PMID- 33235495
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220418
IS  - 1179-1322 (Print)
IS  - 1179-1322 (Electronic)
IS  - 1179-1322 (Linking)
VI  - 12
DP  - 2020
TI  - LncRNA-IUR Sponges miR-24 to Upregulate P53 in Laryngeal Squamous Cell Carcinoma.
PG  - 11639-11647
LID - 10.2147/CMAR.S236188 [doi]
AB  - OBJECTIVE: The functions of lncRNA-IUR in laryngeal squamous cell carcinoma 
      (LSCC) were investigated in this study. METHODS: RT-qPCR and paired t-test were 
      used to measure and compare expression levels of IUR, miR-24 and p53 in LSCC and 
      non-tumor tissues. Human LSCC cell line UM-SCC-17A was used and transfected by 
      pcDNA3.1 vector to overexpress IUR and miR-24. The transwell assay and wound 
      healing assay illustrated the effect of overexpression of IUR or miR-24 in the 
      cell invasion and migration of LSCC. Subcutaneous tumor model in nude mice was 
      carried out to demonstrate the mechanism between IUR and miR-24 in regulating 
      tumor growth. RESULTS: We found that IUR was downregulated in LSCC. Low 
      expression levels of IUR were correlated with the poor survival of LSCC patients. 
      Overexpression experiments showed that overexpression of IUR led to increased, 
      while overexpression of miR-24 led to decreased expression levels of p53 in LSCC 
      cells. And bioinformatics analysis showed that IUR may sponge miR-24. Cell 
      proliferation assay showed that overexpression of IUR and p53 led to decreased 
      proliferation rate of LSCC cells, while overexpression of miR-24 led to increased 
      proliferation rate of LSCC cells. We also illustrated that overexpression of IUR 
      promoted cell migration and invasion while miR-24 had opposite effects. In 
      addition, subcutaneous tumor model in nude mice showed that overexpression of 
      miR-24 attenuated the effects of overexpression of IUR on the expression of p53 
      and cancer cell proliferation. CONCLUSION: IUR sponges miR-24 to upregulate p53 
      in LSCC, thereby inhibiting cancer cell proliferation.
CI  - (c) 2020 Wei et al.
FAU - Wei, Cen
AU  - Wei C
AD  - ENT & HN Surgery Department, Wuming Hospital of Guangxi Medical University, 
      Nanning 530199, People's Republic of China.
FAU - Wei, Huaqing
AU  - Wei H
AD  - Department of Cardiology, Wuming Hospital of Guangxi Medical University, Nanning 
      530199, People's Republic of China.
FAU - Wu, Xun
AU  - Wu X
AD  - Department of Maxillofacial Surgery, Guangxi Medical University College of 
      Stomatology, Nanning 530021, People's Republic of China.
FAU - Nong, Guangyao
AU  - Nong G
AD  - ENT & HN Surgery Department, Wuming Hospital of Guangxi Medical University, 
      Nanning 530199, People's Republic of China.
FAU - Wu, Chenglin
AU  - Wu C
AD  - Department of ENT, Sixth Affiliated Hospital of Guangxi Medical University, 
      Nanning 537000, People's Republic of China.
FAU - Lee, Jinli
AU  - Lee J
AD  - Department of Gastroenterology, 923 Hospital of the Joint Logistics Support Force 
      of the Chinese People's Liberation Army, Nanning 530021, People's Republic of 
      China.
FAU - Meng, Ning
AU  - Meng N
AD  - Department of Maxillofacial Surgery, Guangxi Medical University College of 
      Stomatology, Nanning 530021, People's Republic of China.
FAU - Yu, Dahai
AU  - Yu D
AD  - Department of Stomatology, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning 530021, People's Republic of China.
FAU - Su, Jiping
AU  - Su J
AD  - ENT & HN Surgery Department, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning 530199, People's Republic of China.
FAU - Guo, Mengzhu
AU  - Guo M
AD  - Department of Stomatology, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning 530021, People's Republic of China.
FAU - Qin, Jiangyuan
AU  - Qin J
AD  - Department of Otolaryngology, Guangxi General Hospital of Chinses People's Armed 
      Police Force, Nanning 530000, People's Republic of China.
FAU - Fan, Xuemin
AU  - Fan X
AD  - Department of Maxillofacial Surgery, Guangxi Medical University College of 
      Stomatology, Nanning 530021, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20201116
PL  - New Zealand
TA  - Cancer Manag Res
JT  - Cancer management and research
JID - 101512700
PMC - PMC7678708
OTO - NOTNLM
OT  - IUR
OT  - laryngeal squamous cell carcinoma
OT  - miR-24
OT  - p53
COIS- The authors declare that they have no competing interests.
EDAT- 2020/11/26 06:00
MHDA- 2020/11/26 06:01
PMCR- 2020/11/16
CRDT- 2020/11/25 05:45
PHST- 2019/10/26 00:00 [received]
PHST- 2020/09/16 00:00 [accepted]
PHST- 2020/11/25 05:45 [entrez]
PHST- 2020/11/26 06:00 [pubmed]
PHST- 2020/11/26 06:01 [medline]
PHST- 2020/11/16 00:00 [pmc-release]
AID - 236188 [pii]
AID - 10.2147/CMAR.S236188 [doi]
PST - epublish
SO  - Cancer Manag Res. 2020 Nov 16;12:11639-11647. doi: 10.2147/CMAR.S236188. 
      eCollection 2020.