PMID- 33244048
OWN - NLM
STAT- MEDLINE
DCOM- 20210315
LR  - 20210315
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Nov 26
TI  - A novel noninvasive approach for evaluating work of breathing indices in a 
      developmental rat model using respiratory inductance plethysmography.
PG  - 20730
LID - 10.1038/s41598-020-77731-6 [doi]
LID - 20730
AB  - Pulmonary function testing (PFT) is an important component for evaluating the 
      outcome of experimental rodent models of respiratory diseases. Respiratory 
      inductance plethysmography (RIP) provides a noninvasive method of PFT requiring 
      minimal cooperation. RIP measures work of breathing (WOB) indices including phase 
      angle (capital EF, Cyrillic), percent rib cage (RC %), breaths per minute (BPM), and labored 
      breathing index (LBI) on an iPad. The aim of this study was to evaluate the 
      utility of a recently developed research instrument, pneuRIP, for evaluation of 
      WOB indices in a developmental rat model. Sprague Dawley rats (2 months old) were 
      commercially acquired and anaesthetised with isoflurane. The pneuRIP system uses 
      two elastic bands: one band (RC) placed around the rib cage under the upper 
      armpit and another band (AB) around the abdomen. The typical thoracoabdominal 
      motion (TAM) plot showed the abdomen and rib cage motion in synchrony. The plots 
      of phase angle and LBI as a function of data point number showed that values were 
      within the range. The distribution for phase angle and LBI was within a narrow 
      range. pneuRIP testing provided instantaneous PFT results. This study 
      demonstrated the utility of RIP as a rapid, noninvasive approach for evaluating 
      treatment interventions in the rodent model.
FAU - Zhu, Yan
AU  - Zhu Y
AD  - Department of Biomedical Research and Center for Pediatric Lung Research, 
      Nemours/Alfred I. duPont Hospital for Children, P.O. Box 269, Wilmington, DE, 
      19803, USA.
FAU - Aghai, Zubair H
AU  - Aghai ZH
AD  - Department of Biomedical Research and Center for Pediatric Lung Research, 
      Nemours/Alfred I. duPont Hospital for Children, P.O. Box 269, Wilmington, DE, 
      19803, USA.
AD  - Pediatrics and Neonatology, Sidney Kimmel Medical College at Thomas Jefferson 
      University, Philadelphia, PA, USA.
FAU - Betal, Suhita Gayen Nee
AU  - Betal SGN
AD  - Department of Biomedical Research and Center for Pediatric Lung Research, 
      Nemours/Alfred I. duPont Hospital for Children, P.O. Box 269, Wilmington, DE, 
      19803, USA.
AD  - Pediatrics and Neonatology, Sidney Kimmel Medical College at Thomas Jefferson 
      University, Philadelphia, PA, USA.
FAU - Favara, Michael
AU  - Favara M
AD  - Pediatrics and Neonatology, Sidney Kimmel Medical College at Thomas Jefferson 
      University, Philadelphia, PA, USA.
FAU - Fong, Gina
AU  - Fong G
AD  - Pediatrics and Neonatology, Sidney Kimmel Medical College at Thomas Jefferson 
      University, Philadelphia, PA, USA.
AD  - Division of Neonatology, Nemours/Alfred I. duPont Hospital for Children, 
      Wilmington, DE, USA.
FAU - Rahman, Tariq
AU  - Rahman T
AD  - Department of Biomedical Research and Center for Pediatric Lung Research, 
      Nemours/Alfred I. duPont Hospital for Children, P.O. Box 269, Wilmington, DE, 
      19803, USA.
FAU - Shaffer, Thomas H
AU  - Shaffer TH
AD  - Department of Biomedical Research and Center for Pediatric Lung Research, 
      Nemours/Alfred I. duPont Hospital for Children, P.O. Box 269, Wilmington, DE, 
      19803, USA. thomas.shaffer@nemours.org.
AD  - Pediatrics and Neonatology, Sidney Kimmel Medical College at Thomas Jefferson 
      University, Philadelphia, PA, USA. thomas.shaffer@nemours.org.
LA  - eng
GR  - NIH COBRE P30GM114736/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20201126
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - CYS9AKD70P (Isoflurane)
SB  - IM
MH  - Abdomen/physiology
MH  - Animals
MH  - Child
MH  - Humans
MH  - Infant
MH  - Isoflurane/administration & dosage
MH  - Lung/drug effects/physiology
MH  - Models, Animal
MH  - Movement/drug effects/physiology
MH  - Plethysmography/*methods
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Respiration/drug effects
MH  - Respiratory Function Tests/methods
MH  - Respiratory Mechanics/drug effects/physiology
MH  - Work of Breathing/drug effects/*physiology
PMC - PMC7691344
COIS- The authors declare no competing interests.
EDAT- 2020/11/28 06:00
MHDA- 2021/03/16 06:00
PMCR- 2020/11/26
CRDT- 2020/11/27 05:41
PHST- 2020/05/27 00:00 [received]
PHST- 2020/10/26 00:00 [accepted]
PHST- 2020/11/27 05:41 [entrez]
PHST- 2020/11/28 06:00 [pubmed]
PHST- 2021/03/16 06:00 [medline]
PHST- 2020/11/26 00:00 [pmc-release]
AID - 10.1038/s41598-020-77731-6 [pii]
AID - 77731 [pii]
AID - 10.1038/s41598-020-77731-6 [doi]
PST - epublish
SO  - Sci Rep. 2020 Nov 26;10(1):20730. doi: 10.1038/s41598-020-77731-6.