PMID- 33227421
OWN - NLM
STAT- MEDLINE
DCOM- 20210524
LR  - 20210524
IS  - 1879-1107 (Electronic)
IS  - 1096-4959 (Linking)
VI  - 252
DP  - 2021 Feb-Mar
TI  - Short communication: Tissue-specific transcript expression of P-glycoprotein 
      isoforms abcb1a and abcb1b in rainbow trout (Oncorhynchus mykiss) following 
      induction with clotrimazole.
PG  - 110538
LID - S1096-4959(20)30132-9 [pii]
LID - 10.1016/j.cbpb.2020.110538 [doi]
AB  - P-glycoprotein (P-gp) plays a pivotal role in cellular defense, aimed at reducing 
      xenobiotic accumulation. As a member of the ABC family of proteins, expression of 
      this protein confers the multixenobiotic resistant (MXR) phenotype in aquatic 
      organisms, including fish. To identify tissues protected by or contributing to 
      the elimination of xenobiotics via P-gp, tissue-specific P-gp isoforms abcb1a and 
      abcb1b transcript expression were measured in rainbow trout (Oncorhynchus 
      mykiss). Tissues investigated included the proximal and distal intestines, liver, 
      head kidney, gills, gonads, and 5 regions of the brain: olfactory lobe, cerebrum, 
      optic lobe, cerebellum and medulla. Abcb1a transcript was more widely expressed 
      across tissues and generally showed higher transcript expression than abcb1b. 
      Deviation from this trend occurred in the gills, cerebrum and head kidney, where 
      transcript levels were relatively equal between abcb1a and abcb1b. Intestinal 
      tissues had greater abcb1a expression than abcb1b (3 orders of magnitude). Abcb1b 
      was absent from liver tissue indicating that abcb1a is relied upon for hepatic 
      defense. This study suggests that abcb1b acts to protect sensitive organs from 
      compounds in the systemic circulation (brain and gonad), whereas abcb1a acts 
      primarily in an elimination role in organs such as liver and intestine. To 
      determine if P-gp induction alters transcript responses, the antifungal mammalian 
      Pregnane-X-Receptor (PXR) agonist clotrimazole (CTZ) was used. CTZ-treated 
      rainbow trout showed significantly increased abcb1b transcript expression in the 
      optic lobe and distal intestine, providing evidence that trout PXR exhibits a 
      similar substrate base as mammalian PXR, albeit selectively in regions of the 
      brain and intestine.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Love, Ryan C
AU  - Love RC
AD  - Department of Biological Sciences, Simon Fraser University, 8888 University 
      Drive, Burnaby, British Columbia V5A 1S6, Canada.
FAU - Osachoff, Heather L
AU  - Osachoff HL
AD  - Department of Biological Sciences, Simon Fraser University, 8888 University 
      Drive, Burnaby, British Columbia V5A 1S6, Canada.
FAU - Kennedy, Christopher J
AU  - Kennedy CJ
AD  - Department of Biological Sciences, Simon Fraser University, 8888 University 
      Drive, Burnaby, British Columbia V5A 1S6, Canada. Electronic address: 
      ckennedy@sfu.ca.
LA  - eng
PT  - Journal Article
DEP - 20201121
PL  - England
TA  - Comp Biochem Physiol B Biochem Mol Biol
JT  - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
JID - 9516061
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 1)
RN  - 0 (Antifungal Agents)
RN  - 0 (Fish Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - G07GZ97H65 (Clotrimazole)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 1/genetics/*metabolism
MH  - Animals
MH  - Antifungal Agents/pharmacology
MH  - Clotrimazole/*pharmacology
MH  - Fish Proteins/genetics/*metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Gills/drug effects/metabolism
MH  - Liver/drug effects/metabolism
MH  - Oncorhynchus mykiss/genetics/*metabolism
MH  - Organ Specificity
MH  - Protein Isoforms
MH  - RNA, Messenger/genetics/*metabolism
OTO - NOTNLM
OT  - ABC transporter
OT  - Clotrimazole
OT  - Induction
OT  - P-glycoprotein
OT  - Rainbow trout
EDAT- 2020/11/24 06:00
MHDA- 2021/05/25 06:00
CRDT- 2020/11/23 20:09
PHST- 2020/09/16 00:00 [received]
PHST- 2020/11/12 00:00 [revised]
PHST- 2020/11/13 00:00 [accepted]
PHST- 2020/11/24 06:00 [pubmed]
PHST- 2021/05/25 06:00 [medline]
PHST- 2020/11/23 20:09 [entrez]
AID - S1096-4959(20)30132-9 [pii]
AID - 10.1016/j.cbpb.2020.110538 [doi]
PST - ppublish
SO  - Comp Biochem Physiol B Biochem Mol Biol. 2021 Feb-Mar;252:110538. doi: 
      10.1016/j.cbpb.2020.110538. Epub 2020 Nov 21.