PMID- 33255520
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201226
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 8
IP  - 12
DP  - 2020 Nov 25
TI  - Antioxidant/Anti-Inflammatory Effects of Caloric Restriction in an Aged and Obese 
      Rat Model: The Role of Adiponectin.
LID - 10.3390/biomedicines8120532 [doi]
LID - 532
AB  - Caloric restriction (CR) represents a powerful intervention for extending 
      healthspan and lifespan in several animal models, from yeast to primates. 
      Additionally, in humans, CR has been found to induce cardiometabolic adaptations 
      associated with improved health. In this study, we evaluated in an aged and obese 
      rat model the effect of long-term (6 months) caloric restriction (-40%) on the 
      oxidative/inflammatory balance in order to investigate the underlining 
      mechanisms. In plasma, we analyzed the oxidative balance by photometric tests and 
      the adiponectin/tumor necrosis factor-alpha-induced gene/protein 6 (TSG-6) levels by 
      Western blot analysis. In the white adipose tissue, we examined the protein 
      levels of AdipoR1, pAMPK, NFkappaB, NRF-2, and glutathione S-tranferase P1 by Western 
      blot analysis. Our results clearly showed that caloric restriction significantly 
      improves the plasmatic oxidative/inflammatory balance in parallel with a major 
      increase in circulating adiponectin levels. Additionally, at the level of adipose 
      tissue, we found a positive modulation of both anti-inflammatory and antioxidant 
      pathways. These adaptations, induced by caloric restriction, with the achievement 
      of normal weight, suggest that inflammatory and redox imbalance in obese aged 
      rats appear to be more linked to obesity than to aging.
FAU - La Russa, Daniele
AU  - La Russa D
AUID- ORCID: 0000-0003-1887-2854
AD  - Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 
      87036 Rende, Italy.
AD  - LARSO (Analysis and Research on Oxidative Stress Laboratory), University of 
      Calabria, 87036 Rende, Italy.
FAU - Marrone, Alessandro
AU  - Marrone A
AD  - LARSO (Analysis and Research on Oxidative Stress Laboratory), University of 
      Calabria, 87036 Rende, Italy.
AD  - Department of Biology, Ecology and Earth Sciences, University of Calabria, 87036 
      Rende, Italy.
FAU - Mandala, Maurizio
AU  - Mandala M
AUID- ORCID: 0000-0003-3736-0205
AD  - Department of Biology, Ecology and Earth Sciences, University of Calabria, 87036 
      Rende, Italy.
FAU - Macirella, Rachele
AU  - Macirella R
AUID- ORCID: 0000-0003-4121-9837
AD  - Department of Biology, Ecology and Earth Sciences, University of Calabria, 87036 
      Rende, Italy.
FAU - Pellegrino, Daniela
AU  - Pellegrino D
AUID- ORCID: 0000-0002-9815-5195
AD  - LARSO (Analysis and Research on Oxidative Stress Laboratory), University of 
      Calabria, 87036 Rende, Italy.
AD  - Department of Biology, Ecology and Earth Sciences, University of Calabria, 87036 
      Rende, Italy.
LA  - eng
PT  - Journal Article
DEP - 20201125
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC7761007
OTO - NOTNLM
OT  - adiponectin
OT  - caloric restriction
OT  - inflammation
OT  - oxidative balance
OT  - plasma
OT  - white adipose tissue
COIS- The authors declare no conflict of interest.
EDAT- 2020/12/02 06:00
MHDA- 2020/12/02 06:01
PMCR- 2020/11/25
CRDT- 2020/12/01 01:09
PHST- 2020/10/31 00:00 [received]
PHST- 2020/11/21 00:00 [revised]
PHST- 2020/11/23 00:00 [accepted]
PHST- 2020/12/01 01:09 [entrez]
PHST- 2020/12/02 06:00 [pubmed]
PHST- 2020/12/02 06:01 [medline]
PHST- 2020/11/25 00:00 [pmc-release]
AID - biomedicines8120532 [pii]
AID - biomedicines-08-00532 [pii]
AID - 10.3390/biomedicines8120532 [doi]
PST - epublish
SO  - Biomedicines. 2020 Nov 25;8(12):532. doi: 10.3390/biomedicines8120532.