PMID- 33206750
OWN - NLM
STAT- MEDLINE
DCOM- 20210426
LR  - 20220629
IS  - 1980-5322 (Electronic)
IS  - 1807-5932 (Print)
IS  - 1807-5932 (Linking)
VI  - 75
DP  - 2020
TI  - Inhibition of lipopolysaccharide-induced inflammation via the protective role of 
      T regulatory cells in the fetal liver in a late-pregnancy preterm mouse model.
PG  - e1665
LID - 10.6061/clinics/2020/e1665 [doi]
LID - e1665
AB  - OBJECTIVES: This study intended to explore the effect of T regulatory cells 
      (Tregs) in the perinatal liver against LPS-induced inflammation in a preterm 
      birth mouse model. Moreover, the role of adoptive Tregs on the inflammatory 
      response induced by LPS was also studied. METHODS: Female BALB/C mice were 
      injected intraperitoneally (IP) with LPS dissolved in normal saline solution at a 
      dose of 50 microg/kg. Spleens from pregnant mice were used to obtain Tregs. The 
      expression of Forkhead family transcription factor-3 (Foxp3), Interleukin-6 
      (IL-6), Toll-like receptor-4 (TLR-4), and Heme oxygenase-1 (HO-1) were assessed 
      from fetal liver tissues by polymerase chain reaction and western blotting. 
      RESULTS: LPS administered to mice induced an inflammatory response in the 
      perinatal liver, and this inflammatory response was negatively regulated by Tregs 
      in the experimental group. Maternal-fetal tolerance was maintained by Tregs. 
      Transmission of Tregs was estimated in different experimental groups based on the 
      mRNA expression of TLR-4, IL-6, HO-1, and Foxp3. CONCLUSIONS: After analysis of 
      the experimental data, it was determined that Tregs exhibited regulatory 
      potential against LPS-induced inflammatory response. Further, it was concluded 
      that the transmission of Tregs improved the mother's immune tolerance against 
      LPS-induced inflammation in the fetal liver.
FAU - Siddiq, Muhammad
AU  - Siddiq M
AUID- ORCID: 0000-0002-9891-9837
AD  - Department of Neonatology, The First Affiliated Hospital of Xi'an Jiaotong 
      University College of Medicine, Xi'an, Shaanxi, China.
FAU - Wang, Fan
AU  - Wang F
AUID- ORCID: 0000-0001-7374-3567
AD  - Department of Neonatology, The First Affiliated Hospital of Xi'an Jiaotong 
      University College of Medicine, Xi'an, Shaanxi, China.
FAU - Xiao, Mi
AU  - Xiao M
AUID- ORCID: 0000-0001-6474-2458
AD  - Department of Neonatology, The First Affiliated Hospital of Xi'an Jiaotong 
      University College of Medicine, Xi'an, Shaanxi, China.
FAU - Lin, Xiao Jie
AU  - Lin XJ
AUID- ORCID: 0000-0001-6730-9342
AD  - Department of Neonatology, The First Affiliated Hospital of Xi'an Jiaotong 
      University College of Medicine, Xi'an, Shaanxi, China.
FAU - Fatima, Nazira
AU  - Fatima N
AUID- ORCID: 0000-0002-7571-0440
AD  - Department of Biotechnology, Mirpur University of Science & Technology Pakistan, 
      Pakistan and Laboratory Animal Center Xian Jiaotong University Health Science 
      Centre, Xian Shaanxi, China.
FAU - Iqbal, Sara
AU  - Iqbal S
AUID- ORCID: 0000-0003-4027-1190
AD  - Akhtar Saeed Medical & Dental College, Akhtar Saeed Trust Hospital & Farooq 
      Hospital Lahore, Akhtar Saeed Medical & Dental CollegeAkhtar Saeed Trust Hospital 
      & Farooq Hospital LahorePakistanPakistan.
FAU - Iqbal, Umar
AU  - Iqbal U
AUID- ORCID: 0000-0003-2802-9238
AD  - Akhtar Saeed Medical & Dental College, Akhtar Saeed Trust Hospital & Farooq 
      Hospital Lahore, Akhtar Saeed Medical & Dental CollegeAkhtar Saeed Trust Hospital 
      & Farooq Hospital LahorePakistanPakistan.
FAU - Piao, Xian-Hua
AU  - Piao XH
AUID- ORCID: 0000-0003-0412-517X
AD  - The Division of Newborn Medicine, Boston Children's Hospital of Harvard Medical 
      School, Boston, Massachusetts, U.S.A.
FAU - Liu, Li
AU  - Liu L
AUID- ORCID: 0000-0003-1200-3068
AD  - Department of Neonatology, The First Affiliated Hospital of Xi'an Jiaotong 
      University College of Medicine, Xi'an, Shaanxi, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201111
PL  - United States
TA  - Clinics (Sao Paulo)
JT  - Clinics (Sao Paulo, Brazil)
JID - 101244734
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Animals
MH  - Female
MH  - Forkhead Transcription Factors
MH  - Inflammation/chemically induced
MH  - *Lipopolysaccharides/toxicity
MH  - Liver
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Pregnancy
MH  - *Premature Birth
MH  - T-Lymphocytes, Regulatory
PMC - PMC7603294
COIS- No potential conflict of interest was reported.
EDAT- 2020/11/19 06:00
MHDA- 2021/04/27 06:00
PMCR- 2020/01/01
CRDT- 2020/11/18 17:10
PHST- 2019/12/27 00:00 [received]
PHST- 2020/03/26 00:00 [accepted]
PHST- 2020/11/18 17:10 [entrez]
PHST- 2020/11/19 06:00 [pubmed]
PHST- 2021/04/27 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - S1807-5932(22)00330-1 [pii]
AID - cln_75p1 [pii]
AID - 10.6061/clinics/2020/e1665 [doi]
PST - epublish
SO  - Clinics (Sao Paulo). 2020 Nov 11;75:e1665. doi: 10.6061/clinics/2020/e1665. 
      eCollection 2020.