PMID- 33215446
OWN - NLM
STAT- MEDLINE
DCOM- 20210628
LR  - 20210628
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 24
IP  - 21
DP  - 2020 Nov
TI  - MiR-140 protects against myocardial ischemia-reperfusion injury by regulating 
      NF-kappaB pathway.
PG  - 11266-11272
LID - 23616 [pii]
LID - 10.26355/eurrev_202011_23616 [doi]
AB  - OBJECTIVE: The aim of this study was to investigate the effect of micro 
      ribonucleic acid (miR)-140 on rats with myocardial ischemia-reperfusion injury 
      (MIRI) through regulating the nuclear factor-kappaB (NF-kappaB) pathway. MATERIALS AND 
      METHODS: A total of 36 Sprague-Dawley rats were randomly divided into three 
      groups, including sham group (n=12), model group (n=12) and miR-140 mimics group 
      (n=12). In sham group, only thoracotomy was performed without 
      ischemia-reperfusion. In model group, the MIRI model was first established, 
      followed by intervention using normal saline. In miR-140 mimics group, the MIRI 
      model was first established as well, followed by intervention using miR-140 
      mimics. The content of serum creatine kinase (CK) and lactate dehydrogenase (LDH) 
      was detected, and the morphology of myocardial tissues was observed via 
      hematoxylin-eosin (HE) staining. Meanwhile, the relative protein expression of 
      NF-kappaB was determined using Western blotting. Quantitative polymerase chain 
      reaction (qPCR) was conducted to evaluate the expression of miR-140. The content 
      of interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) was determined via 
      enzyme-linked immunosorbent assay (ELISA). Furthermore, cell apoptosis was 
      detected via terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) assay. RESULTS: The content of serum CK and LDH rose 
      significantly in model group and miR-140 mimics group when compared with sham 
      group (p<0.05). However, it declined significantly in miR-140 mimics group 
      compared with model group (p<0.05). HE staining results showed that there were no 
      obvious abnormalities in the morphology of myocardial tissues in sham group. 
      However, there were injury and inflammatory infiltration in myocardial tissues in 
      model group. Meanwhile, the structure and morphology of myocardial tissues were 
      improved in miR-140 mimics group compared with those in model group. Western 
      blotting revealed that the relative protein expression of NF-kappaB was evidently 
      higher in model group and miR-140 mimics group than sham group (p<0.05). However, 
      it was remarkably lower in miR-140 mimics group than that in model group 
      (p<0.05). QPCR results demonstrated that the relative expression of miR-140 in 
      model group and miR-140 mimics group was obviously lower than sham group 
      (p<0.05). However, a markedly higher expression of miR-140 was observed in 
      miR-140 mimics group than model group (p<0.05). ELISA results indicated that 
      model group and miR-140 mimics group had remarkably higher content of IL-1beta and 
      TNF-alpha than sham group (p<0.05). However, miR-140 mimics group had remarkably 
      lower content of IL-1beta and TNF-alpha than model group (p<0.05). TUNEL assay indicated 
      that the apoptosis rate increased obviously in model group and miR-140 mimics 
      group compared with sham group (p<0.05). However, it declined significantly in 
      miR-140 mimics group compared with model group (p<0.05). CONCLUSIONS: MiR-140 
      suppresses inflammation and apoptosis in myocardial tissues of MIRI rats through 
      inhibiting the NF-kappaB signaling pathway, thereby exerting a cardioprotective 
      effect.
FAU - Hao, L-Y
AU  - Hao LY
AD  - Department of Cardiology, Shanxi Yuncheng Central Hospital, Yuncheng, China. 
      luyansxmu@163.com.
FAU - Lu, Y
AU  - Lu Y
FAU - Ma, Y-C
AU  - Ma YC
FAU - Wei, R-P
AU  - Wei RP
FAU - Jia, Y-P
AU  - Jia YP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (MIRN140 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Disease Models, Animal
MH  - Inflammation/metabolism/pathology
MH  - MicroRNAs/*metabolism
MH  - Myocardial Reperfusion Injury/*metabolism/pathology
MH  - NF-kappa B/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
EDAT- 2020/11/21 06:00
MHDA- 2021/06/29 06:00
CRDT- 2020/11/20 05:52
PHST- 2020/11/20 05:52 [entrez]
PHST- 2020/11/21 06:00 [pubmed]
PHST- 2021/06/29 06:00 [medline]
AID - 23616 [pii]
AID - 10.26355/eurrev_202011_23616 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2020 Nov;24(21):11266-11272. doi: 
      10.26355/eurrev_202011_23616.