PMID- 33245099
OWN - NLM
STAT- MEDLINE
DCOM- 20211210
LR  - 20211214
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 41
IP  - 1
DP  - 2021 Jan 29
TI  - Ligustrazine inhibits the proliferation and migration of ovarian cancer cells via 
      regulating miR-211.
LID - 10.1042/BSR20200199 [doi]
LID - BSR20200199
AB  - Ovarian cancer (OC) is a commonly diagnosed female cancer. Ligustrazine (LSZ), a 
      natural compound, has been reported to exert anti-cancer activity, although the 
      mechanisms underlying the anti-cancer effects are not clear. The present study 
      investigated the impact of LSZ on cell proliferation and migration by regulating 
      microRNA-211 (miR-211) expression using the human ovarian cancer SK-OV-3 and 
      OVCAR-3 cell lines. OC cells were treated with 0, 0.5, 1, and 2 mM LSZ, and 
      quantitative real-time PCR was utilized to measure miR-211 levels in SK-OV-3 and 
      OVCAR-3 cells with different treatment. Moreover, to further confirm the roles of 
      miR-211 in LSZ induced anti-tumor effects, miR-211 expression was inhibited by 
      transfection of miR-211 inhibitors in SK-OV-3 cells. Cell proliferation of 
      transfected cells was evaluated using the CCK-8 and colony formation assay. The 
      scratch assay was employed to assess cell migration and transwell assay was 
      performed for evaluating the cell invasion. Protein levels of 
      epithelial-mesenchymal transition (EMT) markers were determined by Western 
      blotting. We found that LSZ inhibited the viability, proliferation, migration and 
      invasion ability of SK-OV-3 and OVCAR-3 cells in a dose-dependent manner; 
      moreover, LSZ could significantly increase the expression of miR-211 in both 
      SK-OV-3 and OVCAR-3, and knockdown of miR-211 in SK-OV-3 cells partially 
      abrogated the anti-tumor behavior of LSZ by promoting the viability, 
      proliferation, migration, invasion and EMT of SK-OV-3 cells. Thus, we found that 
      LSZ can inhibit the proliferation and migration of OC cells via regulating 
      miR-211. Our study suggests that LSZ might be a potential and effective treatment 
      for OC.
CI  - (c) 2021 The Author(s).
FAU - Zhang, Hairong
AU  - Zhang H
AD  - Department of Obstetrics and Gynecology, Shandong Provincial Third Hospital, 
      Cheeloo College of Medicine, Shandong University, Jinan 250031, China.
FAU - Ding, Shichao
AU  - Ding S
AD  - Department of Internal Medicine, The Third Affiliated Hospital of Shandong First 
      Medical University, Jinan 250031, China.
AD  - Department of Internal Medicine, Affiliated Hospital of Shandong Academy of 
      Medical Sciences, Jinan 250031, China.
FAU - Xia, Lei
AU  - Xia L
AD  - Department of Pathology, Shandong University of Traditional Chinese Medicine, 
      Jinan 250355, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (MIRN211 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Pyrazines)
RN  - V80F4IA5XG (tetramethylpyrazine)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Movement/*drug effects
MH  - Cell Proliferation/*drug effects
MH  - Cell Survival/drug effects
MH  - Epithelial-Mesenchymal Transition/drug effects
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Ovarian Neoplasms/genetics/*pathology
MH  - Pyrazines/*pharmacology
PMC - PMC7786329
OTO - NOTNLM
OT  - EMT
OT  - LSZ
OT  - SK-OV-3
OT  - miR-211
COIS- The authors declare that there are no competing interests associated with the 
      manuscript.
EDAT- 2020/11/28 06:00
MHDA- 2021/12/15 06:00
PMCR- 2021/01/05
CRDT- 2020/11/27 08:36
PHST- 2020/03/20 00:00 [received]
PHST- 2020/11/20 00:00 [revised]
PHST- 2020/11/24 00:00 [accepted]
PHST- 2020/11/28 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2020/11/27 08:36 [entrez]
PHST- 2021/01/05 00:00 [pmc-release]
AID - 227058 [pii]
AID - BSR20200199 [pii]
AID - 10.1042/BSR20200199 [doi]
PST - ppublish
SO  - Biosci Rep. 2021 Jan 29;41(1):BSR20200199. doi: 10.1042/BSR20200199.