PMID- 3325523
OWN - NLM
STAT- MEDLINE
DCOM- 19880407
LR  - 20190510
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 2
IP  - 8
DP  - 1987 Nov
TI  - HLA and TLX antigen expression on the human oocyte, zona pellucida and granulosa 
      cells.
PG  - 657-64
AB  - The expression of human leukocyte antigen (HLA) class I and class II molecules 
      and one trophoblast-lymphocyte crossreactive (TLX) antigen by human oocytes and 
      granulosa cells was investigated. Well-defined monoclonal antibodies directed 
      against HLA class I and class II molecules as well as beta 2-microglobulin and a 
      TLX molecule were used in a standard indirect immunofluorescence test and some 
      immunogold techniques at the electron microscopic level. Single unfertilizable or 
      multiply fertilized oocytes and granulosa cells obtained from an in-vitro 
      fertilization programme as well as oocytes in primary, secondary and tertiary 
      follicles of ovaries were studied. Neither HLA class I or class II molecules, nor 
      beta 2-microglobulin, nor a TLX molecule were detected on cultured oocytes or 
      oocytes in human ovarian follicles or in their zona pellucida. Granulosa cells 
      taken from a culture medium and those in follicles at various stages of 
      development expressed class I antigens, while granulosa cells from tertiary 
      follicles also expressed HLA-DR antigens. These results confirm that the female 
      human gamete belongs to that very small group of cells that lack major 
      histocompatibility complex antigens. Since spermatozoa also lack HLA antigens, 
      human germ cells are entirely different from other nucleated human cells with 
      regard to the antigenic structures expressed on the cell surface. This would 
      prevent recognition of these cells by the cellular immune system. Furthermore, 
      HLA and TLX antigens are not involved in fertilization and early differentiation.
FAU - Dohr, G
AU  - Dohr G
AD  - Department of Histology and Embryology, KF University of Graz, Austria.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Autoantigens)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-C Antigens)
RN  - 0 (HLA-D Antigens)
RN  - 0 (HLA-DP Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Isoantigens)
RN  - 0 (trophoblast-lymphocyte cross-reactive antigens)
SB  - IM
MH  - Antibodies, Monoclonal/immunology
MH  - Autoantigens/*immunology
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Granulosa Cells/*immunology/ultrastructure
MH  - HLA Antigens/*immunology
MH  - HLA-A Antigens
MH  - HLA-B Antigens
MH  - HLA-C Antigens
MH  - HLA-D Antigens/immunology
MH  - HLA-DP Antigens/immunology
MH  - HLA-DR Antigens/immunology
MH  - Humans
MH  - In Vitro Techniques
MH  - *Isoantigens
MH  - Oocytes/*immunology/ultrastructure
MH  - Ovum/*immunology
MH  - Zona Pellucida/*immunology/ultrastructure
EDAT- 1987/11/01 00:00
MHDA- 1987/11/01 00:01
CRDT- 1987/11/01 00:00
PHST- 1987/11/01 00:00 [pubmed]
PHST- 1987/11/01 00:01 [medline]
PHST- 1987/11/01 00:00 [entrez]
AID - 10.1093/oxfordjournals.humrep.a136611 [doi]
PST - ppublish
SO  - Hum Reprod. 1987 Nov;2(8):657-64. doi: 10.1093/oxfordjournals.humrep.a136611.