PMID- 33220541 OWN - NLM STAT- MEDLINE DCOM- 20201215 LR - 20221207 IS - 1873-2976 (Electronic) IS - 0960-8524 (Linking) VI - 320 IP - Pt B DP - 2021 Jan TI - Comprehensive performance, bacterial community structure of single-chamber microbial fuel cell affected by COD/N ratio and physiological stratifications in cathode biofilm. PG - 124416 LID - S0960-8524(20)31690-4 [pii] LID - 10.1016/j.biortech.2020.124416 [doi] AB - This study compares the effects and bacterial community structure of single-chamber microbial fuel cells (MFCs) in the treatment of NH(4)(+)-containing wastewater with different chemical oxygen demand (COD)/N ratios, whilst simultaneously conducting stratification research on the cathode biofilm. To this end, five nitrifier pre-enriched single-chamber MFC reactors are established to treat five different COD/N wastewaters, respectively. The results show that MFCs with low COD/N have better NH(4)(+)-N removal, electrochemical performance, but the removal stability and COD removal effect are lower than MFCs with high COD/N. High-throughput sequencing reveals that the anode community structure is weakly affected by the COD/N and is dominated by Geobacter; however, the cathode community is complex and susceptible to the COD/N. Furthermore, the pH profile in the cathode biofilm is characterized by a pH microelectrode and fluorescence in situ hybridization (FISH) is used to confirm that the distribution trend of nitrifiers and denitrifiers in cathode biofilm. CI - Copyright (c) 2020 Elsevier Ltd. All rights reserved. FAU - Yuan, Jianqi AU - Yuan J AD - School of Environment and Energy, South China University of Technology, Higher Education Mega Center, Guangzhou 510006, PR China; Guangdong Ecological Environment Control Engineering Technology Research Center, South China University of Technology, Higher Education Mega Center, Guangzhou 510006, PR China. FAU - Yuan, Haiguang AU - Yuan H AD - School of Environment and Energy, South China University of Technology, Higher Education Mega Center, Guangzhou 510006, PR China; Guangdong Ecological Environment Control Engineering Technology Research Center, South China University of Technology, Higher Education Mega Center, Guangzhou 510006, PR China. FAU - Huang, Shaobin AU - Huang S AD - School of Environment and Energy, South China University of Technology, Higher Education Mega Center, Guangzhou 510006, PR China; Guangdong Ecological Environment Control Engineering Technology Research Center, South China University of Technology, Higher Education Mega Center, Guangzhou 510006, PR China. Electronic address: chshuang@scut.edu.cn. FAU - Liu, Lijie AU - Liu L AD - School of Environment and Energy, South China University of Technology, Higher Education Mega Center, Guangzhou 510006, PR China; Guangdong Ecological Environment Control Engineering Technology Research Center, South China University of Technology, Higher Education Mega Center, Guangzhou 510006, PR China. FAU - Fu, Feichao AU - Fu F AD - School of Environment and Energy, South China University of Technology, Higher Education Mega Center, Guangzhou 510006, PR China; Guangdong Ecological Environment Control Engineering Technology Research Center, South China University of Technology, Higher Education Mega Center, Guangzhou 510006, PR China. FAU - Zhang, Yongqing AU - Zhang Y AD - School of Environment and Energy, South China University of Technology, Higher Education Mega Center, Guangzhou 510006, PR China. FAU - Cheng, Fangqin AU - Cheng F AD - Institute of Resources and Environmental Engineering, Shanxi Collaborative Innovation Center of High Value-added Utilization of Coal-related Wastes, Shanxi University, Taiyuan 030006, PR China. FAU - Li, Jianfeng AU - Li J AD - Institute of Resources and Environmental Engineering, Shanxi Collaborative Innovation Center of High Value-added Utilization of Coal-related Wastes, Shanxi University, Taiyuan 030006, PR China. LA - eng PT - Journal Article DEP - 20201116 PL - England TA - Bioresour Technol JT - Bioresource technology JID - 9889523 RN - 0 (Waste Water) SB - IM MH - *Bioelectric Energy Sources MH - Biofilms MH - Biological Oxygen Demand Analysis MH - Electricity MH - Electrodes MH - In Situ Hybridization, Fluorescence MH - Wastewater OTO - NOTNLM OT - Cathode biofilm OT - Denitrification OT - Microbial fuel cell OT - Microelectrode OT - Nitrification OT - Single-chamber EDAT- 2020/11/22 06:00 MHDA- 2020/12/16 06:00 CRDT- 2020/11/21 20:09 PHST- 2020/09/29 00:00 [received] PHST- 2020/11/09 00:00 [revised] PHST- 2020/11/11 00:00 [accepted] PHST- 2020/11/22 06:00 [pubmed] PHST- 2020/12/16 06:00 [medline] PHST- 2020/11/21 20:09 [entrez] AID - S0960-8524(20)31690-4 [pii] AID - 10.1016/j.biortech.2020.124416 [doi] PST - ppublish SO - Bioresour Technol. 2021 Jan;320(Pt B):124416. doi: 10.1016/j.biortech.2020.124416. Epub 2020 Nov 16.