PMID- 33254999
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 118
DP  - 2021 Jan
TI  - A new Ag-nanostructured hydroxyapatite porous scaffold: Antibacterial effect and 
      cytotoxicity study.
PG  - 111394
LID - S0928-4931(20)33312-9 [pii]
LID - 10.1016/j.msec.2020.111394 [doi]
AB  - We report a new chemical method for the functionalization of Mg-hydroxyapatite 
      (Mg-HA) scaffold with Ag nanoparticles (Ag NPs) integrating in one step both the 
      synthesis of the Ag NPs and their nano-structuring into the HA matrix (Ag-Mg-HA 
      scaffold). This method exploits a green photochemical synthesis and allows the 
      direct growth of Ag NPs on the Mg-HA surface. The surface structure of Ag-Mg-HA 
      scaffold, investigated by scanning electron microscopy, shows no significant 
      changes in the morphology upon Ag NPs incorporation. The presence of Ag was 
      confirmed by EDX analysis. TEM and spectroscopic investigations show Ag NPs 
      spherical shaped with a mean diameter of about 20 nm exhibiting the typical 
      plasmon absorption band with maximum at 420 nm. The antibacterial properties of 
      Ag-Mg-HA scaffolds were tested against two bacterial strains, Escherichia coli 
      (E. coli) and Staphylococcus aureus (S. aureus). The results show excellent 
      antibacterial properties achieving up to 99% and 100% reduction of colonies for 
      both bacteria cultures after 24 h of incubation and 100% of reduction after 48 h 
      of incubation. The cytotoxicity of Ag-Mg-HA was also in deep investigated 
      assessing both cell proliferation and differentiation using hADSCs (human Adipose 
      Derived Stem Cells) and testing data point at 0, 7, 14 and 24 days. The results 
      show cytotoxic effect with cell proliferation decreasing up to 90% at 24 days and 
      osteogenic differentiation inhibition. The observed cytotoxicity can be probable 
      ascribed to the oxidative stress by ROS. Indeed, considering the effectiveness of 
      the nanofunctionalization method and the excellent antibacterial properties 
      showed by the Ag-Mg-HA scaffold, future works will be devoted to create 
      nanofunctionalized scaffold satisfying both antimicrobial and osteo-regenerative 
      properties.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Calabrese, Giovanna
AU  - Calabrese G
AD  - ChiBioFarAm Department, University of Messina, Messina, Italy.
FAU - Petralia, Salvatore
AU  - Petralia S
AD  - Department of Drug Science Department, University of Catania, Catania, Italy.
FAU - Franco, Domenico
AU  - Franco D
AD  - ChiBioFarAm Department, University of Messina, Messina, Italy.
FAU - Nocito, Giuseppe
AU  - Nocito G
AD  - ChiBioFarAm Department, University of Messina, Messina, Italy.
FAU - Fabbi, Claudia
AU  - Fabbi C
AD  - Fin-Ceramica Faenza, 48018 Faenza, RA, Italy.
FAU - Forte, Lucia
AU  - Forte L
AD  - Fin-Ceramica Faenza, 48018 Faenza, RA, Italy.
FAU - Guglielmino, Salvatore
AU  - Guglielmino S
AD  - ChiBioFarAm Department, University of Messina, Messina, Italy.
FAU - Squarzoni, Stefano
AU  - Squarzoni S
AD  - CNR - Institute of Molecular Genetics "Luigi Luca Cavalli-Sforza", Bologna, 
      Italy; IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy.
FAU - Traina, Francesco
AU  - Traina F
AD  - IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy; Biomorf Department, University 
      of Messina, Messina, Italy.
FAU - Conoci, Sabrina
AU  - Conoci S
AD  - ChiBioFarAm Department, University of Messina, Messina, Italy; Distretto 
      Tecnologico Micro e Nano Sistemi Sicilia, Catania, Italy. Electronic address: 
      sabrina.conoci@unime.it.
LA  - eng
PT  - Journal Article
DEP - 20200822
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 0 (Anti-Bacterial Agents)
RN  - 3M4G523W1G (Silver)
RN  - 91D9GV0Z28 (Durapatite)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - Durapatite
MH  - Escherichia coli
MH  - Humans
MH  - *Metal Nanoparticles
MH  - Osteogenesis
MH  - Porosity
MH  - *Silver
MH  - Staphylococcus aureus
OTO - NOTNLM
OT  - Ag
OT  - Antibacterial
OT  - Citotoxicity
OT  - Hydroxyapatite
OT  - Nanoparticles
EDAT- 2020/12/02 06:00
MHDA- 2021/05/15 06:00
CRDT- 2020/12/01 01:05
PHST- 2020/06/21 00:00 [received]
PHST- 2020/08/07 00:00 [revised]
PHST- 2020/08/19 00:00 [accepted]
PHST- 2020/12/01 01:05 [entrez]
PHST- 2020/12/02 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
AID - S0928-4931(20)33312-9 [pii]
AID - 10.1016/j.msec.2020.111394 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2021 Jan;118:111394. doi: 
      10.1016/j.msec.2020.111394. Epub 2020 Aug 22.