PMID- 33262657
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220418
IS  - 1179-1322 (Print)
IS  - 1179-1322 (Electronic)
IS  - 1179-1322 (Linking)
VI  - 12
DP  - 2020
TI  - Effect of miR-515-5p on Proliferation and Drug Sensitivity of Retinoblastoma 
      Cells.
PG  - 12087-12098
LID - 10.2147/CMAR.S271165 [doi]
AB  - BACKGROUND: Retinoblastoma (RB) is a common malignancy in children eyes. Aberrant 
      microRNA (miR) expression is observed in many cancer cases. miR-515-5p is 
      reported to be concerned with the course of many cancers. This study explores the 
      role of miR-515-5p in proliferation and drug sensitivity of RB cells. METHODS: 
      Human RB cell lines (WERI-RB1, SO-RB50 and Y79) and human retinal pigment 
      epithelial cell line ARPE-19 were utilized in this study. Drug-resistant cells 
      SO-RB50/VCR and SO-RB50/CBP were constructed for the following experiments. The 
      expressions of miR-515-5p and Notch1 in RB cells were detected. Notch1 was 
      significantly upregulated in RB cells while miR-515-5p was notably downregulated. 
      Then, the binding relationship between miR-515-5p and Notch1 was predicted and 
      verified. RESULTS: miR-515-5p negatively regulated Notch1 expression. In vitro 
      and in vivo experiments revealed that overexpressed miR-515-5p inhibited RB cell 
      proliferation and enhanced drug sensitivity. Functional rescue experiment 
      suggested that miR-515-5p regulated RB cell proliferation and drug sensitivity 
      via inhibiting Notch1 expression. CONCLUSION: It could be concluded that 
      overexpressed miR-515-5p suppressed proliferation and drug resistance of RB cells 
      by targeting Notch1 expression, indicating that miR-515-5p might constitute a 
      promising therapy target for RB.
CI  - (c) 2020 Yuan et al.
FAU - Yuan, Xiang Wen
AU  - Yuan XW
AD  - Department of Ophthalmology, Jinan People's Hospital, Jinan City, Shandong 
      Province 271199, People's Republic of China.
FAU - Yan, Ting Qin
AU  - Yan TQ
AD  - Department of Ophthalmology, Jinan People's Hospital, Jinan City, Shandong 
      Province 271199, People's Republic of China.
AD  - Department of Ophthalmology, Tai'an Central Hospital, Tai'an City, Shandong 
      Province 271000, People's Republic of China.
FAU - Tong, Huilin
AU  - Tong H
AUID- ORCID: 0000-0001-7686-3476
AD  - Department of Ophthalmology, Laoshan Branch of the Affifiliated Hospital of 
      Qingdao University, Qingdao, Shandong 266100, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20201124
PL  - New Zealand
TA  - Cancer Manag Res
JT  - Cancer management and research
JID - 101512700
PMC - PMC7700094
OTO - NOTNLM
OT  - Notch1
OT  - drug sensitivity
OT  - microRNA-515-5p
OT  - proliferation
OT  - retinoblastoma
COIS- All authors declare that they have no conflicts of interest for this work.
EDAT- 2020/12/03 06:00
MHDA- 2020/12/03 06:01
PMCR- 2020/11/24
CRDT- 2020/12/02 05:32
PHST- 2020/07/07 00:00 [received]
PHST- 2020/10/19 00:00 [accepted]
PHST- 2020/12/02 05:32 [entrez]
PHST- 2020/12/03 06:00 [pubmed]
PHST- 2020/12/03 06:01 [medline]
PHST- 2020/11/24 00:00 [pmc-release]
AID - 271165 [pii]
AID - 10.2147/CMAR.S271165 [doi]
PST - epublish
SO  - Cancer Manag Res. 2020 Nov 24;12:12087-12098. doi: 10.2147/CMAR.S271165. 
      eCollection 2020.