PMID- 33260042
OWN - NLM
STAT- MEDLINE
DCOM- 20211105
LR  - 20211105
IS  - 1872-7603 (Electronic)
IS  - 0165-0378 (Linking)
VI  - 143
DP  - 2021 Feb
TI  - Myxovirus resistance 1 (Mx1) gene: Molecular characterization of complete coding 
      sequence and expression profile in the endometrium of goat (Capra hircus).
PG  - 103247
LID - S0165-0378(20)30168-6 [pii]
LID - 10.1016/j.jri.2020.103247 [doi]
AB  - Myxovirus resistance 1 (Mx1) gene plays an important role in uterine receptivity 
      and conceptus development by creating a strong defense mechanism in the uterine 
      environment. However, the specific role of Mx1 gene is not yet documented in the 
      goat. Therefore, in the present study, full-length coding sequence (CDS) of the 
      Mx1 gene was amplified, sequenced and characterized through various Bioinformatic 
      tools. Temporal expression profile of Mx1 mRNA and protein was also examined by 
      quantitative real-time PCR (qPCR) and western blot, respectively, in the 
      endometrium of cyclic stage (non-pregnant), pregnancy stage I (16-24 days of 
      gestation) and pregnancy stage II (25-40 days of gestation) of caprine (cp). A 
      fragment of the cpMx1 gene, 2144 bp in length, was amplified from complementary 
      DNA (cDNA) with a 1965 bp open reading frame. Coding and deduced amino acid 
      sequences of the cpMx1 were aligned with other species and it exhibited 98.8-81.5 
      % identities with different species. On phylogenetic analysis, sheep and goat 
      were found belonging to the same clade but differing from large ruminants. The 
      cpMx1 protein possess the conserved signature motif (LPRGTGIVTR) of dynamin 
      superfamily and the tripartite guanosine-5'-triphosphate (GTP) binding motif 
      (GDQSSGKS, DLPG, TKPD) at the N-terminal end, and the leucine zipper motifs at 
      the C-terminal end. Both cpMx1 mRNA and protein were found to be expressed 
      maximally (P < 0.05) in the pregnancy stage I as compared to cyclic stage. It was 
      concluded that the cpMx1 gene shares major structural and probably functional 
      similarities with other species.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Jain, Asit
AU  - Jain A
AD  - College of Veterinary Science and Animal Husbandry, CGKV, Anjora, Durg, 
      Chhattisgarh, India.
FAU - Jain, Tripti
AU  - Jain T
AD  - Animal Biotechnology Centre, CGKV, Durg, Chhattisgarh, India.
FAU - Chandrakar, Khushboo
AU  - Chandrakar K
AD  - Currently, Veterinary Assistant Surgeon, Mahasamund, Chhattisgarh, India.
FAU - Singh, Mohan
AU  - Singh M
AD  - College of Veterinary Science and Animal Husbandry, CGKV, Anjora, Durg, 
      Chhattisgarh, India.
FAU - Shakya, Sanjay
AU  - Shakya S
AD  - College of Veterinary Science and Animal Husbandry, CGKV, Anjora, Durg, 
      Chhattisgarh, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201118
PL  - Ireland
TA  - J Reprod Immunol
JT  - Journal of reproductive immunology
JID - 8001906
RN  - 0 (Myxovirus Resistance Proteins)
SB  - IM
MH  - Amino Acid Motifs/genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cloning, Molecular
MH  - Conserved Sequence/genetics
MH  - Endometrium/*metabolism
MH  - Female
MH  - Goats/*genetics/metabolism
MH  - Myxovirus Resistance Proteins/*genetics/metabolism
MH  - Phylogeny
MH  - Pregnancy
MH  - Sheep/genetics/metabolism
OTO - NOTNLM
OT  - Embryo survival
OT  - Endometrium
OT  - Goat
OT  - Mx1
OT  - Myxovirus resistance
EDAT- 2020/12/02 06:00
MHDA- 2021/11/06 06:00
CRDT- 2020/12/01 20:11
PHST- 2020/07/16 00:00 [received]
PHST- 2020/10/10 00:00 [revised]
PHST- 2020/11/06 00:00 [accepted]
PHST- 2020/12/02 06:00 [pubmed]
PHST- 2021/11/06 06:00 [medline]
PHST- 2020/12/01 20:11 [entrez]
AID - S0165-0378(20)30168-6 [pii]
AID - 10.1016/j.jri.2020.103247 [doi]
PST - ppublish
SO  - J Reprod Immunol. 2021 Feb;143:103247. doi: 10.1016/j.jri.2020.103247. Epub 2020 
      Nov 18.