PMID- 3322100
OWN - NLM
STAT- MEDLINE
DCOM- 19880122
LR  - 20190628
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 165
IP  - 2
DP  - 1987 Sep
TI  - Radioenzymatic determination of phenylpropanolamine in plasma.
PG  - 275-86
AB  - We modified a norepinephrine radioenzymatic method (C. R. Lake, M. G. Ziegler, 
      and I. J. Kopin, 1976, Life Sci. 18, 1315-1326) for determination of plasma 
      phenylpropanolamine (PPA) concentrations. PPA is converted to 
      N[methyl-3H]ephedrine ([3H]EPD) by the enzyme phenylethanolamine 
      N-methyltransferase (PNMT) and S-[methyl-3H]adenosyl-L-methionine ([3H]AdoMet). 
      The product, [3H]EPD, is isolated from unreacted [3H]AdoMet and labeled side 
      products by an organic extraction and a TLC procedure. In addition, a 
      preincubation organic extraction procedure is included to remove inhibitors of 
      PNMT from plasma and to concentrate the sample for enhanced enzymatic conversion. 
      In order to accurately quantitate PPA across the wide range of possible 
      concentrations, the assay is conducted at two plasma volumes. PPA concentrations 
      between 0.3 and 50 micrograms/liter can be detected with 1 ml of plasma, while 
      concentrations between 4 and 1500 micrograms/liter can be detected with 0.1 ml of 
      plasma. The intra-assay coefficients of variation (CVs) are 9.3 and 5.7% at 0.5 
      and 1500 micrograms/liter, respectively, while the mean interassay CV is 13.8%.
FAU - Reid, A A
AU  - Reid AA
AD  - Department of Psychiatry, Uniformed Services University of the Health Sciences, 
      F. Edward Hebert School of Medicine, Bethesda, Maryland 20814.
FAU - Fleming, P J
AU  - Fleming PJ
FAU - Lake, C R
AU  - Lake CR
LA  - eng
GR  - R01 DA 03513/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 10028-17-8 (Tritium)
RN  - 33RU150WUN (Phenylpropanolamine)
RN  - EC 2.1.1.28 (Phenylethanolamine N-Methyltransferase)
SB  - IM
MH  - Humans
MH  - Isomerism
MH  - Kinetics
MH  - Phenylethanolamine N-Methyltransferase
MH  - Phenylpropanolamine/*blood
MH  - Radioisotope Dilution Technique
MH  - Tritium
EDAT- 1987/09/01 00:00
MHDA- 1987/09/01 00:01
CRDT- 1987/09/01 00:00
PHST- 1987/09/01 00:00 [pubmed]
PHST- 1987/09/01 00:01 [medline]
PHST- 1987/09/01 00:00 [entrez]
AID - 0003-2697(87)90270-3 [pii]
AID - 10.1016/0003-2697(87)90270-3 [doi]
PST - ppublish
SO  - Anal Biochem. 1987 Sep;165(2):275-86. doi: 10.1016/0003-2697(87)90270-3.