PMID- 33244658
OWN - NLM
STAT- MEDLINE
DCOM- 20210723
LR  - 20210723
IS  - 1436-2236 (Electronic)
IS  - 1436-2228 (Linking)
VI  - 22
IP  - 6
DP  - 2020 Dec
TI  - Characterization of Macroscopic Colony-Forming Filamentous Cyanobacteria from 
      Okinawan Coasts as Potential Sources of Bioactive Compounds.
PG  - 824-835
LID - 10.1007/s10126-020-10010-7 [doi]
AB  - Marine macroscopic colony-forming filamentous (MMCFF) cyanobacteria are 
      considered as prolific producers of bioactive compounds. Thus, knowledge of the 
      diversity of MMCFF cyanobacteria as related to bioactive compound production has 
      become very important. However, basic taxonomic studies of MMCFF cyanobacteria 
      are lacking. Many cyanobacterial taxa are still misidentified or undescribed. In 
      this study, a total of 32 cyanobacterial colonies from nine coastal regions of 
      Okinawa Prefecture were investigated for a diversity assessment. A polyphasic 
      approach including morphological and molecular studies based on 16S rRNA gene 
      sequences was performed to characterize Okinawan MMCFF cyanobacteria. Both 
      morphological and molecular phylogenetic results showed that MMCFF cyanobacteria 
      from Okinawan coasts are very diverse. We found morphotypes of Lyngbya-like, 
      Phormidium-like, and Leptolyngbya-like groups among Okinawan cyanobacterial 
      samples. Genetically, samples were distributed in various clades in the 
      phylogenetic tree, including within Moorena, Okeania, Caldora, Neolyngbya, Dapis, 
      as well as several unknown clades. In addition, cytotoxic activities of three 
      samples from Kiyan coast were tested against HeLa cells. All three crude extracts 
      of these samples showed strong cytotoxic activity with IC(50) < 1 mug/ml.
FAU - Nuryadi, Handung
AU  - Nuryadi H
AD  - Graduate School of Engineering and Science, University of the Ryukyus, Nishihara, 
      Okinawa, Japan.
FAU - Sumimoto, Shimpei
AU  - Sumimoto S
AD  - Department of Material and Life Chemistry, Kanagawa University, Yokohama, 
      Kanagawa, Japan.
FAU - Teruya, Toshiaki
AU  - Teruya T
AD  - Faculty of Education, University of the Ryukyus, Nishihara, Okinawa, Japan.
FAU - Suenaga, Kiyotake
AU  - Suenaga K
AD  - Department of Chemistry, Keio University, Yokohama, Kanagawa, Japan.
FAU - Suda, Shoichiro
AU  - Suda S
AD  - Faculty of Science, University of the Ryukyus, Nishihara, Okinawa, Japan. 
      sudas@sci.u-ryukyu.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20201126
PL  - United States
TA  - Mar Biotechnol (NY)
JT  - Marine biotechnology (New York, N.Y.)
JID - 100892712
RN  - 0 (Complex Mixtures)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Complex Mixtures/toxicity
MH  - Cyanobacteria/*chemistry/*classification/genetics
MH  - HeLa Cells
MH  - Humans
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
OTO - NOTNLM
OT  - 16S rRNA phylogeny
OT  - Cytotoxic activity
OT  - Marine macroscopic colony-forming filamentous (MMCFF) cyanobacteria
OT  - Morphological characterization
EDAT- 2020/11/28 06:00
MHDA- 2021/07/24 06:00
CRDT- 2020/11/27 05:47
PHST- 2020/03/27 00:00 [received]
PHST- 2020/11/12 00:00 [accepted]
PHST- 2020/11/28 06:00 [pubmed]
PHST- 2021/07/24 06:00 [medline]
PHST- 2020/11/27 05:47 [entrez]
AID - 10.1007/s10126-020-10010-7 [pii]
AID - 10.1007/s10126-020-10010-7 [doi]
PST - ppublish
SO  - Mar Biotechnol (NY). 2020 Dec;22(6):824-835. doi: 10.1007/s10126-020-10010-7. 
      Epub 2020 Nov 26.