PMID- 33247490
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240222
IS  - 1096-9888 (Electronic)
IS  - 1076-5174 (Linking)
VI  - 56
IP  - 4
DP  - 2020 Nov 20
TI  - Differential proteome analysis of the leaves of lead hyperaccumulator, Rhoeo 
      discolor (L. Her.) Hance.
PG  - e4689
LID - 10.1002/jms.4689 [doi]
AB  - The present study investigated Rhoeo discolor (L. Her.) Hance for its ability to 
      accumulate Pb, which is of relevance to phytoremediation applications. Based on 
      this analysis, plants were found to accumulate greater than 10 mg/g (0.1%) of dry 
      weight Pb in the shoots, which classifies the plant a Pb hyperaccumulator. 
      Further, changes in the leaf proteome profiles in response to Pb stress were 
      investigated. Wild-type plants were subjected to a high concentration of Pb(NO(3) 
      )(2) , and the levels of Pb that accumulated in different plant tissues were 
      determined using atomic absorption spectrophotometry. Using 2D-difference gel 
      electrophoresis, 181 protein spots were detected to be differentially abundant in 
      response to Pb stress and selected spots exhibiting the strongest differential 
      abundance suggested an impairment of the photosynthetic apparatus of the plant 
      under Pb stress. Subsequently, a more extensive, proteome wide analysis utilizing 
      label-free quantitation further identified a predominant decrease in protein 
      levels and a significant effect on the nuclear proteome, as well as 
      photosynthesis, carbon fixation and metabolism, providing insight into the Pb 
      tolerance of this system in a potential phytoremediation context.
CI  - (c) 2020 John Wiley & Sons, Ltd.
FAU - Enot, Melania M
AU  - Enot MM
AD  - Department of Chemistry, Central Mindanao University, Bukidnon, Philippines.
FAU - Weiland, Florian
AU  - Weiland F
AUID- ORCID: 0000-0001-9702-9245
AD  - Adelaide Proteomics Centre, School of Biological Sciences, University of 
      Adelaide, Adelaide, South Australia, 5005, Australia.
AD  - Laboratory of Enzyme, Fermentation and Brewing Technology (EFBT), Department of 
      Microbial and Molecular Systems, Technology Campus Ghent, KU Leuven, Leuven, 
      Belgium.
FAU - Mittal, Parul
AU  - Mittal P
AUID- ORCID: 0000-0003-0139-9757
AD  - Adelaide Proteomics Centre, School of Biological Sciences, University of 
      Adelaide, Adelaide, South Australia, 5005, Australia.
AD  - Future Industries Institute, University of South Australia, Adelaide, South 
      Australia, 5095, Australia.
FAU - Hoffmann, Peter
AU  - Hoffmann P
AUID- ORCID: 0000-0001-8493-2208
AD  - Adelaide Proteomics Centre, School of Biological Sciences, University of 
      Adelaide, Adelaide, South Australia, 5005, Australia.
AD  - Future Industries Institute, University of South Australia, Adelaide, South 
      Australia, 5095, Australia.
FAU - Sillero-Mahinay, Myrna
AU  - Sillero-Mahinay M
AD  - Department of Chemistry, MSU-Iligan Institute of Technology, Iligan City, 
      Philippines.
FAU - Pukala, Tara
AU  - Pukala T
AUID- ORCID: 0000-0001-7391-1436
AD  - Adelaide Proteomics Centre, School of Biological Sciences, University of 
      Adelaide, Adelaide, South Australia, 5005, Australia.
AD  - School of Physical Sciences, University of Adelaide, Adelaide, South Australia, 
      5005, Australia.
LA  - eng
GR  - Commission on Higher Education Scholarship Grant (Ph.D. Sandwich Program) of the 
      Philippine Government/
PT  - Journal Article
DEP - 20201120
PL  - England
TA  - J Mass Spectrom
JT  - Journal of mass spectrometry : JMS
JID - 9504818
SB  - IM
OTO - NOTNLM
OT  - differential protein abundance
OT  - leaf proteome
OT  - metal hyperaccumulators
OT  - phytoremediation
OT  - protein profiling
EDAT- 2020/11/29 06:00
MHDA- 2020/11/29 06:01
CRDT- 2020/11/28 05:37
PHST- 2020/11/12 00:00 [revised]
PHST- 2020/05/27 00:00 [received]
PHST- 2020/11/17 00:00 [accepted]
PHST- 2020/11/29 06:01 [medline]
PHST- 2020/11/29 06:00 [pubmed]
PHST- 2020/11/28 05:37 [entrez]
AID - 10.1002/jms.4689 [doi]
PST - aheadofprint
SO  - J Mass Spectrom. 2020 Nov 20;56(4):e4689. doi: 10.1002/jms.4689.