
GR  - U01 AI104342/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20201117
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (KLRC1 protein, human)
RN  - 0 (Ligands)
RN  - 0 (NK Cell Lectin-Like Receptor Subfamily C)
RN  - 0 (Peptides)
SB  - IM
MH  - B-Lymphocytes/metabolism
MH  - Cell Line
MH  - Herpesvirus 4, Human/*metabolism
MH  - Histocompatibility Antigens Class I/metabolism
MH  - Humans
MH  - Killer Cells, Natural/*metabolism
MH  - Latent Infection/*metabolism
MH  - Ligands
MH  - NK Cell Lectin-Like Receptor Subfamily C/*metabolism
MH  - Peptides/metabolism
PMC - PMC7673117
COIS- The authors declare no competing interests.
EDAT- 2020/11/19 06:00
MHDA- 2021/01/15 06:00
PMCR- 2020/11/17
CRDT- 2020/11/18 05:53
PHST- 2020/09/01 00:00 [received]
PHST- 2020/10/21 00:00 [accepted]
PHST- 2020/11/18 05:53 [entrez]
PHST- 2020/11/19 06:00 [pubmed]
PHST- 2021/01/15 06:00 [medline]
PHST- 2020/11/17 00:00 [pmc-release]
AID - 10.1038/s41598-020-76344-3 [pii]
AID - 76344 [pii]
AID - 10.1038/s41598-020-76344-3 [doi]
PST - epublish
SO  - Sci Rep. 2020 Nov 17;10(1):19973. doi: 10.1038/s41598-020-76344-3.