PMID- 33250889
OWN - NLM
STAT- MEDLINE
DCOM- 20210430
LR  - 20210430
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 11
DP  - 2020
TI  - Association Between Nitric Oxide, Oxidative Stress, Eryptosis, Red Blood Cell 
      Microparticles, and Vascular Function in Sickle Cell Anemia.
PG  - 551441
LID - 10.3389/fimmu.2020.551441 [doi]
LID - 551441
AB  - Chronic hemolysis, enhanced oxidative stress, and decreased nitric oxide (NO) 
      bioavailability promote vasculopathy in sickle cell anemia (SCA). Oxidative 
      stress and NO are known to modulate eryptosis in healthy red blood cells (RBCs); 
      however, their role in SCA eryptosis and their impact on the genesis of 
      RBC-derived microparticles (RBC-MPs) remains poorly described. RBC-MPs could play 
      a role in vascular dysfunction in SCA. The aims of this study were to evaluate 
      the roles of oxidative stress and NO in eryptosis and RBC-MPs release, and to 
      determine whether RBC-MPs could be involved in vascular dysfunction in SCA. 
      Markers of eryptosis and oxidative stress, plasma RBC-MPs concentration and 
      arterial stiffness were compared between SCA and healthy (AA) individuals. 
      In-vitro experiments were performed to test: 1) the effects of oxidative stress 
      (antioxidant: n-acetylcysteine (NAC); pro-oxidant: cumene hydroperoxide) and NO 
      (NO donor: sodium nitroprusside (SNP); NO-synthase inhibitor (L-NIO)) on 
      eryptosis, RBC deformability and RBC-MP genesis; 2) the effects of SCA/AA-RBC-MPs 
      on human aortic endothelial cell (HAEC) inflammatory phenotype and TLR4 pathway. 
      Eryptosis, RBC-MPs, oxidative stress and arterial stiffness were increased in 
      SCA. NAC increased RBC deformability and decreased eryptosis and RBC-MPs release, 
      while cumene did the opposite. SNP increased RBC deformability and limited 
      eryptosis, but had no effect on RBC-MPs. L-NIO did not affect these parameters. 
      Arterial stiffness was correlated with RBC-MPs concentration in SCA. RBC-MPs 
      isolated directly from SCA blood increased adhesion molecules expression and the 
      production of cytokines by HAEC compared to those isolated from AA blood. TLR4 
      inhibition alleviated these effects. Our data show that oxidative stress could 
      promote eryptosis and the release of RBC-MPs that are potentially involved in 
      macrovascular dysfunction in SCA.
CI  - Copyright (c) 2020 Nader, Romana, Guillot, Fort, Stauffer, Lemonne, Garnier, 
      Skinner, Etienne-Julan, Robert, Gauthier, Cannas, Antoine-Jonville, Tressieres, 
      Hardy-Dessources, Bertrand, Martin, Renoux, Joly, Grau and Connes.
FAU - Nader, Elie
AU  - Nader E
AD  - Laboratoire Interuniversitaire de Biologie de la Motricite (LIBM) EA7424, Team << 
      Vascular Biology and Red Blood Cell >>, Universite Claude Bernard Lyon 1, 
      Universite de Lyon, Lyon, France.
AD  - Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, 
      France.
FAU - Romana, Marc
AU  - Romana M
AD  - Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, 
      France.
AD  - Universite des Antilles, Pointe-a-Pitre, France.
AD  - Universite de Paris, Paris, France.
FAU - Guillot, Nicolas
AU  - Guillot N
AD  - Laboratoire Carmen Inserm, Universite Claude Bernard Lyon 1, Universite de Lyon, 
      Villeurbanne, France.
FAU - Fort, Romain
AU  - Fort R
AD  - Laboratoire Interuniversitaire de Biologie de la Motricite (LIBM) EA7424, Team << 
      Vascular Biology and Red Blood Cell >>, Universite Claude Bernard Lyon 1, 
      Universite de Lyon, Lyon, France.
AD  - Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, 
      France.
AD  - Departement de Medecine Interne, Hopital Edouard Herriot, Hospices Civils de 
      Lyon, Lyon, France.
FAU - Stauffer, Emeric
AU  - Stauffer E
AD  - Laboratoire Interuniversitaire de Biologie de la Motricite (LIBM) EA7424, Team << 
      Vascular Biology and Red Blood Cell >>, Universite Claude Bernard Lyon 1, 
      Universite de Lyon, Lyon, France.
AD  - Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, 
      France.
AD  - Centre de Medecine du Sommeil et des Maladies Respiratoires, Hospices Civils de 
      Lyon, Hopital de la Croix Rousse, Lyon, France.
FAU - Lemonne, Nathalie
AU  - Lemonne N
AD  - Unite Transversale de la Drepanocytose, Hopital de Pointe-a-Pitre, Hopital Ricou, 
      Guadeloupe, France.
FAU - Garnier, Yohann
AU  - Garnier Y
AD  - Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, 
      France.
AD  - Universite des Antilles, Pointe-a-Pitre, France.
AD  - Universite de Paris, Paris, France.
FAU - Skinner, Sarah Chambers
AU  - Skinner SC
AD  - Laboratoire Interuniversitaire de Biologie de la Motricite (LIBM) EA7424, Team << 
      Vascular Biology and Red Blood Cell >>, Universite Claude Bernard Lyon 1, 
      Universite de Lyon, Lyon, France.
AD  - Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, 
      France.
FAU - Etienne-Julan, Maryse
AU  - Etienne-Julan M
AD  - Unite Transversale de la Drepanocytose, Hopital de Pointe-a-Pitre, Hopital Ricou, 
      Guadeloupe, France.
FAU - Robert, Melanie
AU  - Robert M
AD  - Erytech Pharma, Lyon, France.
FAU - Gauthier, Alexandra
AU  - Gauthier A
AD  - Laboratoire Interuniversitaire de Biologie de la Motricite (LIBM) EA7424, Team << 
      Vascular Biology and Red Blood Cell >>, Universite Claude Bernard Lyon 1, 
      Universite de Lyon, Lyon, France.
AD  - Institut d'Hematologie et d'Oncologie Pediatrique, Hospices Civils de Lyon, Lyon, 
      France.
FAU - Cannas, Giovanna
AU  - Cannas G
AD  - Laboratoire Interuniversitaire de Biologie de la Motricite (LIBM) EA7424, Team << 
      Vascular Biology and Red Blood Cell >>, Universite Claude Bernard Lyon 1, 
      Universite de Lyon, Lyon, France.
AD  - Departement de Medecine Interne, Hopital Edouard Herriot, Hospices Civils de 
      Lyon, Lyon, France.
FAU - Antoine-Jonville, Sophie
AU  - Antoine-Jonville S
AD  - Universite des Antilles, Pointe-a-Pitre, France.
FAU - Tressieres, Benoit
AU  - Tressieres B
AD  - Centre Investigation Clinique Antilles Guyane, 1424 Inserm, Academic Hospital of 
      Pointe-a-Pitre, Pointe-a-Pitre, Guadeloupe, France.
FAU - Hardy-Dessources, Marie-Dominique
AU  - Hardy-Dessources MD
AD  - Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, 
      France.
AD  - Universite des Antilles, Pointe-a-Pitre, France.
AD  - Universite de Paris, Paris, France.
FAU - Bertrand, Yves
AU  - Bertrand Y
AD  - Institut d'Hematologie et d'Oncologie Pediatrique, Hospices Civils de Lyon, Lyon, 
      France.
FAU - Martin, Cyril
AU  - Martin C
AD  - Laboratoire Interuniversitaire de Biologie de la Motricite (LIBM) EA7424, Team << 
      Vascular Biology and Red Blood Cell >>, Universite Claude Bernard Lyon 1, 
      Universite de Lyon, Lyon, France.
AD  - Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, 
      France.
FAU - Renoux, Celine
AU  - Renoux C
AD  - Laboratoire Interuniversitaire de Biologie de la Motricite (LIBM) EA7424, Team << 
      Vascular Biology and Red Blood Cell >>, Universite Claude Bernard Lyon 1, 
      Universite de Lyon, Lyon, France.
AD  - Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, 
      France.
AD  - Laboratoire de Biochimie et de Biologie Moleculaire, UF de Biochimie des 
      Pathologies erythrocytaires, Centre de Biologie et de Pathologie Est, Hospices 
      Civils de Lyon, Lyon, France.
FAU - Joly, Philippe
AU  - Joly P
AD  - Laboratoire Interuniversitaire de Biologie de la Motricite (LIBM) EA7424, Team << 
      Vascular Biology and Red Blood Cell >>, Universite Claude Bernard Lyon 1, 
      Universite de Lyon, Lyon, France.
AD  - Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, 
      France.
AD  - Laboratoire de Biochimie et de Biologie Moleculaire, UF de Biochimie des 
      Pathologies erythrocytaires, Centre de Biologie et de Pathologie Est, Hospices 
      Civils de Lyon, Lyon, France.
FAU - Grau, Marijke
AU  - Grau M
AD  - Molecular and Cellular Sport Medicine, Deutsche Sporthochschule Koln, Koln, 
      Germany.
FAU - Connes, Philippe
AU  - Connes P
AD  - Laboratoire Interuniversitaire de Biologie de la Motricite (LIBM) EA7424, Team << 
      Vascular Biology and Red Blood Cell >>, Universite Claude Bernard Lyon 1, 
      Universite de Lyon, Lyon, France.
AD  - Laboratoire d'Excellence du Globule Rouge (Labex GR-Ex), PRES Sorbonne, Paris, 
      France.
AD  - Institut Universitaire de France, Paris, France.
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
DEP - 20201104
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Anemia, Sickle Cell/*blood/pathology
MH  - Cell-Derived Microparticles/*metabolism/pathology
MH  - Child
MH  - Child, Preschool
MH  - *Eryptosis
MH  - Erythrocytes, Abnormal/*metabolism/pathology
MH  - Female
MH  - Humans
MH  - Male
MH  - Nitric Oxide/*blood
MH  - *Oxidative Stress
MH  - *Vascular Stiffness
PMC - PMC7672038
OTO - NOTNLM
OT  - TLR4
OT  - endothelial cells
OT  - eryptosis
OT  - red blood cell microparticles
OT  - sickle cell anemia
OT  - vascular dysfunction
EDAT- 2020/12/01 06:00
MHDA- 2021/05/01 06:00
PMCR- 2020/01/01
CRDT- 2020/11/30 05:53
PHST- 2020/04/13 00:00 [received]
PHST- 2020/10/14 00:00 [accepted]
PHST- 2020/11/30 05:53 [entrez]
PHST- 2020/12/01 06:00 [pubmed]
PHST- 2021/05/01 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2020.551441 [doi]
PST - epublish
SO  - Front Immunol. 2020 Nov 4;11:551441. doi: 10.3389/fimmu.2020.551441. eCollection 
      2020.