PMID- 33226813
OWN - NLM
STAT- MEDLINE
DCOM- 20210617
LR  - 20210617
IS  - 1535-3907 (Electronic)
IS  - 1535-3893 (Linking)
VI  - 20
IP  - 1
DP  - 2021 Jan 1
TI  - Proteomic Analysis Reveals the Effect of Trichostatin A and Bone Marrow-Derived 
      Dendritic Cells on the Fatty Acid Metabolism of NIH3T3 Cells under Oxygen-Glucose 
      Deprivation Conditions.
PG  - 960-971
LID - 10.1021/acs.jproteome.0c00713 [doi]
AB  - Fibroblasts mediate acute wound healing and long-term tissue remodeling with 
      scarring after tissue injury. Following myocardial infarction (MI), necrotized 
      cardiomyocytes become replaced by secreted extracellular matrix proteins produced 
      by fibroblasts. Dendritic cells (DCs) can migrate from the bone marrow to the 
      infarct areas and infarct border areas to mediate collagen accumulation after MI. 
      Trichostatin A (TSA) is known to regulate apoptosis and proliferation in 
      fibroblasts and affect the functions of DCs under oxygen-glucose deprivation 
      (OGD) conditions. In this study, we used label-free quantitative proteomics to 
      investigate the effects of TSA and bone marrow-derived dendritic cells (BMDCs) on 
      NIH3T3 fibroblasts under OGD conditions. The results showed that the fatty acid 
      degradation pathway was significantly upregulated in NIH3T3 cells under OGD 
      conditions and that the fatty acid synthesis pathway was significantly 
      downregulated in NIH3T3 cells treated with conditioned media (CM) from BMDCs 
      treated with TSA under OGD conditions [BMDCs-CM(TSA)]. In addition, BMDCs-CM(TSA) 
      significantly decreased the levels of triglycerides and free fatty acids and 
      mediated fatty acid metabolism-related proteins in NIH3T3 cells under OGD 
      conditions. In summary, this proteomics analysis showed that TSA and BMDCs affect 
      fatty acid metabolism in NIH3T3 cells under OGD conditions.
FAU - Wang, Xuan
AU  - Wang X
AD  - Department of Pharmacology, College of Basic Medical Sciences, Jilin University, 
      Changchun 130000, China.
FAU - Song, Tongtong
AU  - Song T
AD  - Department of Pharmacology, College of Basic Medical Sciences, Jilin University, 
      Changchun 130000, China.
FAU - Sun, Yunpeng
AU  - Sun Y
AD  - Cardiac Surgery Department, The First Hospital of Jilin University, Changchun 
      130000, China.
FAU - Men, Lihui
AU  - Men L
AD  - Department of Pharmacology, College of Basic Medical Sciences, Jilin University, 
      Changchun 130000, China.
FAU - Gu, Yiwen
AU  - Gu Y
AD  - Department of Pharmacology, College of Basic Medical Sciences, Jilin University, 
      Changchun 130000, China.
FAU - Zhang, Siwei
AU  - Zhang S
AD  - Department of Pharmacology, College of Basic Medical Sciences, Jilin University, 
      Changchun 130000, China.
FAU - Chen, Xia
AU  - Chen X
AUID- ORCID: 0000-0003-3732-073X
AD  - Department of Pharmacology, College of Basic Medical Sciences, Jilin University, 
      Changchun 130000, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201123
PL  - United States
TA  - J Proteome Res
JT  - Journal of proteome research
JID - 101128775
RN  - 0 (Fatty Acids)
RN  - 0 (Hydroxamic Acids)
RN  - 3X2S926L3Z (trichostatin A)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Bone Marrow
MH  - Dendritic Cells
MH  - Fatty Acids
MH  - *Glucose
MH  - Hydroxamic Acids
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Oxygen
MH  - *Proteomics
OTO - NOTNLM
OT  - BMDCs
OT  - NIH3T3 cells
OT  - TSA
OT  - fatty acid metabolism
EDAT- 2020/11/24 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/11/23 17:08
PHST- 2020/11/24 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/11/23 17:08 [entrez]
AID - 10.1021/acs.jproteome.0c00713 [doi]
PST - ppublish
SO  - J Proteome Res. 2021 Jan 1;20(1):960-971. doi: 10.1021/acs.jproteome.0c00713. 
      Epub 2020 Nov 23.