PMID- 33255065
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 118
DP  - 2021 Jan
TI  - Synthesis, characterization and in vitro cytotoxicity studies of poly-N-isopropyl 
      acrylamide gel nanoparticles and films.
PG  - 111507
LID - S0928-4931(20)33425-1 [pii]
LID - 10.1016/j.msec.2020.111507 [doi]
AB  - In this work, we show synthesis that leads to thermoreponsive poly-N-isopropyl 
      acrylamide (pNIPAM) nanogels with sizes below 100 nm, irrespectively of the 
      surfactant to crosslinker ratio. We also show that in many environments the 
      temperature induced pNIPAM collapse at Lower Critical Solution Temperature (LCST) 
      of 32.5  degrees C is accompanied by gel nanoparticles' aggregation. Thus, the proper 
      information on the nanoparticle (NP) structure and deswelling can be obtained 
      only if the routinely measured hydrodynamic radius is supplemented by information 
      on the molecular weight, which can be obtained from the intensity of scattered 
      light. We measured the dynamics and reversibility of the deswelling and 
      subsequent aggregation processes. Furthermore, we show that the highly 
      concentrated pNIPAM gel NPs reversibly form bulk hydrogel networks of varied 
      interconnected porous structure. We show, that in case of drying pNIPAM gel NPs 
      above the LCST, it is possible to obtain films with 20-fold increase in storage 
      modulus (G') compared to hydrogel networks measured at room temperature. They 
      exhibit temperature hysteresis behavior around LCST of 32.5  degrees C similar to pNIPAM 
      films. Finally, we show that these hydrogel films, lead to extended proliferation 
      of cells across three different types: fibroblast, endothelial and cancer cells. 
      Additionally, none of the films exhibited any cytotoxic effects. Our study brings 
      new insights into physicochemical characterization of pNIPAM gel NPs and networks 
      behavior in realistic conditions of in vitro measurements, especially by means of 
      dynamic light scattering as well as final unique properties of both gel NPs and 
      formed porous films for possible tissue engineering applications.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Litowczenko, Jagoda
AU  - Litowczenko J
AD  - NanoBioMedical Centre, Adam Mickiewicz University in Poznan, ul. Wszechnicy 
      Piastowskiej 3, 61-614 Poznan, Poland.
FAU - Gapinski, Jacek
AU  - Gapinski J
AD  - Department of Molecular Biophysics, Faculty of Physics, Adam Mickiewicz 
      University in Poznan, ul. Uniwersytetu Poznanskiego 3, 61-614 Poznan, Poland.
FAU - Markiewicz, Roksana
AU  - Markiewicz R
AD  - NanoBioMedical Centre, Adam Mickiewicz University in Poznan, ul. Wszechnicy 
      Piastowskiej 3, 61-614 Poznan, Poland.
FAU - Wozniak, Anna
AU  - Wozniak A
AD  - NanoBioMedical Centre, Adam Mickiewicz University in Poznan, ul. Wszechnicy 
      Piastowskiej 3, 61-614 Poznan, Poland.
FAU - Wychowaniec, Jacek K
AU  - Wychowaniec JK
AD  - NanoBioMedical Centre, Adam Mickiewicz University in Poznan, ul. Wszechnicy 
      Piastowskiej 3, 61-614 Poznan, Poland.
FAU - Peplinska, Barbara
AU  - Peplinska B
AD  - NanoBioMedical Centre, Adam Mickiewicz University in Poznan, ul. Wszechnicy 
      Piastowskiej 3, 61-614 Poznan, Poland.
FAU - Jurga, Stefan
AU  - Jurga S
AD  - NanoBioMedical Centre, Adam Mickiewicz University in Poznan, ul. Wszechnicy 
      Piastowskiej 3, 61-614 Poznan, Poland.
FAU - Patkowski, Adam
AU  - Patkowski A
AD  - NanoBioMedical Centre, Adam Mickiewicz University in Poznan, ul. Wszechnicy 
      Piastowskiej 3, 61-614 Poznan, Poland; Department of Molecular Biophysics, 
      Faculty of Physics, Adam Mickiewicz University in Poznan, ul. Uniwersytetu 
      Poznanskiego 3, 61-614 Poznan, Poland. Electronic address: 
      adam.patkowski@amu.edu.pl.
LA  - eng
PT  - Journal Article
DEP - 20200911
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 0 (Acrylic Resins)
RN  - 0 (Hydrogels)
RN  - 25189-55-3 (poly-N-isopropylacrylamide)
SB  - IM
MH  - *Acrylic Resins
MH  - Hydrogels
MH  - *Nanoparticles/toxicity
MH  - Temperature
OTO - NOTNLM
OT  - Cell cytotoxicity
OT  - Dynamic light scattering
OT  - Films
OT  - Hydrogels
OT  - Poly-N-isopropyl acrylamide (pNIPAM)
OT  - Swelling and deswelling properties
EDAT- 2020/12/02 06:00
MHDA- 2021/05/15 06:00
CRDT- 2020/12/01 01:05
PHST- 2020/04/30 00:00 [received]
PHST- 2020/07/02 00:00 [revised]
PHST- 2020/09/07 00:00 [accepted]
PHST- 2020/12/01 01:05 [entrez]
PHST- 2020/12/02 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
AID - S0928-4931(20)33425-1 [pii]
AID - 10.1016/j.msec.2020.111507 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2021 Jan;118:111507. doi: 
      10.1016/j.msec.2020.111507. Epub 2020 Sep 11.