PMID- 33227189
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201210
IS  - 2233-8233 (Print)
IS  - 2233-8241 (Electronic)
IS  - 2093-8896 (Linking)
VI  - 47
IP  - 4
DP  - 2020 Dec
TI  - Reanalysis of discarded blastocysts for autosomal aneuploidy after sex selection 
      in cleavage-stage embryos.
PG  - 293-299
LID - 10.5653/cerm.2019.03426 [doi]
AB  - OBJECTIVE: The goal of the present study was to investigate the rate of 
      chromosomal aneuploidies in surplus embryos after sex determination at the 
      cleavage stage. Then, the same chromosomal aneuploidies were evaluated in 
      blastocysts after extended culture. METHODS: Sixty-eight surplus embryos were 
      biopsied at the cleavage stage and incubated for an additional 3 days to allow 
      them to reach the blastocyst stage. The embryos were reanalyzed via fluorescence 
      in situ hybridization (FISH) to examine eight chromosomes (13, 15, 16, 18, 21, 
      22, X, and Y) in both cleavage-stage embryos and blastocysts. RESULTS: Although 
      the total abnormality rate was lower in blastocysts (32.35%) than in 
      cleavage-stage embryos (45.58%), the difference was not significant (p=0.113). 
      However, when we restricted the analysis to autosomal abnormalities, we observed 
      a significant difference in the abnormality rate between the cleavage-stage 
      embryos (44.11%) and the blastocysts (17.64%, p=0.008). A higher rate of sex 
      chromosomal abnormalities was also observed in cleavage-stage embryos (29.4%) 
      than in blastocysts (14.70%, p=0.038). CONCLUSION: The data indicated that embryo 
      biopsy should be conducted at the blastocyst stage rather than the cleavage 
      stage. The results also emphasized that examination of common chromosomal 
      aneuploidies apart from sex selection cycles can be conducted in the blastocyst 
      stage with the FISH method.
FAU - Ebrahimian, Neda
AU  - Ebrahimian N
AD  - Research and Clinical Center for Infertility, Yazd Reproductive Sciences 
      Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
AD  - Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi 
      University of Medical Sciences, Yazd, Iran.
FAU - Montazeri, Fatemeh
AU  - Montazeri F
AD  - Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi 
      University of Medical Sciences, Yazd, Iran.
FAU - Sadeghi, Mohammad Reza
AU  - Sadeghi MR
AD  - Reproductive Embryology and Andrology Research Center, Avicenna Research 
      Institute, Academic Center for Education, Culture and Research, Tehran, Iran.
FAU - Kalantar, Seyed Mehdi
AU  - Kalantar SM
AD  - Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi 
      University of Medical Sciences, Yazd, Iran.
FAU - Gilany, Kambiz
AU  - Gilany K
AD  - Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, 
      Tehran, Iran.
FAU - Khalili, Mohannad Ali
AU  - Khalili MA
AD  - Research and Clinical Center for Infertility, Yazd Reproductive Sciences 
      Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
LA  - eng
GR  - grant no. 5647/Yazd Reproductive Sciences Institute/
PT  - Journal Article
DEP - 20201119
PL  - Korea (South)
TA  - Clin Exp Reprod Med
JT  - Clinical and experimental reproductive medicine
JID - 101563916
PMC - PMC7711103
OTO - NOTNLM
OT  - Blastocyst
OT  - Embryo
OT  - Fluorescence in situ hybridization
OT  - Trophectoderm biopsy
COIS- Conflict of interest No potential conflict of interest relevant to this article 
      was reported.
EDAT- 2020/11/24 06:00
MHDA- 2020/11/24 06:01
PMCR- 2020/12/01
CRDT- 2020/11/23 18:36
PHST- 2019/11/21 00:00 [received]
PHST- 2020/06/11 00:00 [accepted]
PHST- 2020/11/24 06:00 [pubmed]
PHST- 2020/11/24 06:01 [medline]
PHST- 2020/11/23 18:36 [entrez]
PHST- 2020/12/01 00:00 [pmc-release]
AID - cerm.2019.03426 [pii]
AID - cerm-2019-03426 [pii]
AID - 10.5653/cerm.2019.03426 [doi]
PST - ppublish
SO  - Clin Exp Reprod Med. 2020 Dec;47(4):293-299. doi: 10.5653/cerm.2019.03426. Epub 
      2020 Nov 19.