PMID- 33255151
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201226
IS  - 2072-666X (Print)
IS  - 2072-666X (Electronic)
IS  - 2072-666X (Linking)
VI  - 11
IP  - 12
DP  - 2020 Nov 24
TI  - A Double-Deck Self-Digitization Microfluidic Chip for Digital PCR.
LID - 10.3390/mi11121025 [doi]
LID - 1025
AB  - In this work, a double-deck microfluidic chip was presented for digital PCR 
      application. This chip consists of two reverse-placed micro-patterned 
      polydimethylsiloxane (PDMS) layers between the top and bottom glass substrates. 
      Each micropatterned PDMS layer contains more than 20,000 cylindrical 
      micro-chambers to hold the partitioned droplets. The double-deck designs can 
      double the number of chambers and reagent capacity without changing the planar 
      area of the chip. In addition, carbon black was mixed into the pure PDMS gel to 
      obstruct the passage of fluorescence from the positive chambers between the two 
      layers of the chip. Thus, the fluorescence signal of micro-chambers in different 
      layers of the chip after PCR can be collected without mutual interference. The 
      quantitative capability of the proposed chip was evaluated by measuring a 10-fold 
      serial dilution of the DNA template. A high accuracy of the absolute 
      quantification for nucleic acid with a dynamic range of 10(5) was demonstrated by 
      this chip in this work. Owing to its characteristics of small planar area, large 
      capacity, and sensitivity, the double-deck microfluidic chip is expected to 
      further promote the extensive applications of digital PCR.
FAU - Xu, Gangwei
AU  - Xu G
AD  - State Key Laboratory of ASIC and System, School of Microelectronics, Fudan 
      University, Shanghai 200433, China.
FAU - Si, Huaqing
AU  - Si H
AD  - State Key Laboratory of ASIC and System, School of Microelectronics, Fudan 
      University, Shanghai 200433, China.
FAU - Jing, Fengxiang
AU  - Jing F
AD  - Turtle Technology Company Limited, Shanghai 200439, China.
FAU - Sun, Peng
AU  - Sun P
AD  - State Key Laboratory of ASIC and System, School of Microelectronics, Fudan 
      University, Shanghai 200433, China.
FAU - Zhao, Dan
AU  - Zhao D
AD  - State Key Laboratory of ASIC and System, School of Microelectronics, Fudan 
      University, Shanghai 200433, China.
FAU - Wu, Dongping
AU  - Wu D
AD  - State Key Laboratory of ASIC and System, School of Microelectronics, Fudan 
      University, Shanghai 200433, China.
LA  - eng
GR  - 61474028/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20201124
PL  - Switzerland
TA  - Micromachines (Basel)
JT  - Micromachines
JID - 101640903
PMC - PMC7759810
OTO - NOTNLM
OT  - digital polymerase chain reaction
OT  - double-deck
OT  - microfluidic chip
COIS- The authors declare no conflict of interest.
EDAT- 2020/12/02 06:00
MHDA- 2020/12/02 06:01
PMCR- 2020/11/24
CRDT- 2020/12/01 01:07
PHST- 2020/11/04 00:00 [received]
PHST- 2020/11/18 00:00 [revised]
PHST- 2020/11/18 00:00 [accepted]
PHST- 2020/12/01 01:07 [entrez]
PHST- 2020/12/02 06:00 [pubmed]
PHST- 2020/12/02 06:01 [medline]
PHST- 2020/11/24 00:00 [pmc-release]
AID - mi11121025 [pii]
AID - micromachines-11-01025 [pii]
AID - 10.3390/mi11121025 [doi]
PST - epublish
SO  - Micromachines (Basel). 2020 Nov 24;11(12):1025. doi: 10.3390/mi11121025.