PMID- 33236326
OWN - NLM
STAT- MEDLINE
DCOM- 20211027
LR  - 20220531
IS  - 1559-1182 (Electronic)
IS  - 0893-7648 (Print)
IS  - 0893-7648 (Linking)
VI  - 58
IP  - 4
DP  - 2021 Apr
TI  - Increased Response to 3,4-Methylenedioxymethamphetamine (MDMA) Reward and Altered 
      Gene Expression in Zebrafish During Short- and Long-Term Nicotine Withdrawal.
PG  - 1650-1663
LID - 10.1007/s12035-020-02225-5 [doi]
AB  - An interactive effect between nicotine and 3,4-methylenedioxymethamphetamine 
      (MDMA) has been reported but the mechanism underlying such interaction is not 
      completely understood. This study used zebrafish to explore gene expression 
      changes associated with altered sensitivity to the rewarding effects of MDMA 
      following 2-week exposure to nicotine and 2-60 days of nicotine withdrawal. 
      Reward responses to MDMA were assessed using a conditioned place preference (CPP) 
      paradigm and gene expression was evaluated using quantitative real-time PCR of 
      mRNA from whole brain samples from drug-treated and control adult zebrafish. 
      Zebrafish pre-exposed for 2 weeks to nicotine showed increased conditioned place 
      preference in response to low-dose, 0.1 mg/kg, MDMA compared to un-exposed fish 
      at 2, 7, 30 and 60 days withdrawal. Pre-exposure to nicotine for 2 weeks induced 
      a significant increase of c-Fos and vasopressin receptor expression but a 
      decrease of D(3) dopaminergic and oxytocin receptor expression at 2 days of 
      withdrawal. C-Fos mRNA increased also at 7, 30, 60 days of withdrawal. Nicotine 
      pre-exposed zebrafish submitted to MDMA-induced CPP showed an increase in 
      expression of p35 at day 2, alpha4 at day 30, vasopressin at day 7 and D(3) 
      dopaminergic receptor at day 7, 30 and 60. These gene alterations could account 
      for the altered sensitivity to the rewarding effects of MDMA in nicotine 
      pre-exposed fish, suggesting that zebrafish have an altered ability to modulate 
      behaviour as a function of reward during nicotine withdrawal.
FAU - Ponzoni, Luisa
AU  - Ponzoni L
AUID- ORCID: 0000-0002-0652-0766
AD  - Department of Medical Biotechnology and Translational Medicine, Universita degli 
      Studi di Milano, Milan, Italy.
FAU - Teh, Muy-Teck
AU  - Teh MT
AUID- ORCID: 0000-0002-7725-8355
AD  - Centre for Oral Immunobiology and Regenerative Medicine, Institute of Dentistry, 
      Barts & The London School of Medicine and Dentistry, Queen Mary University of 
      London, England, UK.
FAU - Torres-Perez, Jose V
AU  - Torres-Perez JV
AUID- ORCID: 0000-0001-6390-9690
AD  - School of Biological and Chemical Sciences, Queen Mary University of London, 
      London, UK.
FAU - Brennan, Caroline H
AU  - Brennan CH
AUID- ORCID: 0000-0002-4169-4083
AD  - School of Biological and Chemical Sciences, Queen Mary University of London, 
      London, UK.
FAU - Braida, Daniela
AU  - Braida D
AUID- ORCID: 0000-0002-5867-1902
AD  - Department of Medical Biotechnology and Translational Medicine, Universita degli 
      Studi di Milano, Milan, Italy.
FAU - Sala, Mariaelvina
AU  - Sala M
AUID- ORCID: 0000-0001-9310-958X
AD  - Neuroscience Institute, CNR, Via Vanvitelli 32, 20129, Milan, Italy. 
      mariaelvina.sala@unimi.it.
LA  - eng
GR  - U01 DA044400/DA/NIDA NIH HHS/United States
PT  - Journal Article
DEP - 20201124
PL  - United States
TA  - Mol Neurobiol
JT  - Molecular neurobiology
JID - 8900963
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Dopamine)
RN  - 0 (Receptors, Oxytocin)
RN  - 0 (Receptors, Purinergic P1)
RN  - 6M3C89ZY6R (Nicotine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Animals
MH  - Conditioning, Classical
MH  - Female
MH  - *Gene Expression Regulation/drug effects
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - Nicotine/*adverse effects
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Dopamine/genetics/metabolism
MH  - Receptors, Oxytocin/genetics/metabolism
MH  - Receptors, Purinergic P1/genetics/metabolism
MH  - *Reward
MH  - Substance Withdrawal Syndrome/*genetics
MH  - Swimming
MH  - Time Factors
MH  - Zebrafish/*genetics
PMC - PMC8170707
MID - NIHMS1697602
OTO - NOTNLM
OT  - Conditioned place preference
OT  - Psychostimulants
OT  - Receptors
OT  - Reward
COIS- Conflicts of interest The authors declare that they have no conflicts of 
      interest.
EDAT- 2020/11/26 06:00
MHDA- 2021/10/28 06:00
PMCR- 2021/06/02
CRDT- 2020/11/25 05:50
PHST- 2020/08/12 00:00 [received]
PHST- 2020/11/19 00:00 [accepted]
PHST- 2020/11/26 06:00 [pubmed]
PHST- 2021/10/28 06:00 [medline]
PHST- 2020/11/25 05:50 [entrez]
PHST- 2021/06/02 00:00 [pmc-release]
AID - 10.1007/s12035-020-02225-5 [pii]
AID - 10.1007/s12035-020-02225-5 [doi]
PST - ppublish
SO  - Mol Neurobiol. 2021 Apr;58(4):1650-1663. doi: 10.1007/s12035-020-02225-5. Epub 
      2020 Nov 24.