PMID- 33252329
OWN - NLM
STAT- MEDLINE
DCOM- 20210401
LR  - 20240330
IS  - 2050-084X (Electronic)
IS  - 2050-084X (Linking)
VI  - 9
DP  - 2020 Nov 30
TI  - Presynaptic NMDARs cooperate with local spikes toward GABA release from the 
      reciprocal olfactory bulb granule cell spine.
LID - 10.7554/eLife.63737 [doi]
LID - e63737
AB  - In the rodent olfactory bulb the smooth dendrites of the principal glutamatergic 
      mitral cells (MCs) form reciprocal dendrodendritic synapses with large spines on 
      GABAergic granule cells (GC), where unitary release of glutamate can trigger 
      postsynaptic local activation of voltage-gated Na(+)-channels (Na(v)s), that is a 
      spine spike. Can such single MC input evoke reciprocal release? We find that 
      unitary-like activation via two-photon uncaging of glutamate causes GC spines to 
      release GABA both synchronously and asynchronously onto MC dendrites. This 
      release indeed requires activation of Na(v)s and high-voltage-activated 
      Ca(2+)-channels (HVACCs), but also of NMDA receptors (NMDAR). Simulations show 
      temporally overlapping HVACC- and NMDAR-mediated Ca(2+)-currents during the spine 
      spike, and ultrastructural data prove NMDAR presence within the GABAergic 
      presynapse. This cooperative action of presynaptic NMDARs allows to implement 
      synapse-specific, activity-dependent lateral inhibition, and thus could provide 
      an efficient solution to combinatorial percept synthesis in a sensory system with 
      many receptor channels.
CI  - (c) 2020, Lage-Rupprecht et al.
FAU - Lage-Rupprecht, Vanessa
AU  - Lage-Rupprecht V
AD  - Neurophysiology, Institute of Zoology, Universitat Regensburg, Regensburg, 
      Germany.
AD  - Department of Bioinformatics, Fraunhofer SCAI, Sankt Augustin, Germany.
FAU - Zhou, Li
AU  - Zhou L
AD  - Neurophysiology, Institute of Zoology, Universitat Regensburg, Regensburg, 
      Germany.
FAU - Bianchini, Gaia
AU  - Bianchini G
AD  - Neurophysiology, Institute of Zoology, Universitat Regensburg, Regensburg, 
      Germany.
FAU - Aghvami, S Sara
AU  - Aghvami SS
AD  - Neurophysiology, Institute of Zoology, Universitat Regensburg, Regensburg, 
      Germany.
AD  - School of Electrical and Computer Engineering, University of Tehran, Tehran, 
      Islamic Republic of Iran.
AD  - School of Cognitive Sciences, Institute for Research in Fundamental Sciences 
      (IPM), Tehran, Islamic Republic of Iran.
FAU - Mueller, Max
AU  - Mueller M
AD  - Neurophysiology, Institute of Zoology, Universitat Regensburg, Regensburg, 
      Germany.
FAU - Rozsa, Balazs
AU  - Rozsa B
AD  - Two-Photon Imaging Center, Institute of Experimental Medicine, Hungarian Academy 
      of Sciences, Budapest, Hungary.
FAU - Sassoe-Pognetto, Marco
AU  - Sassoe-Pognetto M
AD  - Neuroscience Department, University of Turin, Torino, Italy.
FAU - Egger, Veronica
AU  - Egger V
AUID- ORCID: 0000-0002-5869-8523
AD  - Neurophysiology, Institute of Zoology, Universitat Regensburg, Regensburg, 
      Germany.
LA  - eng
SI  - Dryad/10.5061/dryad.t4b8gtj04
GR  - 01GQ1104/01GQ1502/Bundesministerium fur Bildung und Forschung/International
GR  - 1479-418.13/German-Israeli Foundation for Scientific Research and 
      Development/International
GR  - DFG SFB 870/Graduate School of Systemic Neurosciences GSN-LMU/International
GR  - 682426/ERC_/European Research Council/International
GR  - GINOP_2.1.1-15-2016-00979/ERC_/European Research Council/International
GR  - VKSZ_14-1-2015-0155/ERC_/European Research Council/International
GR  - 712821-NEURAM/ERC_/European Research Council/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201130
PL  - England
TA  - Elife
JT  - eLife
JID - 101579614
RN  - 0 (Calcium Channels)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (Sodium Channels)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
SB  - IM
MH  - Action Potentials/physiology
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Calcium Channels
MH  - Dendritic Cells/*physiology
MH  - Electric Stimulation
MH  - Female
MH  - Gene Expression Regulation
MH  - Ion Channel Gating
MH  - Male
MH  - Neurons/*physiology
MH  - Olfactory Bulb/*cytology
MH  - Patch-Clamp Techniques
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, N-Methyl-D-Aspartate/genetics/*metabolism
MH  - Sodium Channels
MH  - gamma-Aminobutyric Acid/genetics/*metabolism
PMC - PMC7704106
OTO - NOTNLM
OT  - granule cell
OT  - mitral cell
OT  - neuroscience
OT  - olfactory bulb
OT  - presynaptic NMDA receptor
OT  - rat
OT  - reciprocal synapse
COIS- VL, LZ, GB, SA, MM, MS, VE No competing interests declared, BR BR is a founder of 
      Femtonics Kft and a member of its scientific advisory board. No other competing 
      interests exist.
EDAT- 2020/12/01 06:00
MHDA- 2021/04/02 06:00
PMCR- 2020/11/30
CRDT- 2020/11/30 12:09
PHST- 2020/10/05 00:00 [received]
PHST- 2020/11/10 00:00 [accepted]
PHST- 2020/11/30 12:09 [entrez]
PHST- 2020/12/01 06:00 [pubmed]
PHST- 2021/04/02 06:00 [medline]
PHST- 2020/11/30 00:00 [pmc-release]
AID - 63737 [pii]
AID - 10.7554/eLife.63737 [doi]
PST - epublish
SO  - Elife. 2020 Nov 30;9:e63737. doi: 10.7554/eLife.63737.