PMID- 33210990
OWN - NLM
STAT- MEDLINE
DCOM- 20210714
LR  - 20240403
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 102
IP  - 2
DP  - 2021 Feb
TI  - Alfalfa leaf curl virus is efficiently acquired by its aphid vector Aphis 
      craccivora but inefficiently transmitted.
LID - 10.1099/jgv.0.001516 [doi]
LID - 001516
AB  - Alfalfa leaf curl virus (ALCV) is the first geminivirus for which aphid 
      transmission was reported. Transmission by Aphis craccivora was determined 
      previously to be highly specific and circulative. Using various complementary 
      techniques, the transmission journey of ALCV was monitored from its uptake from 
      infected plant tissues up to the head of its vector. ALCV was shown to be 
      restricted to phloem tissues using fluorescence in situ hybridization (FISH) and 
      electropenetrography (EPG) monitoring of virus acquisition. Furthermore, the 
      virus is heterogeneously distributed in phloem tissues, as revealed by FISH and 
      quantitative PCR of viral DNA acquired by EPG-monitored aphids. Despite the 
      efficient ingestion of viral DNA, about 10(6) viral DNA copies per insect in a 
      15 h feeding period on ALCV-infected plants, the individual maximum transmission 
      rate was 12 %. Transmission success was related to a critical viral accumulation, 
      around 1.6x10(7) viral DNA copies per insect, a threshold that generally needed 
      more than 48 h to be reached. Moreover, whereas the amount of acquired virus did 
      not decrease over time in the whole aphid body, it declined in the haemolymph and 
      heads. ALCV was not detected in progenies of viruliferous aphids and did not 
      affect aphid fitness. Compared to geminiviruses transmitted by whiteflies or 
      leafhoppers, or to luteoviruses transmitted by aphids, the transmission 
      efficiency of ALCV by A. craccivora is low. This result is discussed in relation 
      to the aphid vector of this geminivirus and the agroecological features of 
      alfalfa, a hardy perennial host plant.
FAU - Ryckebusch, Faustine
AU  - Ryckebusch F
AD  - CIRAD, UMR BGPI, Montpellier, France.
AD  - BGPI, Univ Montpellier, INRAE, CIRAD, Montpellier SupAgro, Montpellier, France.
AD  - Global Health Institute, School of Life Science, Ecole Polytechnique Federale de 
      Lausanne (EPFL), Lausanne, Switzerland.
FAU - Peterschmitt, Michel
AU  - Peterschmitt M
AD  - CIRAD, UMR BGPI, Montpellier, France.
AD  - BGPI, Univ Montpellier, INRAE, CIRAD, Montpellier SupAgro, Montpellier, France.
FAU - Granier, Martine
AU  - Granier M
AD  - CIRAD, UMR BGPI, Montpellier, France.
AD  - BGPI, Univ Montpellier, INRAE, CIRAD, Montpellier SupAgro, Montpellier, France.
FAU - Sauvion, Nicolas
AU  - Sauvion N
AD  - BGPI, Univ Montpellier, INRAE, CIRAD, Montpellier SupAgro, Montpellier, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Aphids/physiology/*virology
MH  - DNA, Viral/genetics
MH  - Geminiviridae/classification/genetics/*physiology
MH  - In Situ Hybridization, Fluorescence
MH  - Insect Vectors/*virology
MH  - Medicago sativa/*virology
MH  - Plant Diseases/*virology
PMC - PMC8116941
OTO - NOTNLM
OT  - Aphididae
OT  - Capulavirus
OT  - Hemiptera
OT  - epidemiology
OT  - luteovirus
OT  - nanovirus
COIS- The authors declare that there are no conflicts of interest.
EDAT- 2020/11/20 06:00
MHDA- 2021/07/15 06:00
PMCR- 2020/11/19
CRDT- 2020/11/19 12:13
PHST- 2020/11/20 06:00 [pubmed]
PHST- 2021/07/15 06:00 [medline]
PHST- 2020/11/19 12:13 [entrez]
PHST- 2020/11/19 00:00 [pmc-release]
AID - 001516 [pii]
AID - 10.1099/jgv.0.001516 [doi]
PST - ppublish
SO  - J Gen Virol. 2021 Feb;102(2):001516. doi: 10.1099/jgv.0.001516.