PMID- 33229071
OWN - NLM
STAT- MEDLINE
DCOM- 20210216
LR  - 20210216
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 129
DP  - 2021 Jan
TI  - HtrA2 is required for inflammatory responses in BMDMs via controlling TRAF2 
      stability in collagen-induced arthritis.
PG  - 78-85
LID - S0161-5890(20)30529-0 [pii]
LID - 10.1016/j.molimm.2020.10.024 [doi]
AB  - Rheumatoid arthritis (RA) is an autoimmune inflammatory disease characterized by 
      the destruction of cartilage and bone. The present study aims to investigate the 
      role of HtrA serine peptidase 2 (HtrA2) in the collagen-induced arthritis. The 
      expressions of HtrA2 were determined in the database BioGPS and bone 
      marrow-derived macrophages (BMDMs). The populations of myeloid and lymphoid cells 
      were determined in wild type and HtrA2 knockout (HtrA2(MKO)) mice using flow 
      cytometry. In addition, the expressions of pro-inflammatory cytokines (Il6, Tnf, 
      and Il1beta) were determined in the activated BMDMs from wild type (WT) and 
      HtrA2(MKO) mice. STRING database was used to predict the interactive proteins of 
      HtrA2 and Co-Immunoprecipitation was used to confirm these interactions. A 
      collagen-induced arthritis model was established to investigate the effects of 
      HtrA2 on the arthritis symptoms. It was found that HtrA2 reduction was associated 
      with the activation of myeloid cells. Interestingly, HtrA2 deficiency did not 
      affect the development of myeloid and lymphoid cells. Further studies 
      demonstrated that HtrA2 deficiency suppressed the production of pro-inflammatory 
      cytokines in BMDMs induced by lipopolysaccharide or CpG. Co-Immunoprecipitation 
      results demonstrated that HtrA2 enhanced the stability of TNF receptor-associated 
      factor 2 (TRAF2). HtrA2 participated in the activation of the inflammatory 
      response in a collagen-induced arthritis model. In summary, HtrA2 modulates 
      inflammatory responses in BMDMs by controlling TRAF2 stability in a 
      collagen-induced arthritis mouse model.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Xu, Zhitong
AU  - Xu Z
AD  - Department of Orthopedics, Quanzhou First Hospital Affiliated to Fujian Medical 
      University, Quanzhou, 362000, Fujian, China.
FAU - Lin, Jinding
AU  - Lin J
AD  - Department of Orthopedics, Quanzhou First Hospital Affiliated to Fujian Medical 
      University, Quanzhou, 362000, Fujian, China.
FAU - Xie, Yongsong
AU  - Xie Y
AD  - Department of Orthopedics, Quanzhou First Hospital Affiliated to Fujian Medical 
      University, Quanzhou, 362000, Fujian, China.
FAU - Tang, Haifeng
AU  - Tang H
AD  - Department of Orthopedics, Quanzhou First Hospital Affiliated to Fujian Medical 
      University, Quanzhou, 362000, Fujian, China.
FAU - Xie, Junjie
AU  - Xie J
AD  - Department of Orthopedics, Quanzhou First Hospital Affiliated to Fujian Medical 
      University, Quanzhou, 362000, Fujian, China.
FAU - Zeng, Rongdong
AU  - Zeng R
AD  - Department of Orthopedics, Quanzhou First Hospital Affiliated to Fujian Medical 
      University, Quanzhou, 362000, Fujian, China. Electronic address: 
      zengrongdg@126.com.
LA  - eng
PT  - Journal Article
DEP - 20201120
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (Cytokines)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - 0 (TRAF2 protein, mouse)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.21.108 (High-Temperature Requirement A Serine Peptidase 2)
RN  - EC 3.4.21.108 (Htra2 protein, mouse)
SB  - IM
MH  - Animals
MH  - Arthritis, Experimental/chemically induced/metabolism
MH  - Arthritis, Rheumatoid/*chemically induced/*metabolism
MH  - Autoimmune Diseases/chemically induced/metabolism
MH  - Bone and Bones/metabolism
MH  - Cartilage/metabolism
MH  - Cells, Cultured
MH  - Collagen/*pharmacology
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - High-Temperature Requirement A Serine Peptidase 2/*metabolism
MH  - Inflammation/*metabolism
MH  - Lymphocytes/metabolism
MH  - Macrophages/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Myeloid Cells/metabolism
MH  - TNF Receptor-Associated Factor 2/*metabolism
OTO - NOTNLM
OT  - Arthritis
OT  - BMDMs
OT  - HtrA2
OT  - Inflammation
OT  - TRAF2
EDAT- 2020/11/25 06:00
MHDA- 2021/02/17 06:00
CRDT- 2020/11/24 05:45
PHST- 2020/08/28 00:00 [received]
PHST- 2020/09/29 00:00 [revised]
PHST- 2020/10/27 00:00 [accepted]
PHST- 2020/11/25 06:00 [pubmed]
PHST- 2021/02/17 06:00 [medline]
PHST- 2020/11/24 05:45 [entrez]
AID - S0161-5890(20)30529-0 [pii]
AID - 10.1016/j.molimm.2020.10.024 [doi]
PST - ppublish
SO  - Mol Immunol. 2021 Jan;129:78-85. doi: 10.1016/j.molimm.2020.10.024. Epub 2020 Nov 
      20.