PMID- 33238581
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201201
IS  - 2072-6694 (Print)
IS  - 2072-6694 (Electronic)
IS  - 2072-6694 (Linking)
VI  - 12
IP  - 11
DP  - 2020 Nov 23
TI  - Calcitriol in the Presence of Conditioned Media from Metastatic Breast Cancer 
      Cells Enhances Ex Vivo Polarization of M2 Alternative Murine Bone Marrow-Derived 
      Macrophages.
LID - 10.3390/cancers12113485 [doi]
LID - 3485
AB  - In this study, we differentiated murine bone marrow-derived macrophages (BMDMs) 
      into M0, M1, and M2 in the presence or absence of calcitriol. Real-time PCR 
      analysis of gene expression, FACS analysis of surface markers, and 
      chemokine/cytokine production assays were performed. In addition, the effect of 
      the conditioned media (CM) from murine breast cancer 4T1 (metastatic) and 67NR 
      (non-metastatic) and Eph4-Ev (normal) cells with and without calcitriol on the 
      polarization of M1/M2 cells was determined. We found that calcitriol enhanced the 
      differentiation of M2 macrophages, which was manifested by increased expression 
      of Cd206 and Spp1 mRNA and CD36, Arg, and CCL2 in M2 BMDMs and by decreased 
      expression of Cd80 and Spp1 mRNA and IL-1, IL-6, OPN, and iNOS in M1 BMDMs. 4T1 
      CM showed a higher effect on the gene and protein expression in macrophages than 
      67NR and Eph4-Ev, with the greatest effect observed on M2 macrophages which 
      increased their differentiation and properties characteristic of alternative 
      macrophages. Moreover, M2 macrophages differentiated with calcitriol-stimulated 
      migration of 4T1 and 67NR cells through fibronectin and collagen type IV, 
      respectively. Overall, our results indicated that vitamin D supplementation may 
      not always be beneficial, especially in relation to cancers causing excessive, 
      pathological activation of the immune system.
FAU - Anisiewicz, Artur
AU  - Anisiewicz A
AUID- ORCID: 0000-0001-8412-6599
AD  - Department of Experimental Oncology, Hirszfeld Institute of Immunology and 
      Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
FAU - Labedz, Natalia
AU  - Labedz N
AUID- ORCID: 0000-0003-3320-0415
AD  - Department of Experimental Oncology, Hirszfeld Institute of Immunology and 
      Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
FAU - Krauze, Izabela
AU  - Krauze I
AD  - Department of Experimental Oncology, Hirszfeld Institute of Immunology and 
      Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
FAU - Wietrzyk, Joanna
AU  - Wietrzyk J
AUID- ORCID: 0000-0003-4980-6606
AD  - Department of Experimental Oncology, Hirszfeld Institute of Immunology and 
      Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
LA  - eng
GR  - DEC-2017/25/N/NZ5/02474/National Science Center, Poland/
PT  - Journal Article
DEP - 20201123
PL  - Switzerland
TA  - Cancers (Basel)
JT  - Cancers
JID - 101526829
PMC - PMC7700498
OTO - NOTNLM
OT  - 4T1 cells
OT  - 67NR cells
OT  - BMDMs
OT  - M2 macrophages
OT  - breast cancer cells
OT  - calcitriol
OT  - macrophages
OT  - vitamin D
COIS- The authors declare no conflict of interest.
EDAT- 2020/11/27 06:00
MHDA- 2020/11/27 06:01
PMCR- 2020/11/23
CRDT- 2020/11/26 01:01
PHST- 2020/11/09 00:00 [received]
PHST- 2020/11/17 00:00 [revised]
PHST- 2020/11/19 00:00 [accepted]
PHST- 2020/11/26 01:01 [entrez]
PHST- 2020/11/27 06:00 [pubmed]
PHST- 2020/11/27 06:01 [medline]
PHST- 2020/11/23 00:00 [pmc-release]
AID - cancers12113485 [pii]
AID - cancers-12-03485 [pii]
AID - 10.3390/cancers12113485 [doi]
PST - epublish
SO  - Cancers (Basel). 2020 Nov 23;12(11):3485. doi: 10.3390/cancers12113485.