PMID- 33210785
OWN - NLM
STAT- MEDLINE
DCOM- 20210625
LR  - 20210625
IS  - 1365-313X (Electronic)
IS  - 0960-7412 (Linking)
VI  - 105
IP  - 4
DP  - 2021 Feb
TI  - An efficient Oligo-FISH painting system for revealing chromosome rearrangements 
      and polyploidization in Triticeae.
PG  - 978-993
LID - 10.1111/tpj.15081 [doi]
AB  - A chromosome-specific painting technique has been developed which combines the 
      most recent approaches of the companion disciplines of molecular cytogenetics and 
      genome research. We developed seven oligonucleotide (oligo) pools derivd from 
      single-copy sequences on chromosomes 1 to 7 of barley (Hordeum vulgare L.) and 
      corresponding collinear regions of wheat (Triticum aestivum L.). The seven groups 
      of pooled oligos comprised between 10 986 and 12 496 45-bp monomers, and these 
      then produced stable fluorescence in situ hybridization (FISH) signals on 
      chromosomes of each linkage group of wheat and barley. The pooled oligo probes 
      were applied to high-throughput karyotyping of the chromosomes of other Triticeae 
      species in the genera Secale, Aegilops, Thinopyrum, and Dasypyrum, and the study 
      also extended to some wheat-alien amphiploids and derived lines. We demonstrated 
      that a complete set of whole-chromosome oligo painting probes facilitated the 
      study of inter-species chromosome homologous relationships and visualized 
      non-homologous chromosomal rearrangements in Triticeae species and some 
      wheat-alien species derivatives. When combined with other non-denaturing FISH 
      procedures using tandem-repeat oligos, the newly developed oligo painting 
      techniques provide an efficient tool for the study of chromosome structure, 
      organization, and evolution among any wild Triticeae species with non-sequenced 
      genomes.
CI  - (c) 2020 Society for Experimental Biology and John Wiley & Sons Ltd.
FAU - Li, Guangrong
AU  - Li G
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic and Technology of China, Chengdu, 611731, China.
FAU - Zhang, Tao
AU  - Zhang T
AD  - Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of 
      Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern 
      Production Technology of Grain Crops, Agricultural College of Yangzhou 
      University, Yangzhou, 225009, China.
AD  - Key Laboratory of Plant Functional Genomics of the Ministry of Education/Joint 
      International Research Laboratory of Agriculture and Agri-Product Safety of 
      Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China.
FAU - Yu, Zhihui
AU  - Yu Z
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic and Technology of China, Chengdu, 611731, China.
FAU - Wang, Hongjin
AU  - Wang H
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic and Technology of China, Chengdu, 611731, China.
FAU - Yang, Ennian
AU  - Yang E
AD  - Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 
      610066, China.
FAU - Yang, Zujun
AU  - Yang Z
AUID- ORCID: 0000-0003-4617-1422
AD  - Center for Informational Biology, School of Life Science and Technology, 
      University of Electronic and Technology of China, Chengdu, 611731, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201213
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Oligonucleotides)
SB  - IM
MH  - Aegilops/genetics
MH  - Chromosome Painting/*methods
MH  - Chromosomes, Plant/*genetics
MH  - Gene Library
MH  - Gene Rearrangement/*genetics
MH  - Genetic Linkage/genetics
MH  - Hordeum/*genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Oligonucleotides/genetics
MH  - Poaceae/*genetics
MH  - Secale/genetics
MH  - Translocation, Genetic/genetics
MH  - Triticum/*genetics
OTO - NOTNLM
OT  - Oligo-FISH
OT  - Triticeae species
OT  - chromosome rearrangement
OT  - wheat-barley synteny
EDAT- 2020/11/20 06:00
MHDA- 2021/06/29 06:00
CRDT- 2020/11/19 08:48
PHST- 2020/09/03 00:00 [received]
PHST- 2020/10/25 00:00 [revised]
PHST- 2020/11/09 00:00 [accepted]
PHST- 2020/11/20 06:00 [pubmed]
PHST- 2021/06/29 06:00 [medline]
PHST- 2020/11/19 08:48 [entrez]
AID - 10.1111/tpj.15081 [doi]
PST - ppublish
SO  - Plant J. 2021 Feb;105(4):978-993. doi: 10.1111/tpj.15081. Epub 2020 Dec 13.