PMID- 33261017
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201226
IS  - 2079-7737 (Print)
IS  - 2079-7737 (Electronic)
IS  - 2079-7737 (Linking)
VI  - 9
IP  - 12
DP  - 2020 Nov 27
TI  - ATP-Dependent Chromatin Remodeler CHD9 Controls the Proliferation of Embryonic 
      Stem Cells in a Cell Culture Condition-Dependent Manner.
LID - 10.3390/biology9120428 [doi]
LID - 428
AB  - Emerging evidence suggests that chromodomain-helicase-DNA-binding (CHD) proteins 
      are involved in stem cell maintenance and differentiation via the coordination of 
      chromatin structure and gene expression. However, the molecular function of some 
      CHD proteins in stem cell regulation is still poorly understood. Herein, we show 
      that Chd9 knockdown (KD) in mouse embryonic stem cells (ESCs) cultured in normal 
      serum media, not in 2i-leukemia inhibitory factor (LIF) media, causes rapid cell 
      proliferation. This is caused by transcriptional regulation related to the cell 
      cycle and the response to growth factors. Our analysis showed that, unlike the 
      serum cultured-Chd9 KD ESCs, the 2i-LIF-cultured-Chd9 KO ESCs displayed elevated 
      levels of critical G1 phase regulators such as p21 and p27. Consistently, the DNA 
      binding sites of CHD9 overlap with some transcription factor DNA motifs that are 
      associated with genes regulating the cell cycle and growth pathways. These 
      transcription factors include the cycle gene homology region (CHR), Arid5a, and 
      LIN54. Collectively, our results provide new insights into CHD9-mediated gene 
      transcription for controlling the cell cycle of ESCs.
FAU - Yoo, Hyunjin
AU  - Yoo H
AD  - Department of Stem Cell and Regenerative Biotechnology and Humanized Pig Center 
      (SRC), Konkuk University, Seoul 05029, Korea.
FAU - La, Hyeonwoo
AU  - La H
AD  - Department of Stem Cell and Regenerative Biotechnology and Humanized Pig Center 
      (SRC), Konkuk University, Seoul 05029, Korea.
FAU - Lee, Eun Joo
AU  - Lee EJ
AD  - Department of Stem Cell and Regenerative Biotechnology and Humanized Pig Center 
      (SRC), Konkuk University, Seoul 05029, Korea.
FAU - Choi, Hee-Jin
AU  - Choi HJ
AD  - Department of Stem Cell and Regenerative Biotechnology and Humanized Pig Center 
      (SRC), Konkuk University, Seoul 05029, Korea.
FAU - Oh, Jeongheon
AU  - Oh J
AD  - Department of Stem Cell and Regenerative Biotechnology and Humanized Pig Center 
      (SRC), Konkuk University, Seoul 05029, Korea.
FAU - Thang, Nguyen Xuan
AU  - Thang NX
AUID- ORCID: 0000-0001-8945-8592
AD  - Department of Stem Cell and Regenerative Biotechnology and Humanized Pig Center 
      (SRC), Konkuk University, Seoul 05029, Korea.
FAU - Hong, Kwonho
AU  - Hong K
AD  - Department of Stem Cell and Regenerative Biotechnology and Humanized Pig Center 
      (SRC), Konkuk University, Seoul 05029, Korea.
LA  - eng
GR  - 2018/Konkuk University/
PT  - Journal Article
DEP - 20201127
PL  - Switzerland
TA  - Biology (Basel)
JT  - Biology
JID - 101587988
PMC - PMC7760864
OTO - NOTNLM
OT  - CHD9
OT  - ES cell
OT  - cell cycle
OT  - chromatin structure
OT  - transcription
COIS- The authors declare no conflict of interest.
EDAT- 2020/12/03 06:00
MHDA- 2020/12/03 06:01
PMCR- 2020/11/27
CRDT- 2020/12/02 01:03
PHST- 2020/10/19 00:00 [received]
PHST- 2020/11/25 00:00 [revised]
PHST- 2020/11/26 00:00 [accepted]
PHST- 2020/12/02 01:03 [entrez]
PHST- 2020/12/03 06:00 [pubmed]
PHST- 2020/12/03 06:01 [medline]
PHST- 2020/11/27 00:00 [pmc-release]
AID - biology9120428 [pii]
AID - biology-09-00428 [pii]
AID - 10.3390/biology9120428 [doi]
PST - epublish
SO  - Biology (Basel). 2020 Nov 27;9(12):428. doi: 10.3390/biology9120428.