PMID- 33213475
OWN - NLM
STAT- MEDLINE
DCOM- 20210603
LR  - 20210603
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 16
IP  - 1
DP  - 2020 Nov 19
TI  - Identification of an IRF10 gene in common carp (Cyprinus carpio L.) and analysis 
      of its function in the antiviral and antibacterial immune response.
PG  - 450
LID - 10.1186/s12917-020-02674-z [doi]
LID - 450
AB  - BACKGROUND: Interferon (IFN) regulatory factors (IRFs), as transcriptional 
      regulatory factors, play important roles in regulating the expression of type I 
      IFN and IFN- stimulated genes (ISGs) in innate immune responses. In addition, 
      they participate in cell growth and development and regulate oncogenesis. 
      RESULTS: In the present study, the cDNA sequence of IRF10 in common carp 
      (Cyprinus carpio L.) was characterized (abbreviation, CcIRF10). The predicted 
      protein sequence of CcIRF10 shared 52.7-89.2% identity with other teleost IRF10s 
      and contained a DNA-binding domain (DBD), a nuclear localization signal (NLS) and 
      an IRF-associated domain (IAD). Phylogenetic analysis showed that CcIRF10 had the 
      closest relationship with IRF10 of Ctenopharyngodon idella. CcIRF10 transcripts 
      were detectable in all examined tissues, with the highest expression in the gonad 
      and the lowest expression in the head kidney. CcIRF10 expression was upregulated 
      in the spleen, head kidney, foregut and hindgut upon polyinosinic:polycytidylic 
      acid (poly I:C) and Aeromonas hydrophila stimulation and induced by poly I:C, 
      lipopolysaccharide (LPS) and peptidoglycan (PGN) in peripheral blood leucocytes 
      (PBLs) and head kidney leukocytes (HKLs) of C. carpio. In addition, 
      overexpression of CcIRF10 was able to decrease the expression of the IFN and 
      IFN-stimulated genes PKR and ISG15. CONCLUSIONS: These results indicate that 
      CcIRF10 participates in antiviral and antibacterial immunity and negatively 
      regulates the IFN response, which provides new insights into the IFN system of C. 
      carpio.
FAU - Zhu, Yaoyao
AU  - Zhu Y
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan, 250014, 
      China.
AD  - College of Fisheries and Life Science, Hainan Tropical Ocean University, No. 1 
      Yucai Road, Sanya, 572022, China.
FAU - Shan, Shijuan
AU  - Shan S
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan, 250014, 
      China.
FAU - Zhao, Huaping
AU  - Zhao H
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan, 250014, 
      China.
FAU - Liu, Rongrong
AU  - Liu R
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan, 250014, 
      China.
FAU - Wang, Hui
AU  - Wang H
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan, 250014, 
      China.
FAU - Chen, Xinping
AU  - Chen X
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan, 250014, 
      China.
FAU - Yang, Guiwen
AU  - Yang G
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan, 250014, 
      China. yanggw@sdnu.edu.cn.
FAU - Li, Hua
AU  - Li H
AUID- ORCID: 0000-0001-6367-4764
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan, 250014, 
      China. lihua@sdnu.edu.cn.
LA  - eng
GR  - 2018YFD0900302-8/National Key R&D Program of China/
GR  - 31972828/National Natural Science Foundation of China/
GR  - ZR2018BCE054/Natural Science Foundation of Shandong Province/
PT  - Journal Article
DEP - 20201119
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (DNA, Complementary)
RN  - 0 (Fish Proteins)
RN  - 0 (Interferon Regulatory Factors)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Peptidoglycan)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Aeromonas hydrophila/immunology
MH  - Animals
MH  - Carps/*genetics/*immunology/metabolism
MH  - DNA, Complementary
MH  - Fish Proteins
MH  - Interferon Regulatory Factors/*genetics
MH  - Lipopolysaccharides/immunology
MH  - Peptidoglycan/immunology
MH  - Phylogeny
MH  - Poly I-C/immunology
MH  - Sequence Analysis, DNA
MH  - Tissue Distribution
PMC - PMC7678311
OTO - NOTNLM
OT  - Aeromonas hydrophila
OT  - Common carp (Cyprinus carpio L.)
OT  - IFN response
OT  - Interferon regulatory factor 10 (IRF10)
OT  - poly I:C
COIS- The authors declare that they have no competing interests.
EDAT- 2020/11/21 06:00
MHDA- 2021/06/04 06:00
PMCR- 2020/11/19
CRDT- 2020/11/20 05:29
PHST- 2020/08/25 00:00 [received]
PHST- 2020/11/09 00:00 [accepted]
PHST- 2020/11/20 05:29 [entrez]
PHST- 2020/11/21 06:00 [pubmed]
PHST- 2021/06/04 06:00 [medline]
PHST- 2020/11/19 00:00 [pmc-release]
AID - 10.1186/s12917-020-02674-z [pii]
AID - 2674 [pii]
AID - 10.1186/s12917-020-02674-z [doi]
PST - epublish
SO  - BMC Vet Res. 2020 Nov 19;16(1):450. doi: 10.1186/s12917-020-02674-z.