PMID- 33242543
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210605
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 452
DP  - 2021 Jan 1
TI  - Sex- and Brain Region-specific Changes in Gene Expression in Male and Female Rats 
      as Consequences of Methamphetamine Self-administration and Abstinence.
PG  - 265-279
LID - S0306-4522(20)30742-9 [pii]
LID - 10.1016/j.neuroscience.2020.11.025 [doi]
AB  - Sex differences in METH use exist among human METH users and in animal models of 
      METH addiction. Herein, we tried to identify potential differences in gene 
      expression between female and male rats after Methamphetamine self-administration 
      (METH SA). Rats were trained to self-administer METH using two 3-hours daily 
      sessions for 20 days. Cue-induced drug seeking was measured on withdrawal days 3 
      (WD3) and 30 (WD30). Rats were euthanized twenty-four hours after WD30. 
      Prefrontal cortex (PFC) and hippocampus (HIP) were dissected to measure mRNA 
      expression. Both female and male rats increased their METH intake and showed 
      increased METH seeking during withdrawal. Female had higher basal level 
      expression of hypocretin receptor 1 (Hcrtr1) and prodynorphin (Pdyn) mRNAs in the 
      PFC and HIP. Basal corticotropin releasing hormone receptor 1 (Crhr1), Crh 
      receptor 2 (Crhr2), hypocretin receptor 2 (Hcrtr2) and opioid receptor kappa 1 
      (Oprk1) mRNA levels were higher in the PFC of females. Male rats had higher basal 
      levels of Crh and Crhr1 in HIP. METH SA was associated with increased Crh and 
      Crhr1 in the HIP of both sexes and Crhr2 only in female HIP. Importantly, 
      increased Crh and Crhr1 mRNA levels correlated positively with incubation of METH 
      craving in both sexes, supporting their potential involvement, in part, in the 
      regulation of this behavioral phenomenon. When taken together, our results 
      identified sexual dimorphic baseline differences in rats. We also detected 
      dimorphic responses in animals that had self-administered METH. These 
      observations highlight the importance of understanding the molecular neurobiology 
      of sex differences when therapeutic interventions are planned against METH 
      addiction.
CI  - Published by Elsevier Ltd.
FAU - Daiwile, Atul P
AU  - Daiwile AP
AD  - Molecular Neuropsychiatry Research Branch, NIDA Intramural Research Program, 
      Baltimore, MD 21224, United States.
FAU - Jayanthi, Subramaniam
AU  - Jayanthi S
AD  - Molecular Neuropsychiatry Research Branch, NIDA Intramural Research Program, 
      Baltimore, MD 21224, United States.
FAU - Cadet, Jean Lud
AU  - Cadet JL
AD  - Molecular Neuropsychiatry Research Branch, NIDA Intramural Research Program, 
      Baltimore, MD 21224, United States. Electronic address: 
      jcadet@intra.nida.nih.gov.
LA  - eng
GR  - ZIA DA000552/ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20201124
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Central Nervous System Stimulants)
RN  - 44RAL3456C (Methamphetamine)
SB  - IM
MH  - Animals
MH  - *Central Nervous System Stimulants
MH  - Craving
MH  - Drug-Seeking Behavior
MH  - Female
MH  - Gene Expression
MH  - Male
MH  - *Methamphetamine
MH  - Rats
MH  - Self Administration
PMC - PMC8175033
MID - NIHMS1705565
OTO - NOTNLM
OT  - Methamphetamine self-administration
OT  - addiction
OT  - hippocampus
OT  - prefrontal cortex
OT  - sexual dimorphism
COIS- CONFLICT OF INTEREST The authors declare that they have no conflict of interest.
EDAT- 2020/11/27 06:00
MHDA- 2021/05/15 06:00
PMCR- 2021/06/03
CRDT- 2020/11/26 20:08
PHST- 2020/08/17 00:00 [received]
PHST- 2020/11/04 00:00 [revised]
PHST- 2020/11/15 00:00 [accepted]
PHST- 2020/11/27 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/11/26 20:08 [entrez]
PHST- 2021/06/03 00:00 [pmc-release]
AID - S0306-4522(20)30742-9 [pii]
AID - 10.1016/j.neuroscience.2020.11.025 [doi]
PST - ppublish
SO  - Neuroscience. 2021 Jan 1;452:265-279. doi: 10.1016/j.neuroscience.2020.11.025. 
      Epub 2020 Nov 24.