PMID- 33230615
OWN - NLM
STAT- MEDLINE
DCOM- 20210701
LR  - 20220102
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Print)
IS  - 1058-0468 (Linking)
VI  - 38
IP  - 1
DP  - 2021 Jan
TI  - Conventional ICSI improves the euploid embryo rate in male reciprocal 
      translocation carriers.
PG  - 129-138
LID - 10.1007/s10815-020-02013-z [doi]
AB  - PURPOSE: To evaluate whether the morphologically normal spermatozoa selected for 
      intracytoplasmic sperm injection (ICSI) under microscope had a higher rate of 
      normal/balanced chromosome contents than that in the whole unselected sperm from 
      reciprocal translocation carriers. METHODS: Five hundred unselected spermatozoa 
      from each of 40 male translocation carriers were performed with fluorescence in 
      situ hybridization (FISH), to determine the rates of gametes with different 
      meiotic contents of translocated chromosomes. Meanwhile, 3030 biopsied 
      blastocysts from 239 male and 293 female reciprocal translocation carriers were 
      detected with the microarray technique to analyze the rates of embryos with 
      different translocated chromosome contents. RESULTS: The D3 embryo rate, 
      blastocyst formation rate, and euploid rate of blastocysts were remarkably higher 
      in male carriers than those in female (p = 0.001, p = 0.004, and p = 0.035, 
      respectively). In addition, the percentages of alternate products, which 
      contained normal/balanced chromosome contents, in embryos from male carriers were 
      markedly higher than those in sperm FISH (p = 2.48 x 10(-5) and 
      p = 2.88 x 10(-10)), while the percentages of adjacent-2 and 3:1 products were 
      lower than those in sperm FISH (p = 0.003 and p = 5.28 x 10(-44)). Moreover, 
      consistent results were obtained when comparing the rates of products in embryos 
      between male and female carriers. Specifically, the incidence of alternate 
      products in male carriers was higher than those in female carriers (p = 0.022). 
      However, no similar differences were seen between sperm and embryos of female 
      carriers. CONCLUSION: ICSI facilitates the selection of spermatozoa with 
      normal/balanced chromosome contents and improves the D3 embryo rate, blastocyst 
      formation rate, and the euploid embryo rate in male carriers.
FAU - Lei, Caixia
AU  - Lei C
AD  - Shanghai Ji Ai Genetics & IVF Institute, Obstetrics and Gynecology Hospital, 
      Fudan University, Shanghai, 200011, China.
FAU - Zhang, Shuo
AU  - Zhang S
AD  - Shanghai Ji Ai Genetics & IVF Institute, Obstetrics and Gynecology Hospital, 
      Fudan University, Shanghai, 200011, China.
FAU - Zhu, Saijuan
AU  - Zhu S
AD  - Shanghai Ji Ai Genetics & IVF Institute, Obstetrics and Gynecology Hospital, 
      Fudan University, Shanghai, 200011, China.
FAU - Wu, Junping
AU  - Wu J
AD  - Shanghai Ji Ai Genetics & IVF Institute, Obstetrics and Gynecology Hospital, 
      Fudan University, Shanghai, 200011, China.
FAU - Xiao, Min
AU  - Xiao M
AD  - Shanghai Ji Ai Genetics & IVF Institute, Obstetrics and Gynecology Hospital, 
      Fudan University, Shanghai, 200011, China.
FAU - Zhou, Jing
AU  - Zhou J
AD  - Shanghai Ji Ai Genetics & IVF Institute, Obstetrics and Gynecology Hospital, 
      Fudan University, Shanghai, 200011, China.
FAU - Fu, Jing
AU  - Fu J
AD  - Shanghai Ji Ai Genetics & IVF Institute, Obstetrics and Gynecology Hospital, 
      Fudan University, Shanghai, 200011, China.
FAU - Sun, Yijuan
AU  - Sun Y
AD  - Shanghai Ji Ai Genetics & IVF Institute, Obstetrics and Gynecology Hospital, 
      Fudan University, Shanghai, 200011, China.
FAU - Xu, Congjian
AU  - Xu C
AD  - Shanghai Ji Ai Genetics & IVF Institute, Obstetrics and Gynecology Hospital, 
      Fudan University, Shanghai, 200011, China.
AD  - Key Laboratory of Female Reproductive Endocrine Related Diseases, Obstetrics and 
      Gynecology Hospital, Fudan University, Shanghai, 200011, China.
FAU - Sun, Xiaoxi
AU  - Sun X
AUID- ORCID: 0000-0002-3366-5821
AD  - Shanghai Ji Ai Genetics & IVF Institute, Obstetrics and Gynecology Hospital, 
      Fudan University, Shanghai, 200011, China. xiaoxi_sun@aliyun.com.
AD  - Key Laboratory of Female Reproductive Endocrine Related Diseases, Obstetrics and 
      Gynecology Hospital, Fudan University, Shanghai, 200011, China. 
      xiaoxi_sun@aliyun.com.
AD  - , Shanghai, China. xiaoxi_sun@aliyun.com.
LA  - eng
GR  - 18411953800/the Science and Technology Innovation Action Plan Program of 
      Shanghai/
GR  - SHDC12017105/Shanghai Shen Kang Hospital Development Centre Municipal Hospital 
      New Frontier Technology Joint Project/
PT  - Journal Article
DEP - 20201123
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
SB  - IM
CIN - J Assist Reprod Genet. 2021 May;38(5):1255-1256. PMID: 33686545
MH  - Adult
MH  - Blastocyst/metabolism
MH  - Chromosome Segregation/*genetics
MH  - Embryo Transfer/methods
MH  - Female
MH  - Heterozygote
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Meiosis/genetics
MH  - Pregnancy
MH  - Pregnancy Rate
MH  - *Preimplantation Diagnosis
MH  - Sperm Injections, Intracytoplasmic/*methods
MH  - Spermatozoa/growth & development
MH  - Translocation, Genetic/*genetics
PMC - PMC7822975
OTO - NOTNLM
OT  - Alternate products
OT  - ICSI
OT  - Male reciprocal translocation
OT  - Quadrivalent structure
OT  - Segregation patterns
OT  - Sperm FISH
COIS- The authors declare that they have no conflict of interest.
EDAT- 2020/11/25 06:00
MHDA- 2021/07/02 06:00
PMCR- 2022/01/01
CRDT- 2020/11/24 06:06
PHST- 2020/07/07 00:00 [received]
PHST- 2020/11/10 00:00 [accepted]
PHST- 2020/11/25 06:00 [pubmed]
PHST- 2021/07/02 06:00 [medline]
PHST- 2020/11/24 06:06 [entrez]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.1007/s10815-020-02013-z [pii]
AID - 2013 [pii]
AID - 10.1007/s10815-020-02013-z [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2021 Jan;38(1):129-138. doi: 10.1007/s10815-020-02013-z. 
      Epub 2020 Nov 23.