PMID- 33217999
OWN - NLM
STAT- MEDLINE
DCOM- 20210304
LR  - 20210304
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 22
DP  - 2020 Nov 18
TI  - Effects of Tenascin C on the Integrity of Extracellular Matrix and Skin Aging.
LID - 10.3390/ijms21228693 [doi]
LID - 8693
AB  - Tenascin C (TNC) is an element of the extracellular matrix (ECM) of various 
      tissues, including the skin, and is involved in modulating ECM integrity and cell 
      physiology. Although skin aging is apparently associated with changes in the ECM, 
      little is known about the role of TNC in skin aging. In this study, we found that 
      the Tnc mRNA level was significantly reduced in the skin tissues of aged mice 
      compared with young mice, consistent with reduced TNC protein expression in aged 
      human skin. TNC-large (TNC-L; 330-kDa) and -small (TNC-S; 240-kDa) polypeptides 
      were observed in conditional media from primary dermal fibroblasts. Both 
      recombinant TNC polypeptides, corresponding to TNC-L and TNC-S, increased the 
      expression of type I collagen and reduced the expression of matrix 
      metalloproteinase-1 in fibroblasts. Treatment of fibroblasts with a recombinant 
      TNC polypeptide, corresponding to TNC-L, induced phosphorylation of SMAD2 and 
      SMAD3. TNC increased the level of transforming growth factor-beta1 (TGF-beta1) mRNA and 
      upregulated the expression of type I collagen by activating the TGF-beta signaling 
      pathway. In addition, TNC also promoted the expression of type I collagen in 
      fibroblasts embedded in a three-dimensional collagen matrix. Our findings suggest 
      that TNC contributes to the integrity of ECM in young skin and to prevention of 
      skin aging.
FAU - Choi, Young Eun
AU  - Choi YE
AUID- ORCID: 0000-0003-3815-4833
AD  - Department of Biochemistry, College of Life Science and Biotechnology, Yonsei 
      University, Seoul 03722, Korea.
FAU - Song, Min Ji
AU  - Song MJ
AUID- ORCID: 0000-0002-5252-4522
AD  - Department of Dermatology, Seoul National University College of Medicine, Seoul 
      03080, Korea.
FAU - Hara, Mari
AU  - Hara M
AD  - Department of Pathology and Matrix Biology, Mie University Graduate School of 
      Medicine, Tsu 514-8507, Japan.
FAU - Imanaka-Yoshida, Kyoko
AU  - Imanaka-Yoshida K
AUID- ORCID: 0000-0002-8919-7342
AD  - Department of Pathology and Matrix Biology, Mie University Graduate School of 
      Medicine, Tsu 514-8507, Japan.
AD  - Mie University Research Center for Matrix Biology, Tsu 514-8507, Japan.
FAU - Lee, Dong Hun
AU  - Lee DH
AD  - Department of Dermatology, Seoul National University College of Medicine, Seoul 
      03080, Korea.
AD  - Institute of Human-Environment Interface Biology, Medical Research Center, Seoul 
      National University, Seoul 03080, Korea.
FAU - Chung, Jin Ho
AU  - Chung JH
AD  - Department of Dermatology, Seoul National University College of Medicine, Seoul 
      03080, Korea.
AD  - Institute of Human-Environment Interface Biology, Medical Research Center, Seoul 
      National University, Seoul 03080, Korea.
FAU - Lee, Seung-Taek
AU  - Lee ST
AUID- ORCID: 0000-0001-7300-9784
AD  - Department of Biochemistry, College of Life Science and Biotechnology, Yonsei 
      University, Seoul 03722, Korea.
LA  - eng
PT  - Journal Article
DEP - 20201118
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (RNA, Messenger)
RN  - 0 (SMAD2 protein, human)
RN  - 0 (SMAD3 protein, human)
RN  - 0 (Smad2 Protein)
RN  - 0 (Smad2 protein, mouse)
RN  - 0 (Smad3 Protein)
RN  - 0 (Smad3 protein, mouse)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (TNC protein, human)
RN  - 0 (Tenascin)
RN  - 0 (Tgfb1 protein, mouse)
RN  - 0 (Tnc protein, mouse)
RN  - 0 (Transforming Growth Factor beta1)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 13)
RN  - EC 3.4.24.- (Mmp13 protein, mouse)
RN  - EC 3.4.24.7 (MMP1 protein, human)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Animals
MH  - Extracellular Matrix/genetics/*metabolism
MH  - Female
MH  - Humans
MH  - Matrix Metalloproteinase 1/genetics/metabolism
MH  - Matrix Metalloproteinase 13/genetics/metabolism
MH  - Mice
MH  - Mice, Hairless
MH  - RNA, Messenger/genetics/metabolism
MH  - *Signal Transduction
MH  - *Skin Aging
MH  - Smad2 Protein/genetics/metabolism
MH  - Smad3 Protein/genetics/metabolism
MH  - Tenascin/genetics/*metabolism
MH  - Transforming Growth Factor beta1/genetics/metabolism
PMC - PMC7698786
OTO - NOTNLM
OT  - TGF-beta
OT  - aging
OT  - collagen
OT  - extracellular matrix
OT  - fibroblast
OT  - skin
OT  - tenascin C
COIS- The authors declare no conflict of interest.
EDAT- 2020/11/22 06:00
MHDA- 2021/03/05 06:00
PMCR- 2020/11/01
CRDT- 2020/11/21 01:01
PHST- 2020/10/27 00:00 [received]
PHST- 2020/11/14 00:00 [revised]
PHST- 2020/11/16 00:00 [accepted]
PHST- 2020/11/21 01:01 [entrez]
PHST- 2020/11/22 06:00 [pubmed]
PHST- 2021/03/05 06:00 [medline]
PHST- 2020/11/01 00:00 [pmc-release]
AID - ijms21228693 [pii]
AID - ijms-21-08693 [pii]
AID - 10.3390/ijms21228693 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Nov 18;21(22):8693. doi: 10.3390/ijms21228693.