PMID- 33259912
OWN - NLM
STAT- MEDLINE
DCOM- 20210812
LR  - 20210812
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 520
DP  - 2021 Jan 15
TI  - Androgens downregulate BMP2 impairing the inductive role of dermal papilla cells 
      on hair follicle stem cells differentiation.
PG  - 111096
LID - S0303-7207(20)30398-1 [pii]
LID - 10.1016/j.mce.2020.111096 [doi]
AB  - Hair follicle cyclical regeneration is regulated by epithelial-mesenchymal 
      interactions. During androgenetic alopecia (AGA), hair follicle stem cells (HFSC) 
      differentiation is impaired by deregulation of dermal papilla cells (DPC) 
      secreted factors. We analyzed androgen influence on BMPs expression in DPC and 
      their effect on HFSC differentiation to hair lineage. Androgens downregulated 
      BMP2 and BMP4 in DPC spheroids. Addition of BMP2 restored alkaline phosphatase 
      activity, marker of hair-inductivity in DPC, and DPC-induced HFSC 
      differentiation, both inhibited by androgens. Concomitantly, in differentiating 
      HFSC, an upregulation of BMPRIa and BMPRII receptors and nuclear beta-catenin 
      accumulation, indicative of Wnt/beta-catenin pathway activation, were detected. Our 
      results present BMP2 as an androgen-downregulated paracrine factor that 
      contributes to DPC inductivity and favors DPC-induced HFSC differentiation to 
      hair lineage, possibly through a crosstalk with Wnt/beta-catenin pathway. A 
      comprehensive understanding of androgen-deregulated DPC factors and their effects 
      on differentiating HFSC would help to improve treatments for AGA.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Ceruti, Julieta Maria
AU  - Ceruti JM
AD  - Instituto de Ciencia y Tecnologia Dr. Cesar Milstein - (Consejo Nacional de 
      Investigaciones Cientificas y Tecnicas CONICET- Fundacion Pablo Cassara), 
      Saladillo 2468, Ciudad de Buenos Aires, C1440FFX, Argentina.
FAU - Oppenheimer, Florencia Maia
AU  - Oppenheimer FM
AD  - Instituto de Ciencia y Tecnologia Dr. Cesar Milstein - (Consejo Nacional de 
      Investigaciones Cientificas y Tecnicas CONICET- Fundacion Pablo Cassara), 
      Saladillo 2468, Ciudad de Buenos Aires, C1440FFX, Argentina.
FAU - Leiros, Gustavo Jose
AU  - Leiros GJ
AD  - Instituto de Ciencia y Tecnologia Dr. Cesar Milstein - (Consejo Nacional de 
      Investigaciones Cientificas y Tecnicas CONICET- Fundacion Pablo Cassara), 
      Saladillo 2468, Ciudad de Buenos Aires, C1440FFX, Argentina.
FAU - Balana, Maria Eugenia
AU  - Balana ME
AD  - Instituto de Ciencia y Tecnologia Dr. Cesar Milstein - (Consejo Nacional de 
      Investigaciones Cientificas y Tecnicas CONICET- Fundacion Pablo Cassara), 
      Saladillo 2468, Ciudad de Buenos Aires, C1440FFX, Argentina. Electronic address: 
      mbalana@fundacioncassara.org.ar.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201128
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Androgens)
RN  - 0 (Biomarkers)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Bone Morphogenetic Protein 4)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Ligands)
RN  - 0 (beta Catenin)
RN  - EC 2.7.11.30 (Bone Morphogenetic Protein Receptors)
SB  - IM
MH  - Androgens/*pharmacology
MH  - Biomarkers/metabolism
MH  - Bone Morphogenetic Protein 2/*genetics/metabolism
MH  - Bone Morphogenetic Protein 4/metabolism
MH  - Bone Morphogenetic Protein Receptors/metabolism
MH  - *Cell Differentiation/drug effects
MH  - Cell Line
MH  - Cell Nucleus/drug effects/metabolism
MH  - Culture Media, Conditioned/pharmacology
MH  - Dermis/*cytology
MH  - Down-Regulation/drug effects/*genetics
MH  - Hair Follicle/*cytology
MH  - Humans
MH  - Ligands
MH  - Protein Transport/drug effects
MH  - Spheroids, Cellular/cytology/drug effects
MH  - Stem Cells/cytology/drug effects
MH  - Wnt Signaling Pathway/drug effects
MH  - beta Catenin/metabolism
OTO - NOTNLM
OT  - Androgenetic alopecia
OT  - BMP
OT  - Dermal papilla
OT  - Epithelial-mesenchymal interactions
OT  - WNT
EDAT- 2020/12/02 06:00
MHDA- 2021/08/13 06:00
CRDT- 2020/12/01 20:09
PHST- 2020/09/26 00:00 [received]
PHST- 2020/11/24 00:00 [revised]
PHST- 2020/11/24 00:00 [accepted]
PHST- 2020/12/02 06:00 [pubmed]
PHST- 2021/08/13 06:00 [medline]
PHST- 2020/12/01 20:09 [entrez]
AID - S0303-7207(20)30398-1 [pii]
AID - 10.1016/j.mce.2020.111096 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2021 Jan 15;520:111096. doi: 10.1016/j.mce.2020.111096. Epub 
      2020 Nov 28.