PMID- 33234730 OWN - NLM STAT- MEDLINE DCOM- 20210526 LR - 20221207 IS - 1945-4589 (Electronic) IS - 1945-4589 (Linking) VI - 12 IP - 24 DP - 2020 Nov 25 TI - Periodontitis in elderly patients with type 2 diabetes mellitus: impact on gut microbiota and systemic inflammation. PG - 25956-25980 LID - 10.18632/aging.202174 [doi] AB - Elderly patients with type 2 diabetes mellitus (T2DM) exhibit considerable periodontitis frequency, which causes tooth loss and poor quality of life. To investigate the impact of periodontitis on gut microbiota, we used 16S rRNA amplicon sequencing to characterize the composition and structure of gut microbiota among elderly patients with T2DM and periodontitis (T2DM_P), elderly patients with T2DM alone (T2DM_NP), and healthy volunteers. We identified 34 key gut microbiota markers that distinguished participants with different periodontal conditions and investigated their connections to other gut bacteria, as well as their clinical correlates. The most striking differences in co-occurrence networks between the T2DM_P and T2DM_NP groups comprised interactions involving dominant genera in the oral cavity (i.e., Streptococcus and Veillonella). Of the 34 identified key gut microbiota markers that distinguished participants with different periodontal conditions, 25 taxa were correlated with duration of diabetes, dry mouth or the peripheral levels of pro-inflammatory cytokines (e.g., tumor necrosis factor-alpha, interferon-gamma, prostaglandin E2, interleukin-17, and interleukin-6) and metabolic parameters (e.g., hemoglobin A1c), respectively. Our findings suggest that gut microbial shifts driven by periodontitis may contribute to systemic inflammation and metabolic dysfunction during the progression of T2DM. FAU - Li, Jinyou AU - Li J AD - Zhejiang Provincial Key Laboratory for Diagnosis and Treatment of Aging and Physic-Chemical Injury Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Lu, Haifeng AU - Lu H AD - State Key Laboratory for Diagnosis and Treatment of Infectious Disease, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Wu, Huanwen AU - Wu H AD - Deanery of Biomedical Sciences, The University of Edinburgh, Edinburgh, UK. FAU - Huang, Shunmei AU - Huang S AD - Zhejiang Provincial Key Laboratory for Diagnosis and Treatment of Aging and Physic-Chemical Injury Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Chen, Lufang AU - Chen L AD - Zhejiang Provincial Key Laboratory for Diagnosis and Treatment of Aging and Physic-Chemical Injury Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Gui, Qifeng AU - Gui Q AD - Zhejiang Provincial Key Laboratory for Diagnosis and Treatment of Aging and Physic-Chemical Injury Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Zhou, Wenjing AU - Zhou W AD - Zhejiang Provincial Key Laboratory for Diagnosis and Treatment of Aging and Physic-Chemical Injury Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Yang, Yichen AU - Yang Y AD - Zhejiang Provincial Key Laboratory for Diagnosis and Treatment of Aging and Physic-Chemical Injury Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Wu, Yue AU - Wu Y AD - Zhejiang Provincial Key Laboratory for Diagnosis and Treatment of Aging and Physic-Chemical Injury Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Zhang, Hua AU - Zhang H AD - State Key Laboratory for Diagnosis and Treatment of Infectious Disease, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Zhang, Qin AU - Zhang Q AD - Zhejiang Provincial Key Laboratory for Diagnosis and Treatment of Aging and Physic-Chemical Injury Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Yang, Yunmei AU - Yang Y AD - Zhejiang Provincial Key Laboratory for Diagnosis and Treatment of Aging and Physic-Chemical Injury Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20201125 PL - United States TA - Aging (Albany NY) JT - Aging JID - 101508617 RN - 0 (Glycated Hemoglobin A) RN - 0 (Interleukin-17) RN - 0 (Interleukin-6) RN - 0 (TNF protein, human) RN - 0 (Tumor Necrosis Factor-alpha) RN - 0 (hemoglobin A1c protein, human) RN - 82115-62-6 (Interferon-gamma) RN - K7Q1JQR04M (Dinoprostone) SB - IM MH - Aged MH - Aged, 80 and over MH - Case-Control Studies MH - Diabetes Mellitus, Type 2/*metabolism/microbiology MH - Dinoprostone/metabolism MH - Dysbiosis/*metabolism/microbiology MH - Female MH - *Gastrointestinal Microbiome MH - Glycated Hemoglobin/metabolism MH - Humans MH - Inflammation/*metabolism/microbiology MH - Interferon-gamma/metabolism MH - Interleukin-17/metabolism MH - Interleukin-6/metabolism MH - Male MH - Microbiota MH - Mouth/microbiology MH - Periodontitis/*metabolism/microbiology MH - Tumor Necrosis Factor-alpha/metabolism PMC - PMC7803515 OTO - NOTNLM OT - diabetes OT - gut microbiota OT - older individuals OT - periodontitis OT - systemic inflammation COIS- CONFLICTS OF INTEREST: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2020/11/26 06:00 MHDA- 2021/05/27 06:00 PMCR- 2020/12/31 CRDT- 2020/11/25 05:38 PHST- 2020/08/21 00:00 [received] PHST- 2020/09/29 00:00 [accepted] PHST- 2020/11/26 06:00 [pubmed] PHST- 2021/05/27 06:00 [medline] PHST- 2020/11/25 05:38 [entrez] PHST- 2020/12/31 00:00 [pmc-release] AID - 202174 [pii] AID - 10.18632/aging.202174 [doi] PST - ppublish SO - Aging (Albany NY). 2020 Nov 25;12(24):25956-25980. doi: 10.18632/aging.202174. Epub 2020 Nov 25.