PMID- 33258323
OWN - NLM
STAT- MEDLINE
DCOM- 20220126
LR  - 20231106
IS  - 2466-054X (Electronic)
IS  - 2466-0493 (Print)
IS  - 2466-0493 (Linking)
VI  - 62
IP  - 1
DP  - 2021 Jan
TI  - Gene expression profiling of mouse cavernous endothelial cells for diagnostic 
      targets in diabetes-induced erectile dysfunction.
PG  - 90-99
LID - 10.4111/icu.20200119 [doi]
AB  - PURPOSE: To investigate potential target genes associated with the diabetic 
      condition in mouse cavernous endothelial cells (MCECs) for the treatment of 
      diabetes-induced erectile dysfunction (ED). MATERIALS AND METHODS: Mouse 
      cavernous tissue was embedded into Matrigel, and sprouted cells were 
      subcultivated for other studies. To mimic diabetic conditions, MCECs were exposed 
      to normal-glucose (NG, 5 mmoL) or high-glucose (HG, 30 mmoL) conditions for 72 
      hours. An RNA-sequencing assay was performed to evaluate gene expression 
      profiling, and RT-PCR was used to validate the sequencing data. RESULTS: We 
      isolated MCECs exposed to the two glucose conditions. MCECs showed well-organized 
      tubes and dynamic migration in the NG condition, whereas tube formation and 
      migration were significantly decreased in the HG condition. RNA-sequencing 
      analysis showed that MCECs had different gene profiles in the NG and HG 
      conditions. Among the significantly changed genes, which we classified into 14 
      major gene categories, we identified that aging-related (9.22%) and 
      angiogenesis-related (9.06%) genes were changed the most. Thirteen genes from the 
      two gene categories showed consistent changes on the RNA-sequencing assay, and 
      these findings were validated by RT-PCR. CONCLUSIONS: Our gene expression 
      profiling studies showed that Cyp1a1, Gclm, Igfbp5, Nqo1, Il6, Cxcl5, Olr1, Ctgf, 
      Hbegf, Serpine1, Cyr61, Angptl4, and Loxl2 may play a critical role in 
      diabetes-induced ED through aging and angiogenesis signaling. Additional research 
      is necessary to help us understand the potential mechanisms by which these genes 
      influence diabetes-induced ED.
CI  - (c) The Korean Urological Association, 2021.
FAU - Yin, Guo Nan
AU  - Yin GN
AUID- ORCID: 0000-0002-2512-7337
AD  - Department of Urology, National Research Center for Sexual Medicine, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Ock, Jiyeon
AU  - Ock J
AUID- ORCID: 0000-0002-8264-4939
AD  - Department of Urology, National Research Center for Sexual Medicine, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Choi, Min Ji
AU  - Choi MJ
AUID- ORCID: 0000-0002-5476-356X
AD  - Department of Urology, National Research Center for Sexual Medicine, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Limanjaya, Anita
AU  - Limanjaya A
AUID- ORCID: 0000-0003-3545-7660
AD  - Department of Urology, National Research Center for Sexual Medicine, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Ghatak, Kalyan
AU  - Ghatak K
AUID- ORCID: 0000-0002-1589-7362
AD  - Department of Urology, National Research Center for Sexual Medicine, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Song, Kang Moon
AU  - Song KM
AUID- ORCID: 0000-0001-8900-3301
AD  - Department of Urology, National Research Center for Sexual Medicine, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Kwon, Mi Hye
AU  - Kwon MH
AUID- ORCID: 0000-0001-7100-5819
AD  - Department of Urology, National Research Center for Sexual Medicine, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Suh, Jun Kyu
AU  - Suh JK
AUID- ORCID: 0000-0002-1812-9449
AD  - Department of Urology, National Research Center for Sexual Medicine, Inha 
      University School of Medicine, Incheon, Korea.
FAU - Ryu, Ji Kan
AU  - Ryu JK
AUID- ORCID: 0000-0003-0025-6025
AD  - Department of Urology, National Research Center for Sexual Medicine, Inha 
      University School of Medicine, Incheon, Korea. rjk0929@inha.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201109
PL  - Korea (South)
TA  - Investig Clin Urol
JT  - Investigative and clinical urology
JID - 101674989
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
CIN - Investig Clin Urol. 2023 Nov;64(6):606-607. PMID: 37932572
MH  - Aging/*genetics
MH  - Animals
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Diabetes Complications/*complications
MH  - Endothelial Cells/drug effects/pathology/*physiology
MH  - Erectile Dysfunction/etiology/*genetics
MH  - Gene Expression/*drug effects
MH  - Gene Expression Profiling
MH  - Gene Ontology
MH  - Glucose/pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neovascularization, Physiologic/genetics
MH  - Penis/blood supply
MH  - Primary Cell Culture
MH  - Sequence Analysis, RNA
PMC - PMC7801162
OTO - NOTNLM
OT  - Diabetes mellitus
OT  - Erectile dysfunction
OT  - Gene expression
OT  - Penis
OT  - RNA sequencing
COIS- The authors have nothing to disclose.
EDAT- 2020/12/02 06:00
MHDA- 2022/01/27 06:00
PMCR- 2021/01/01
CRDT- 2020/12/01 06:06
PHST- 2020/03/31 00:00 [received]
PHST- 2020/04/24 00:00 [revised]
PHST- 2020/06/08 00:00 [accepted]
PHST- 2020/12/02 06:00 [pubmed]
PHST- 2022/01/27 06:00 [medline]
PHST- 2020/12/01 06:06 [entrez]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 62.e3 [pii]
AID - 10.4111/icu.20200119 [doi]
PST - ppublish
SO  - Investig Clin Urol. 2021 Jan;62(1):90-99. doi: 10.4111/icu.20200119. Epub 2020 
      Nov 9.