PMID- 33226590
OWN - NLM
STAT- MEDLINE
DCOM- 20210326
LR  - 20210326
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2223
DP  - 2021
TI  - Induction of Airway Hypersensitivity to Ovalbumin and Dust Mite Allergens as 
      Mouse Models of Allergic Asthma.
PG  - 101-114
LID - 10.1007/978-1-0716-1001-5_8 [doi]
AB  - Mouse models of allergic asthma have been utilized to establish the role of T 
      helper type 2 (Th2) cells in driving lung inflammation, airway 
      hyperresponsiveness, and obstruction. Here, we present the allergic asthma 
      models, in which mice are hypersensitized to ovalbumin (OVA) and house dust mite 
      (HDM). These models mimic the major characteristics of human asthma including the 
      eosinophilic inflammation and hyperactivity of the airway, overproduction of Th2 
      cytokines in the lung, and elevated total and allergen-specific immunoglobulin E 
      (IgE) in serum.
FAU - Chen, Mei-Chi
AU  - Chen MC
AD  - Division of Biological Sciences, University of California, San Diego, La Jolla, 
      CA, USA.
FAU - Tai, Jesse W
AU  - Tai JW
AD  - Division of Biological Sciences, University of California, San Diego, La Jolla, 
      CA, USA.
FAU - Wu, Cheng-Jang
AU  - Wu CJ
AD  - Division of Biological Sciences, University of California, San Diego, La Jolla, 
      CA, USA. chw161@ucsd.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Allergens)
RN  - 0 (Biomarkers)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Foxp3 protein, mouse)
RN  - 0 (Interleukins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 5QB0T2IUN0 (Aluminum Hydroxide)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Adjuvants, Immunologic/administration & dosage
MH  - Allergens/administration & dosage
MH  - Aluminum Hydroxide/administration & dosage
MH  - Animals
MH  - Asthma/chemically induced/genetics/*immunology/pathology
MH  - Biomarkers/metabolism
MH  - Dendritic Cells/*drug effects/immunology/pathology
MH  - *Disease Models, Animal
MH  - Eosinophils/drug effects/immunology/pathology
MH  - Flow Cytometry/methods
MH  - Forkhead Transcription Factors/genetics/immunology
MH  - Gene Expression
MH  - Immunoglobulin E/genetics/immunology
MH  - Interferon-gamma/genetics/immunology
MH  - Interleukins/genetics/immunology
MH  - Lung/drug effects/immunology/pathology
MH  - Macrophages/drug effects/immunology/pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Ovalbumin/*administration & dosage
MH  - Pyroglyphidae/chemistry/*immunology
MH  - Respiratory Function Tests
MH  - Respiratory Hypersensitivity/chemically induced/genetics/*immunology/pathology
MH  - Th2 Cells/*drug effects/immunology/pathology
OTO - NOTNLM
OT  - Airway hypersensitivity
OT  - Allergic asthma
OT  - House dust mite
OT  - Immunoglobulin E
OT  - Mouse model
OT  - Ovalbumin
OT  - Pulmonary eosinophilia
OT  - T helper type 2 cytokines
EDAT- 2020/11/24 06:00
MHDA- 2021/03/27 06:00
CRDT- 2020/11/23 12:12
PHST- 2020/11/23 12:12 [entrez]
PHST- 2020/11/24 06:00 [pubmed]
PHST- 2021/03/27 06:00 [medline]
AID - 10.1007/978-1-0716-1001-5_8 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2021;2223:101-114. doi: 10.1007/978-1-0716-1001-5_8.