PMID- 33260893
OWN - NLM
STAT- MEDLINE
DCOM- 20210304
LR  - 20210304
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 23
DP  - 2020 Nov 27
TI  - Eosinophil microRNAs Play a Regulatory Role in Allergic Diseases Included in the 
      Atopic March.
LID - 10.3390/ijms21239011 [doi]
LID - 9011
AB  - (1) Background: The atopic march is defined by the increased prevalence of 
      allergic diseases after atopic dermatitis onset. In fact, atopic dermatitis is 
      believed to play an important role in allergen sensitization via the damaged skin 
      barrier, leading to allergic diseases such as allergic asthma and allergic 
      rhinitis. The eosinophil, a pro-inflammatory cell that contributes to epithelial 
      damage, is one of the various cells recruited in the inflammatory reactions 
      characterizing these diseases. Few studies were conducted on the transcriptome of 
      this cell type and even less on their specific microRNA (miRNA) profile, which 
      could modulate pathogenesis of allergic diseases and clinical manifestations 
      post-transcriptionally. Actually, their implication in allergic diseases is not 
      fully understood, but they are believed to play a role in inflammation-related 
      patterns and epithelial cell proliferation. (2) Methods: Next-generation 
      sequencing was performed on RNA samples from eosinophils of individuals with 
      atopic dermatitis, atopy, allergic rhinitis and asthma to obtain differential 
      counts of primary miRNA (pri-miRNA); these were also analyzed for asthma-related 
      phenotypes such as forced expiratory volume in one second (FEV(1)), 
      immunoglobulin E (IgE) and provocative concentration of methacholine inducing a 
      20% fall in forced expiratory volume in 1 s (PC(20)) levels, as well as FEV(1) to 
      forced vital capacity (FEV(1)/FVC) ratio. (3) Results: Eighteen miRNAs from 
      eosinophils were identified to be significantly different between affected 
      individuals and unaffected ones. Based on counts from these miRNAs, individuals 
      were then clustered into groups using Ward's method on Euclidian distances. 
      Groups were found to be explained by asthma diagnosis, familial history of 
      respiratory diseases and allergic rhinitis as well as neutrophil counts. (4) 
      Conclusions: The 18 differential miRNA counts for the studying phenotypes allow a 
      better understanding of the epigenetic mechanisms underlying the development of 
      the allergic diseases included in the atopic march.
FAU - Belanger, Emile
AU  - Belanger E
AD  - Departement des Sciences Fondamentales, Universite du Quebec a Chicoutimi, 
      Saguenay, QC G7H 2B1, Canada.
AD  - Centre Intersectoriel en Sante Durable, Universite du Quebec a Chicoutimi, 
      Saguenay, QC G7H 2B1, Canada.
FAU - Madore, Anne-Marie
AU  - Madore AM
AD  - Departement des Sciences Fondamentales, Universite du Quebec a Chicoutimi, 
      Saguenay, QC G7H 2B1, Canada.
AD  - Centre Intersectoriel en Sante Durable, Universite du Quebec a Chicoutimi, 
      Saguenay, QC G7H 2B1, Canada.
FAU - Boucher-Lafleur, Anne-Marie
AU  - Boucher-Lafleur AM
AD  - Departement des Sciences Fondamentales, Universite du Quebec a Chicoutimi, 
      Saguenay, QC G7H 2B1, Canada.
AD  - Centre Intersectoriel en Sante Durable, Universite du Quebec a Chicoutimi, 
      Saguenay, QC G7H 2B1, Canada.
FAU - Simon, Marie-Michelle
AU  - Simon MM
AD  - Department of Human Genetics, McGill University, Montreal, QC H3A 0C7, Canada.
FAU - Kwan, Tony
AU  - Kwan T
AD  - Department of Human Genetics, McGill University, Montreal, QC H3A 0C7, Canada.
AD  - McGill University and Genome Quebec Innovation Center, Montreal, QC H3A 0G1, 
      Canada.
FAU - Pastinen, Tomi
AU  - Pastinen T
AD  - Department of Human Genetics, McGill University, Montreal, QC H3A 0C7, Canada.
AD  - McGill University and Genome Quebec Innovation Center, Montreal, QC H3A 0G1, 
      Canada.
AD  - Center for Pediatric Genomic Medicine, Kansas City, MO 64108, USA.
FAU - Laprise, Catherine
AU  - Laprise C
AUID- ORCID: 0000-0001-5526-9945
AD  - Departement des Sciences Fondamentales, Universite du Quebec a Chicoutimi, 
      Saguenay, QC G7H 2B1, Canada.
AD  - Centre Intersectoriel en Sante Durable, Universite du Quebec a Chicoutimi, 
      Saguenay, QC G7H 2B1, Canada.
LA  - eng
GR  - 133605/CAPMC/CIHR/Canada
GR  - 230552/Canada Research Chairs/
PT  - Journal Article
DEP - 20201127
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cluster Analysis
MH  - Dermatitis, Atopic/*genetics
MH  - Eosinophils/*metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - Hypersensitivity/*genetics
MH  - Male
MH  - MicroRNAs/genetics/*metabolism
MH  - Middle Aged
MH  - Phenotype
MH  - Young Adult
PMC - PMC7730597
OTO - NOTNLM
OT  - allergy
OT  - asthma
OT  - atopic march
OT  - eosinophils
OT  - gene expression
OT  - miRNAs
OT  - microRNAs
OT  - sequencing
COIS- The authors declare no conflict of interest.
EDAT- 2020/12/03 06:00
MHDA- 2021/03/05 06:00
PMCR- 2020/12/01
CRDT- 2020/12/02 01:03
PHST- 2020/10/07 00:00 [received]
PHST- 2020/11/23 00:00 [revised]
PHST- 2020/11/25 00:00 [accepted]
PHST- 2020/12/02 01:03 [entrez]
PHST- 2020/12/03 06:00 [pubmed]
PHST- 2021/03/05 06:00 [medline]
PHST- 2020/12/01 00:00 [pmc-release]
AID - ijms21239011 [pii]
AID - ijms-21-09011 [pii]
AID - 10.3390/ijms21239011 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Nov 27;21(23):9011. doi: 10.3390/ijms21239011.