PMID- 33242451
OWN - NLM
STAT- MEDLINE
DCOM- 20210427
LR  - 20210427
IS  - 1096-0945 (Electronic)
IS  - 0014-4800 (Linking)
VI  - 118
DP  - 2021 Feb
TI  - Analysis of latent T-cell epitopes in Epstein-Barr virus isolated from extranodal 
      nasal-type natural killer/T-cell lymphoma in Taiwanese population.
PG  - 104577
LID - S0014-4800(20)30854-6 [pii]
LID - 10.1016/j.yexmp.2020.104577 [doi]
AB  - Extranodal nasal-type natural killer (NK)/T-cell lymphoma (NKTCL) is an 
      aggressive lymphoma that is prevalent among East Asian and South American 
      populations. Although Epstein-Barr virus (EBV) is commonly detected in NKTCL, 
      there are limited studies that have analyzed the EBV genomic variations in NKTCL. 
      In this study, 8 EBV latent genes were analyzed using targeted gene sequencing in 
      23 formalin-fixed paraffin-embedded tissues derived from 18 patients with NKTCL. 
      Five cases with paired samples were comparatively analyzed. The consistency of 
      EBV sequencing data between tissue samples was high (96.3%-98.7%), whereas that 
      of variant calling among the tissue samples and plasma samples (74.3%-79.2%) was 
      low. The highest densities of non-synonymous variants were detected in the EBNA3B 
      gene. Among the 74 known T-cell epitopes, 363 non-synonymous variants were 
      identified in 32 (43.2%) epitopes. Additionally, the AVFDRKSDAK (A1S/P and 
      V2F/M/L) and YHLIVDTDSL (I4L and L10R/V/G/H) epitopes were associated with 5 
      patterns of amino acid changes in EBNA3B and EBNA-2, respectively. The frequency 
      of variation in the human leukocyte antigen (HLA)-restricted epitopes with 
      corresponding HLA types common among Taiwanese population was significantly low 
      (P = 0.011), whereas that in anchor residues was significantly high (P = 0.012). 
      In conclusion, this study demonstrated the genomic diversity of EBV in NKTCL and 
      its correlation with the HLA-restricted epitope variations in Taiwanese 
      population. The findings of this study provide useful insights for the 
      development of novel therapeutic strategies for NKTCL.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Chou, Chih-Chi
AU  - Chou CC
AD  - Department of Laboratory Medicine, Kaohsiung Chang Gung Memorial Hospital and 
      Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan; Department of 
      Pathology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University 
      College of Medicine, Kaohsiung 833031, Taiwan. Electronic address: 
      d22135@cgmh.org.tw.
FAU - Tsao, Cheng-Feng
AU  - Tsao CF
AD  - Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang 
      Gung University College of Medicine, Kaohsiung 83301, Taiwan. Electronic address: 
      a9650@cgmh.org.tw.
FAU - Liao, Chun-Kai
AU  - Liao CK
AD  - Department of Internal Medicine, Hematology-Oncology Division, Kaohsiung Chang 
      Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 
      83301, Taiwan.
FAU - You, Huey-Ling
AU  - You HL
AD  - Department of Laboratory Medicine, Kaohsiung Chang Gung Memorial Hospital and 
      Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan; Department of 
      Medical Laboratory Sciences and Biotechnology, Fooyin University, Kaohsiung 
      83102, Taiwan. Electronic address: ouhling@cgmh.org.tw.
FAU - Wang, Ming-Chung
AU  - Wang MC
AD  - Department of Internal Medicine, Hematology-Oncology Division, Kaohsiung Chang 
      Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 
      83301, Taiwan. Electronic address: wangmt@cgmh.org.tw.
FAU - Huang, Wan-Ting
AU  - Huang WT
AD  - Department of Laboratory Medicine, Kaohsiung Chang Gung Memorial Hospital and 
      Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan; Department of 
      Pathology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University 
      College of Medicine, Kaohsiung 833031, Taiwan; Department of Medical Laboratory 
      Sciences and Biotechnology, Fooyin University, Kaohsiung 83102, Taiwan. 
      Electronic address: huangminnie@cgmh.org.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201123
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Epstein-Barr Virus Nuclear Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Epitopes, T-Lymphocyte/*immunology
MH  - Epstein-Barr Virus Infections/*complications/virology
MH  - Epstein-Barr Virus Nuclear Antigens/*genetics
MH  - Female
MH  - Follow-Up Studies
MH  - Genome, Viral
MH  - Herpesvirus 4, Human/*physiology
MH  - Histocompatibility Antigens Class I/immunology
MH  - Humans
MH  - Killer Cells, Natural
MH  - Lymphoma, Extranodal NK-T-Cell/immunology/*pathology/virology
MH  - Male
MH  - Middle Aged
MH  - *Polymorphism, Single Nucleotide
MH  - Prognosis
MH  - Virus Latency/genetics/*immunology
MH  - Young Adult
OTO - NOTNLM
OT  - Epstein-Barr virus
OT  - Extranodal nasal-type natural killer/T-cell lymphoma
OT  - Latent genes
EDAT- 2020/11/27 06:00
MHDA- 2021/04/28 06:00
CRDT- 2020/11/26 20:07
PHST- 2020/05/28 00:00 [received]
PHST- 2020/08/18 00:00 [revised]
PHST- 2020/11/09 00:00 [accepted]
PHST- 2020/11/27 06:00 [pubmed]
PHST- 2021/04/28 06:00 [medline]
PHST- 2020/11/26 20:07 [entrez]
AID - S0014-4800(20)30854-6 [pii]
AID - 10.1016/j.yexmp.2020.104577 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2021 Feb;118:104577. doi: 10.1016/j.yexmp.2020.104577. Epub 2020 
      Nov 23.