PMID- 33242555
OWN - NLM
STAT- MEDLINE
DCOM- 20210114
LR  - 20210114
IS  - 1096-0333 (Electronic)
IS  - 0041-008X (Linking)
VI  - 410
DP  - 2021 Jan 1
TI  - Andrographolide inhibits IL-1beta release in bone marrow-derived macrophages and 
      monocyte infiltration in mouse knee joints induced by monosodium urate.
PG  - 115341
LID - S0041-008X(20)30463-4 [pii]
LID - 10.1016/j.taap.2020.115341 [doi]
AB  - Andrographolide (AND) is the major diterpenoid in A. paniculata with wide 
      clinical application and has been shown to be a potent anti-inflammatory agent. 
      Gout is the leading inflammatory disease of the joints, and the deposition of 
      urate in the articular cavity attracts immune cells that release inflammatory 
      cytokines. Monosodium urate (MSU) is known to be one of the activators of the 
      NLRP3 (NLR family pyrin domain containing 3) inflammasome. After activation, the 
      NLRP3 inflammasome releases interleukin-1beta (IL-1beta), which causes the development 
      of many inflammatory diseases. The aim of the present study was to investigate 
      whether AND attenuates the release of IL-1beta mediated by the NLRP3 inflammasome. 
      The effects of AND were studied in bone marrow-derived macrophages (BMDMs) 
      treated with lipopolysaccharide (LPS) and MSU and in mice with MSU-induced joint 
      inflammation. AND suppressed MSU phagocytosis dose-dependently and markedly 
      inhibited LPS- and MSU-induced IL-1beta release in BMDMs. Moreover, AND pretreatment 
      inhibited the LPS-induced NLRP3 inflammasome priming stage by inhibiting the 
      IKK/NFkappaB signaling pathway, which resulted in decreased protein expression of 
      NLRP3 and proIL-1beta. AND induced HO-1 protein expression in a dose-dependent 
      manner and attenuated MSU-induced ROS generation. Silencing HO-1 mitigated AND 
      inhibition of LPS/MSU-induced IL-1beta release in J774A.1 cells. In addition, AND 
      decreased MSU-mediated ASC binding to NLRP3. Oral administration of AND 
      attenuated MSU-induced monocyte infiltration in mouse knee joints. These results 
      suggest that the working mechanisms by which AND down-regulates MSU-induced joint 
      inflammation might be via HO-1 induction and attenuation of ROS-mediated NLRP3 
      inflammasome assembly and subsequent IL-1beta release.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Lo, Chia-Wen
AU  - Lo CW
AD  - Department of Nutrition, China Medical University, Taichung, Taiwan.
FAU - Lii, Chong-Kuei
AU  - Lii CK
AD  - Department of Nutrition, China Medical University, Taichung, Taiwan; Department 
      of Food Nutrition and Health Biotechnology, Asia University, Taichung, Taiwan.
FAU - Hong, Jian-Jhe
AU  - Hong JJ
AD  - Department of Nutrition, China Medical University, Taichung, Taiwan.
FAU - Chuang, Wei-Ting
AU  - Chuang WT
AD  - Department of Nutrition, China Medical University, Taichung, Taiwan.
FAU - Yang, Ya-Chen
AU  - Yang YC
AD  - Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, 
      Taiwan.
FAU - Huang, Chin-Shiu
AU  - Huang CS
AD  - Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, 
      Taiwan.
FAU - Chen, Haw-Wen
AU  - Chen HW
AD  - Department of Nutrition, China Medical University, Taichung, Taiwan. Electronic 
      address: chenhw@mail.cmu.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201124
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Diterpenes)
RN  - 0 (IL1B protein, mouse)
RN  - 0 (Interleukin-1beta)
RN  - 268B43MJ25 (Uric Acid)
RN  - 410105JHGR (andrographolide)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Antioxidants/toxicity
MH  - Cell Line
MH  - Cells, Cultured
MH  - Diterpenes/*pharmacology
MH  - Humans
MH  - Interleukin-1beta/*antagonists & inhibitors/metabolism
MH  - Knee Joint/*drug effects/metabolism/pathology
MH  - Macrophages/*drug effects/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Monocytes/*drug effects/metabolism
MH  - Uric Acid/*toxicity
OTO - NOTNLM
OT  - Andrographolide (AND)
OT  - Bone marrow-derived macrophages (BMDMs)
OT  - Gout
OT  - Interleukin 1beta (IL-1beta)
OT  - Monosodium urate (MSU)
OT  - NLR family pyrin domain containing 3 (NLRP3) inflammasome
EDAT- 2020/11/27 06:00
MHDA- 2021/01/15 06:00
CRDT- 2020/11/26 20:08
PHST- 2020/08/03 00:00 [received]
PHST- 2020/11/12 00:00 [revised]
PHST- 2020/11/16 00:00 [accepted]
PHST- 2020/11/27 06:00 [pubmed]
PHST- 2021/01/15 06:00 [medline]
PHST- 2020/11/26 20:08 [entrez]
AID - S0041-008X(20)30463-4 [pii]
AID - 10.1016/j.taap.2020.115341 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 2021 Jan 1;410:115341. doi: 10.1016/j.taap.2020.115341. 
      Epub 2020 Nov 24.