PMID- 33238163
OWN - NLM
STAT- MEDLINE
DCOM- 20220107
LR  - 20220107
IS  - 1879-0542 (Electronic)
IS  - 0165-2478 (Linking)
VI  - 229
DP  - 2021 Jan
TI  - Combining miR-23b exposure with mesenchymal stem cell transplantation enhances 
      therapeutic effects on EAE.
PG  - 18-26
LID - S0165-2478(20)30418-1 [pii]
LID - 10.1016/j.imlet.2020.11.007 [doi]
AB  - Bone marrow mesenchymal stem cells (BMSCs) have the immuno-modulatory capacity to 
      ameliorate autoimmune diseases, such as multiple schlerosis (MS), systemic lupus 
      erythematosus and rheumatoid arthritis. However, BMSC-mediated immunosuppression 
      can be challenging to achieve. The efficacy of BMSC transplantation may be 
      augmented by an adjuvant therapy. Here, we demonstrated that treatment of mice 
      with experimental autoimmune encephalomyelitis (EAE), a model of MS, with BMSCs 
      over-expressing microRNA (miR)-23b provided better synergistic and longer-term 
      therapeutic effects than treatment with traditional BMSCs. Over-expression of 
      miR-23b enhanced the ability of BMSCs to inhibit differentiation of Th17 cells 
      and reduced IL-17 secretion. Compared to traditional BMSCs, the miR-23b 
      over-expressing BMSCs (miR23b-BMSCs) exhibited enhanced secretion of tumor growth 
      factor beta 1 (TGF-beta(1)), a cytokine that promotes the differentiation of 
      regulatory T (Treg) cells. Pathologically, miR23b-BMSC transplantation delayed 
      EAE progression, apparently by reducing the Th17/Treg cell ratio and inhibiting 
      inflammatory cell infiltration across the blood-brain barrier, and thus slowing 
      spinal cord demyelination. These results may lead to better utility of BMSCs as a 
      treatment for autoimmune diseases.
CI  - Copyright (c) 2020 European Federation of Immunological Societies. Published by 
      Elsevier B.V. All rights reserved.
FAU - Hu, Ruixue
AU  - Hu R
AD  - Department of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 
      150081, China.
FAU - Lv, Weiqi
AU  - Lv W
AD  - Department of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 
      150081, China.
FAU - Zhang, Sifan
AU  - Zhang S
AD  - Department of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 
      150081, China.
FAU - Liu, Yumei
AU  - Liu Y
AD  - Department of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 
      150081, China.
FAU - Sun, Bo
AU  - Sun B
AD  - Department of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 
      150081, China.
FAU - Meng, Yanting
AU  - Meng Y
AD  - Department of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 
      150081, China.
FAU - Kong, Qingfei
AU  - Kong Q
AD  - Department of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 
      150081, China.
FAU - Mu, Lili
AU  - Mu L
AD  - Department of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 
      150081, China.
FAU - Wang, Guangyou
AU  - Wang G
AD  - Department of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 
      150081, China.
FAU - Zhang, Yao
AU  - Zhang Y
AD  - Department of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 
      150081, China.
FAU - Li, Hulun
AU  - Li H
AD  - Department of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 
      150081, China.
FAU - Liu, Xijun
AU  - Liu X
AD  - Department of Neurobiology, Harbin Medical University, Harbin, Heilongjiang, 
      150081, China. Electronic address: lxj@hrbmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201122
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn23b microRNA, mouse)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Cell Line
MH  - Cytokines/metabolism
MH  - Disease Management
MH  - Disease Models, Animal
MH  - Disease Susceptibility
MH  - Encephalomyelitis, Autoimmune, Experimental/diagnosis/*etiology/*therapy
MH  - Gene Expression
MH  - Genetic Vectors/genetics
MH  - Humans
MH  - Immunophenotyping
MH  - Inflammation Mediators/metabolism
MH  - *Mesenchymal Stem Cell Transplantation/methods
MH  - Mesenchymal Stem Cells/*cytology/*metabolism
MH  - Mice
MH  - MicroRNAs/*genetics
MH  - Signal Transduction
MH  - Spinal Cord/immunology/metabolism/pathology
MH  - T-Lymphocytes/immunology/metabolism
MH  - Transduction, Genetic
MH  - Treatment Outcome
OTO - NOTNLM
OT  - Bone mesenchymal stem cells
OT  - Experimental autoimmune encephalomyelitis
OT  - Lentivirus packaging system
OT  - MiR-23b
OT  - Multiple sclerosis
EDAT- 2020/11/26 06:00
MHDA- 2022/01/08 06:00
CRDT- 2020/11/25 20:05
PHST- 2020/09/02 00:00 [received]
PHST- 2020/11/01 00:00 [revised]
PHST- 2020/11/18 00:00 [accepted]
PHST- 2020/11/26 06:00 [pubmed]
PHST- 2022/01/08 06:00 [medline]
PHST- 2020/11/25 20:05 [entrez]
AID - S0165-2478(20)30418-1 [pii]
AID - 10.1016/j.imlet.2020.11.007 [doi]
PST - ppublish
SO  - Immunol Lett. 2021 Jan;229:18-26. doi: 10.1016/j.imlet.2020.11.007. Epub 2020 Nov 
      22.