PMID- 3324459
OWN - NLM
STAT- MEDLINE
DCOM- 19880302
LR  - 20190828
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 16
IP  - 3-4
DP  - 1987 Nov
TI  - Bovine leukocyte phagocytosis and bacteria killing monitored by intracellular 
      acridine orange fluorescence and extracellular fluorescence quenching.
PG  - 185-99
AB  - The time course of phagocytosis and intracellular killing of serum-opsonized 
      Escherichia coli K12 and Staphylococcus aureus SG511 by glass-adherent bovine 
      peripheral blood polymorphonuclear leukocytes (PMNLs) and cultured monocytes 
      (macrophages) was monitored by fluorescence microscopy of single cells using the 
      acridine orange (AO)/crystal violet (CV) technique. After interaction of 
      glass-adherent leukocytes (20, 40, 60 min, 37 degrees C) with opsonized bacteria, 
      cells were stained with the fluorescent dye AO. Living bacteria stained green, 
      dead bacteria stained orange. The addition of CV to AO-stained bacteria quenched 
      the fluorescence of extracellular bacteria only. CV does not penetrate living 
      bovine PMNLs which allows the discrimination of ingested (fluorescent) and 
      extracellular (nonfluorescent) bacteria during attachment and phagocytosis of 
      bacteria by adherent PMNLs. We investigated quantitatively phagocytosis and 
      intracellular killing of serum-opsonized bacteria by bovine PMNLs from 22 bulls 
      of 4 different Swiss dairy breeds. Within 60 min maximum uptake (approximately 12 
      bacteria/PMNL) and killing (approximately 80%) of serum-opsonized Escherichia 
      coli K12 and Staphylococcus aureus SG511 were achieved. The AO/CV technique was 
      also used to quantify the uptake and intracellular killing of serum-opsonized 
      Escherichia coli K12 by cultured monocytes (macrophages). Within 60 min maximum 
      uptake of bacteria (approximately 16/MO) was achieved; approximately 83% of 
      bacteria were killed.
FAU - Zanetti, M
AU  - Zanetti M
AD  - Institute of Animal Husbandry, University of Berne, Switzerland.
FAU - Schmitt, M
AU  - Schmitt M
FAU - Lazary, S
AU  - Lazary S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Opsonin Proteins)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Bacteria/*immunology
MH  - Cattle/*immunology
MH  - Cell Adhesion
MH  - Escherichia coli/immunology
MH  - Kinetics
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Monocytes/*immunology
MH  - Neutrophils/*immunology
MH  - Opsonin Proteins
MH  - *Phagocytosis
MH  - Staphylococcus aureus/immunology
EDAT- 1987/11/01 00:00
MHDA- 1987/11/01 00:01
CRDT- 1987/11/01 00:00
PHST- 1987/11/01 00:00 [pubmed]
PHST- 1987/11/01 00:01 [medline]
PHST- 1987/11/01 00:00 [entrez]
AID - 0165-2427(87)90017-1 [pii]
AID - 10.1016/0165-2427(87)90017-1 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 1987 Nov;16(3-4):185-99. doi: 
      10.1016/0165-2427(87)90017-1.