PMID- 33256856 OWN - NLM STAT- MEDLINE DCOM- 20211215 LR - 20211215 IS - 1475-2662 (Electronic) IS - 0007-1145 (Linking) VI - 126 IP - 6 DP - 2021 Sep 28 TI - Nano chitosan-zinc complex improves the growth performance and antioxidant capacity of the small intestine in weaned piglets. PG - 801-812 LID - 10.1017/S0007114520004766 [doi] AB - The present study was conducted to test the hypothesis that dietary supplementation with a nano chitosan-zinc complex (CP-Zn, 100 mg/kg Zn) could alleviate weaning stress in piglets challenged with enterotoxigenic Escherichia coli K88 by improving growth performance and intestinal antioxidant capacity. The in vivo effects of CP-Zn on growth performance variables (including gastrointestinal digestion and absorption functions and the levels of key proteins related to muscle growth) and the antioxidant capacity of the small intestine (SI) were evaluated in seventy-two weaned piglets. The porcine jejunal epithelial cell line IPEC-J2 was used to further investigate the antioxidant mechanism of CP-Zn in vitro. The results showed that CP-Zn supplementation increased the jejunal villus height and decreased the diarrhoea rate in weaned piglets. CP-Zn supplementation also improved growth performance (average daily gain and average daily feed intake), increased the activity of carbohydrate digestion-related enzymes (amylase, maltase, sucrase and lactase) and the mRNA expression levels of nutrient transporters (Na+-dependent glucose transporter 1, glucose transporter type 2, peptide transporter 1 and excitatory amino acid carrier 1) in the jejunum and up-regulated the expression levels of mammalian target of rapamycin (mTOR) pathway-related proteins (insulin receptor substrate 1, phospho-mTOR and phospho-p70S6K) in muscle. In addition, CP-Zn supplementation increased glutathione content, enhanced total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-px) activity, and reduced malondialdehyde (MDA) content in the jejunum. Furthermore, CP-Zn decreased the content of MDA and reactive oxygen species, enhanced the activity of T-SOD and GSH-px and up-regulated the expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) pathway-related proteins (Nrf2, NAD(P)H:quinone oxidoreductase 1 and haeme oxygenase 1) in lipopolysaccharide-stimulated IPEC-J2 cells. Collectively, these findings indicate that CP-Zn supplementation can improve growth performance and the antioxidant capacity of the SI in piglets, thus alleviating weaning stress. FAU - Zhang, Minyang AU - Zhang M AD - Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing210095, People's Republic of China. AD - National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing210095, People's Republic of China. FAU - Hou, Guojun AU - Hou G AD - Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing210095, People's Republic of China. AD - National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing210095, People's Republic of China. FAU - Hu, Ping AU - Hu P AD - Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing210095, People's Republic of China. AD - National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing210095, People's Republic of China. FAU - Feng, Dan AU - Feng D AD - Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing210095, People's Republic of China. AD - National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing210095, People's Republic of China. FAU - Wang, Jing AU - Wang J AD - Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing210095, People's Republic of China. AD - National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing210095, People's Republic of China. AD - National Experimental Teaching Demonstration Center of Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing210095, People's Republic of China. FAU - Zhu, Weiyun AU - Zhu W AD - Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing210095, People's Republic of China. AD - National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing210095, People's Republic of China. AD - National Experimental Teaching Demonstration Center of Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing210095, People's Republic of China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20201201 PL - England TA - Br J Nutr JT - The British journal of nutrition JID - 0372547 RN - 0 (Antioxidants) RN - 0 (NF-E2-Related Factor 2) RN - 9012-76-4 (Chitosan) RN - EC 1.15.1.1 (Superoxide Dismutase) RN - EC 2.7.11.1 (TOR Serine-Threonine Kinases) RN - J41CSQ7QDS (Zinc) SB - IM MH - Animal Nutritional Physiological Phenomena MH - Animals MH - *Antioxidants/metabolism MH - *Chitosan/pharmacology MH - Diet/veterinary MH - *Dietary Supplements MH - Intestine, Small/*metabolism MH - NF-E2-Related Factor 2 MH - Superoxide Dismutase MH - Swine/*growth & development MH - TOR Serine-Threonine Kinases MH - Weaning MH - *Zinc OTO - NOTNLM OT - Antioxidant capacity OT - Chitosan-zinc nanoparticles OT - Growth performance OT - Small intestine OT - Weaned piglets EDAT- 2020/12/02 06:00 MHDA- 2021/12/16 06:00 CRDT- 2020/12/01 05:37 PHST- 2020/12/02 06:00 [pubmed] PHST- 2021/12/16 06:00 [medline] PHST- 2020/12/01 05:37 [entrez] AID - S0007114520004766 [pii] AID - 10.1017/S0007114520004766 [doi] PST - ppublish SO - Br J Nutr. 2021 Sep 28;126(6):801-812. doi: 10.1017/S0007114520004766. Epub 2020 Dec 1.