PMID- 33251045
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201201
IS  - 2162-2531 (Print)
IS  - 2162-2531 (Electronic)
IS  - 2162-2531 (Linking)
VI  - 22
DP  - 2020 Dec 4
TI  - lncRNA SNHG10 Promotes the Proliferation and Invasion of Osteosarcoma via 
      Wnt/beta-Catenin Signaling.
PG  - 957-970
LID - 10.1016/j.omtn.2020.10.010 [doi]
AB  - Uncontrolled growth and an enforced epithelial-mesenchymal transition (EMT) 
      process contribute to the poor survival rate of patients with osteosarcoma (OS). 
      Long noncoding RNAs (lncRNAs) have been reported to be involved in the 
      development of OS. However, the significant role of lncRNA SNHG1O on regulating 
      proliferation and the EMT process of OS cells remains unclear. In this study, 
      quantitative real-time PCR and fluorescence in situ hybridization (FISH) results 
      suggested that SNHG10 levels were significantly increased in OS compared with 
      healthy tissues. In vitro experiments (including colony formation, CCK-8, wound 
      healing, and transwell assays) and in vivo experiments indicated that 
      downregulation of SNHG10 significantly suppressed the proliferation and invasion 
      of OS cells. Luciferase reporter assay and RNA immunoprecipitation (RIP) assay 
      confirmed that SNHG10 could regulate FZD3 levels through sponging microRNA 182-5p 
      (miR-182-5p). In addition, the SNHG10/miR-182-5p/FZD3 axis could further promote 
      the beta-catenin transfer into nuclear accumulation to maintain the activation of 
      the Wnt singling pathway. Together, our results established that SNHG10 has an 
      important role in promoting OS growth and invasion. By sponging miR-182-5p, 
      SNHG10 can increase FZD3 expression and further maintain the activation of 
      Wnt/beta-catenin singling pathway in OS cells.
CI  - (c) 2020 The Authors.
FAU - Zhu, Shutao
AU  - Zhu S
AD  - Department of Orthopedics, Huaihe Hospital of Henan University, Kaifeng City, 
      Henan, China.
FAU - Liu, Yang
AU  - Liu Y
AD  - Department of Orthopedics, Huaihe Hospital of Henan University, Kaifeng City, 
      Henan, China.
FAU - Wang, Xiao
AU  - Wang X
AD  - Department of Orthopedics, Huaihe Hospital of Henan University, Kaifeng City, 
      Henan, China.
FAU - Wang, Junyi
AU  - Wang J
AD  - Department of Orthopedics, Huaihe Hospital of Henan University, Kaifeng City, 
      Henan, China.
FAU - Xi, Guanghui
AU  - Xi G
AD  - Department of Orthopedics, Huaihe Hospital of Henan University, Kaifeng City, 
      Henan, China.
LA  - eng
PT  - Journal Article
DEP - 20201015
PL  - United States
TA  - Mol Ther Nucleic Acids
JT  - Molecular therapy. Nucleic acids
JID - 101581621
PMC - PMC7674123
OTO - NOTNLM
OT  - EMT
OT  - SNHG10
OT  - Wnt/beta-catenin
OT  - lncRNA
OT  - osteosarcoma
EDAT- 2020/12/01 06:00
MHDA- 2020/12/01 06:01
PMCR- 2020/10/15
CRDT- 2020/11/30 05:54
PHST- 2020/07/04 00:00 [received]
PHST- 2020/10/10 00:00 [accepted]
PHST- 2020/11/30 05:54 [entrez]
PHST- 2020/12/01 06:00 [pubmed]
PHST- 2020/12/01 06:01 [medline]
PHST- 2020/10/15 00:00 [pmc-release]
AID - S2162-2531(20)30322-X [pii]
AID - 10.1016/j.omtn.2020.10.010 [doi]
PST - epublish
SO  - Mol Ther Nucleic Acids. 2020 Oct 15;22:957-970. doi: 10.1016/j.omtn.2020.10.010. 
      eCollection 2020 Dec 4.