PMID- 33204526
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201119
IS  - 2090-8016 (Print)
IS  - 2090-0597 (Electronic)
IS  - 2090-0597 (Linking)
VI  - 2020
DP  - 2020
TI  - Rejuvenated Circulating Endothelial Progenitor Cells and Nitric Oxide in 
      Premenopausal Women with Hyperhomocysteinemia.
PG  - 5010243
LID - 10.1155/2020/5010243 [doi]
LID - 5010243
AB  - Hyperhomocysteinemia (HHcy) induced endothelial dysfunction is associated with 
      disturbance in circulating endothelial progenitor cells (EPCs). Nevertheless, 
      whether this unfavorable effect of HHcy on circulating EPCs also exists in 
      premenopausal women is still unknown. Therefore, this leaves an area for the 
      investigation of the difference on the number and activity of circulating EPCs in 
      premenopausal women with hyperhomocysteinemia and its underlying mechanism. The 
      number of circulating EPCs was measured by fluorescence-activated cell sorter 
      analysis, as well as DiI-acLDL and lectin fluorescent staining. The migration and 
      proliferation of circulating were evaluated by the Transwell chamber assay and 
      MTT. Additionally, the endothelial function and levels of nitric oxide (NO), 
      VEGF, and GM-CSF in plasma and culture medium were determined. The number or 
      activity of circulating EPCs and flow-mediated dilatation (FMD) in premenopausal 
      women with or without HHcy were higher than those in postmenopausal women. 
      However, no significant effect of HHcy on the number or activity of circulating 
      EPCs in premenopausal women was observed. A similar alteration in NO level 
      between the four groups was observed. There was a correlation between FMD and the 
      number or activity of EPCs, as well as NO level in plasma or secretion by EPCs. 
      For the first time, our findings illuminated the quantitive or qualitative 
      alterations of circulating EPCs and endothelial function in premenopausal 
      patients with HHcy are preserved, which was associated with retained NO 
      production. The recuperated endothelial repair capacity is possibly the potential 
      mechanism interpreting cardiovascular protection in premenopausal women with 
      HHcy.
CI  - Copyright (c) 2020 Long Peng et al.
FAU - Peng, Long
AU  - Peng L
AD  - Department of Cardiovascular Medicine, The Third Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou 510630, China.
FAU - Gu, Qianlin
AU  - Gu Q
AD  - The Division of Emergency Medicine, The First Affiliated Hospital of Sun Yat-sen 
      University, No. 58, Zhongshan 2th Road, Yuexiu District, Guangzhou 510080, China.
FAU - Huang, Zhenhua
AU  - Huang Z
AD  - The Division of Emergency Medicine, The First Affiliated Hospital of Sun Yat-sen 
      University, No. 58, Zhongshan 2th Road, Yuexiu District, Guangzhou 510080, China.
FAU - Zeng, Lijin
AU  - Zeng L
AD  - The Division of Emergency Medicine, The First Affiliated Hospital of Sun Yat-sen 
      University, No. 58, Zhongshan 2th Road, Yuexiu District, Guangzhou 510080, China.
FAU - Chu, Chang
AU  - Chu C
AUID- ORCID: 0000-0002-6842-2897
AD  - Department of Cardiovascular Medicine and Dongguan Cardiovascular Institute, The 
      Third People's Hospital of Dongguan City, Dongguan 523326, China.
FAU - Yang, Xiaoan
AU  - Yang X
AUID- ORCID: 0000-0001-5520-2919
AD  - Department of Infectious Diseases, The 3rd Affiliated Hospital, Sun Yat-Sen 
      University, No. 600, Tianhe Road, Tianhe District, Guangzhou 510630, China.
LA  - eng
PT  - Journal Article
DEP - 20201031
PL  - United States
TA  - Cardiol Res Pract
JT  - Cardiology research and practice
JID - 101516542
PMC - PMC7657675
COIS- The authors declare that there are no conflicts of interest regarding the 
      publication of this paper.
EDAT- 2020/11/19 06:00
MHDA- 2020/11/19 06:01
PMCR- 2020/10/31
CRDT- 2020/11/18 05:59
PHST- 2020/02/05 00:00 [received]
PHST- 2020/09/08 00:00 [revised]
PHST- 2020/09/24 00:00 [accepted]
PHST- 2020/11/18 05:59 [entrez]
PHST- 2020/11/19 06:00 [pubmed]
PHST- 2020/11/19 06:01 [medline]
PHST- 2020/10/31 00:00 [pmc-release]
AID - 10.1155/2020/5010243 [doi]
PST - epublish
SO  - Cardiol Res Pract. 2020 Oct 31;2020:5010243. doi: 10.1155/2020/5010243. 
      eCollection 2020.