PMID- 33230951
OWN - NLM
STAT- MEDLINE
DCOM- 20210624
LR  - 20210624
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 35
IP  - 2
DP  - 2021 Feb
TI  - Intermittent short-duration reoxygenation protects against simulated high 
      altitude-induced pulmonary hypertension in rats.
PG  - e21212
LID - 10.1096/fj.202000533RR [doi]
AB  - High-altitude pulmonary hypertension (HAPH) is a severe and progressive disease 
      caused by chronic hypoxia and subsequent pulmonary vascular remodeling. No cure 
      is currently available owing to an incomplete understanding about vascular 
      remodeling. It is believed that hypoxia-induced diseases can be prevented by 
      treating hypoxia. Thus, this study aimed to determine whether daily 
      short-duration reoxygenation at sea level attenuates pulmonary hypertension under 
      high-altitude hypoxia. To this end, a simulated 5000-m hypoxia rat model and 
      hypoxic cultured human pulmonary artery smooth muscle cells were used to evaluate 
      the effect of short-duration reoxygenation. Results show that intermittent, not 
      continuous, short-duration reoxygenation effectively attenuates hypoxia-induced 
      pulmonary hypertension. The mechanisms underlining the protective effects 
      involved that intermittent, short-duration reoxygenation prevented functional and 
      structural remodeling of pulmonary arteries and proliferation, migration, and 
      phenotypic conversion of pulmonary artery smooth muscle cells under hypoxia. The 
      specific genes or potential molecular pathways responsible for mediating the 
      protective effects were also characterised by RNA sequencing. Further, the 
      frequency and the total time of intermittent reoxygenation affected its 
      preventive effect of HAPH, which was likely attributable to augmented oxidative 
      stress. Hence, daily intermittent, not continuous, short-duration reoxygenation 
      partially prevented pulmonary hypertension induced by 5000-m hypoxia in rats. 
      This study is novel in revealing a new potential method in preventing HAPH. It 
      gives insights into the selection and optimisation of oxygen supply schemes in 
      high-altitude areas.
CI  - (c) 2020 The Authors. The FASEB Journal published by Wiley Periodicals LLC on 
      behalf of Federation of American Societies for Experimental Biology.
FAU - Lyu, Qiang
AU  - Lyu Q
AD  - Department of Aerospace Physiology, Fourth Military Medical University, Xi'an, 
      China.
FAU - Bai, Yungang
AU  - Bai Y
AD  - Department of Aerospace Physiology, Fourth Military Medical University, Xi'an, 
      China.
FAU - Cheng, Jiuhua
AU  - Cheng J
AD  - Department of Aerospace Physiology, Fourth Military Medical University, Xi'an, 
      China.
FAU - Liu, Huan
AU  - Liu H
AD  - Department of Aerospace Physiology, Fourth Military Medical University, Xi'an, 
      China.
FAU - Li, Shaohua
AU  - Li S
AD  - Department of Aerospace Physiology, Fourth Military Medical University, Xi'an, 
      China.
FAU - Yang, Jing
AU  - Yang J
AD  - Department of Aerospace Physiology, Fourth Military Medical University, Xi'an, 
      China.
FAU - Wang, Zhongchao
AU  - Wang Z
AUID- ORCID: 0000-0003-3118-3375
AD  - Department of Aerospace Physiology, Fourth Military Medical University, Xi'an, 
      China.
FAU - Ma, Yan
AU  - Ma Y
AD  - Department of Aerospace Physiology, Fourth Military Medical University, Xi'an, 
      China.
FAU - Jiang, Min
AU  - Jiang M
AD  - Department of Aerospace Physiology, Fourth Military Medical University, Xi'an, 
      China.
FAU - Dong, Dong
AU  - Dong D
AD  - Department of Aerospace Physiology, Fourth Military Medical University, Xi'an, 
      China.
FAU - Yan, Yiquan
AU  - Yan Y
AD  - Department of Aerospace Physiology, Fourth Military Medical University, Xi'an, 
      China.
FAU - Shi, Qixin
AU  - Shi Q
AD  - Department of Aerospace Physiology, Fourth Military Medical University, Xi'an, 
      China.
FAU - Ren, Xinling
AU  - Ren X
AD  - Department of Respiratory Diseases, Xi'an, China.
FAU - Ma, Jin
AU  - Ma J
AD  - Department of Aerospace Physiology, Fourth Military Medical University, Xi'an, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201123
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Altitude Sickness/*complications/therapy
MH  - Animals
MH  - Cells, Cultured
MH  - Humans
MH  - Hypertension, Pulmonary/etiology/*therapy
MH  - Male
MH  - Muscle, Smooth, Vascular/cytology
MH  - Myocytes, Smooth Muscle/metabolism
MH  - Oxidative Stress
MH  - Oxygen/metabolism
MH  - Oxygen Inhalation Therapy/*methods
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transcriptome
OTO - NOTNLM
OT  - artery remodeling
OT  - high-altitude pulmonary hypertension
OT  - human pulmonary artery smooth muscle cells
OT  - intermittent reoxygenation
EDAT- 2020/11/25 06:00
MHDA- 2021/06/25 06:00
CRDT- 2020/11/24 06:11
PHST- 2020/03/06 00:00 [received]
PHST- 2020/11/04 00:00 [revised]
PHST- 2020/11/09 00:00 [accepted]
PHST- 2020/11/25 06:00 [pubmed]
PHST- 2021/06/25 06:00 [medline]
PHST- 2020/11/24 06:11 [entrez]
AID - 10.1096/fj.202000533RR [doi]
PST - ppublish
SO  - FASEB J. 2021 Feb;35(2):e21212. doi: 10.1096/fj.202000533RR. Epub 2020 Nov 23.