PMID- 33213426
OWN - NLM
STAT- MEDLINE
DCOM- 20210720
LR  - 20240330
IS  - 2662-7671 (Electronic)
IS  - 2662-7671 (Linking)
VI  - 20
IP  - 1
DP  - 2020 Nov 19
TI  - Evaluation of immunomodulatory effects of Boswellia sacra essential oil on 
      T-cells and dendritic cells.
PG  - 352
LID - 10.1186/s12906-020-03146-5 [doi]
LID - 352
AB  - BACKGROUND: Boswellia sacra resin has been commonly used as analgesic, 
      antimicrobial, and anti-inflammatory properties, which reflect its 
      immunomodulatory activity. Dendritic cells (DCs) are specialized 
      antigen-presenting cells (APCs) and sentinel cells that regulate the immune 
      response. This study aims at investigating whether crude essential oil extracted 
      from Boswellia sacra resin (BSEO), has a potential effect on the phenotype and 
      functions of human monocyte-derived DCs. METHODS: Oil extract from the resin of 
      Boswellia sacra was prepared by hydrodistillation using a custom made 
      hydrodistiller. BSEO-mediated cell viability has been initially studied on human 
      skin dermis cells (HSD) and DC precursors using quantitative and qualitative 
      assays before applying on DCs. Human DCs were generated from differentiated 
      peripheral blood monocytes cultured in media containing both GM-CSF and IL-4. DCs 
      were exposed to 5 mug/mL or 10 mug/mL of BSEO in vitro. Morphological, 
      phonotypical, and functional properties studied with microscopy, flow cytometry, 
      and ELISA. RESULTS: Crude BSEO was found to interfere with the maturation and 
      differentiation of DCs from precursor cells in the presence or absence of 
      lipopolysaccharide (LPS). BSEO-treated DCs, cultured in the presence of LPS, 
      reduced the ability of allogeneic T cells to proliferate compared to that 
      co-cultured with LPS-stimulated DCs only. In addition, the endocytic capacity and 
      secretion of IL-10 by DCs treated with BSEO was enhanced in comparison to LPS 
      treated cells. Analysis of the chemical composition of BESO using GC-MS (Clarus 
      500 GC/MS, PerkinElmer, Shelton, CT) revealed the presence of compounds with 
      several biological activities including antibacterial, antioxidant, and 
      anti-inflammatory properties. CONCLUSION: Results indicated that BSEO deviates 
      the differentiation of monocytes into immature DCs. Furthermore, stimulation of 
      immature DCs with BSEO was unable to generate full DC maturation. However, these 
      findings may potentially be employed to generate DCs with tolerogenic properties 
      that are able to induce tolerance in diseases with hypersensitivity, autoimmunity 
      as well as transplantation.
FAU - Aldahlawi, Alia M
AU  - Aldahlawi AM
AUID- ORCID: 0000-0001-9599-050X
AD  - Department of Biological Sciences, Faculty of Sciences, King Abdulaziz 
      University, Jeddah, Saudi Arabia. aaldahlawi@kau.edu.sa.
AD  - Immunology Unit, King Fahad Medical Research Center, King Abdulaziz University, 
      Jeddah, Saudi Arabia. aaldahlawi@kau.edu.sa.
FAU - Alzahrani, Amani T
AU  - Alzahrani AT
AD  - Department of Biological Sciences, Faculty of Sciences, King Abdulaziz 
      University, Jeddah, Saudi Arabia.
FAU - Elshal, Mohamed F
AU  - Elshal MF
AD  - Molecular Biology Department, Genetic Engineering and Biotechnology Institute, 
      University of Sadat City, Sadat City, Egypt. Mohamed.elshal@gebri.usc.edu.eg.
LA  - eng
GR  - PS-35-190/King Abdulaziz City for Science and Technology/
PT  - Journal Article
DEP - 20201119
PL  - England
TA  - BMC Complement Med Ther
JT  - BMC complementary medicine and therapies
JID - 101761232
RN  - 0 (Cytokines)
RN  - 0 (Oils, Volatile)
SB  - IM
MH  - Apoptosis/drug effects
MH  - *Boswellia
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cytokines/drug effects
MH  - Dendritic Cells/*drug effects
MH  - Humans
MH  - Immunomodulation/*drug effects
MH  - Monocytes/drug effects
MH  - Oils, Volatile/*pharmacology
MH  - T-Lymphocytes/*drug effects
PMC - PMC7678202
OTO - NOTNLM
OT  - And tolerance
OT  - Boswellia sacra
OT  - Dendritic cells
OT  - Differentiation
OT  - Essential oil
OT  - Maturation
COIS- The authors declare that they have no competing interests in this section.
EDAT- 2020/11/21 06:00
MHDA- 2021/07/21 06:00
PMCR- 2020/11/19
CRDT- 2020/11/20 05:29
PHST- 2020/08/10 00:00 [received]
PHST- 2020/11/02 00:00 [accepted]
PHST- 2020/11/20 05:29 [entrez]
PHST- 2020/11/21 06:00 [pubmed]
PHST- 2021/07/21 06:00 [medline]
PHST- 2020/11/19 00:00 [pmc-release]
AID - 10.1186/s12906-020-03146-5 [pii]
AID - 3146 [pii]
AID - 10.1186/s12906-020-03146-5 [doi]
PST - epublish
SO  - BMC Complement Med Ther. 2020 Nov 19;20(1):352. doi: 10.1186/s12906-020-03146-5.