PMID- 33262769
OWN - NLM
STAT- MEDLINE
DCOM- 20210716
LR  - 20210716
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 11
DP  - 2020
TI  - Characterization of miRNAs in Extracellular Vesicles Released From Atlantic 
      Salmon Monocyte-Like and Macrophage-Like Cells.
PG  - 587931
LID - 10.3389/fimmu.2020.587931 [doi]
LID - 587931
AB  - Cell-derived extracellular vesicles (EVs) participate in cell-cell communication 
      via transfer of molecular cargo including genetic material like miRNAs. In 
      mammals, it has previously been established that EV-mediated transfer of miRNAs 
      can alter the development or function of immune cells, such as macrophages. Our 
      previous research revealed that Atlantic salmon head kidney leukocytes (HKLs) 
      change their morphology, phagocytic ability and miRNA profile from primarily 
      "monocyte-like" at Day 1 to primarily "macrophage-like" at Day 5 of culture. 
      Therefore, we aimed to characterize the miRNA cargo packaged in EVs released from 
      these two cell populations. We successfully isolated EVs from Atlantic salmon HKL 
      culture supernatants using the established Vn96 peptide-based pull-down. 
      Isolation was validated using transmission electron microscopy, nanoparticle 
      tracking analysis, and Western blotting. RNA-sequencing identified 19 
      differentially enriched (DE) miRNAs packaged in Day 1 versus Day 5 EVs. Several 
      of the highly abundant miRNAs, including those that were DE (e.g. ssa-miR-146a, 
      ssa-miR-155 and ssa-miR-731), were previously identified as DE in HKLs and are 
      associated with macrophage differentiation and immune response in other species. 
      Interestingly, the abundance relative of the miRNAs in EVs, including the most 
      abundant miRNA (ssa-miR-125b), was different than the miRNA abundance in HKLs, 
      indicating selective packaging of miRNAs in EVs. Further study of the miRNA cargo 
      in EVs derived from fish immune cells will be an important next step in 
      identifying EV biomarkers useful for evaluating immune cell function, fish 
      health, or response to disease.
CI  - Copyright (c) 2020 Smith, Wajnberg, Chacko, Woldemariam, Lacroix, Crapoulet, Ayre, 
      Lewis, Rise, Andreassen and Christian.
FAU - Smith, Nicole C
AU  - Smith NC
AD  - Department of Ocean Sciences, Memorial University, St. John's, NL, Canada.
FAU - Wajnberg, Gabriel
AU  - Wajnberg G
AD  - Atlantic Cancer Research Institute, Moncton, NB, Canada.
FAU - Chacko, Simi
AU  - Chacko S
AD  - Atlantic Cancer Research Institute, Moncton, NB, Canada.
FAU - Woldemariam, Nardos T
AU  - Woldemariam NT
AD  - Department of Life Sciences and Health, OsloMet-Oslo Metropolitan University, 
      Oslo, Norway.
FAU - Lacroix, Jacynthe
AU  - Lacroix J
AD  - Atlantic Cancer Research Institute, Moncton, NB, Canada.
FAU - Crapoulet, Nicolas
AU  - Crapoulet N
AD  - Atlantic Cancer Research Institute, Moncton, NB, Canada.
FAU - Ayre, D Craig
AU  - Ayre DC
AD  - Department of Molecular Sciences, University of Medicine and Health Sciences, 
      Basseterre, Saint Kitts and Nevis.
FAU - Lewis, Stephen M
AU  - Lewis SM
AD  - Atlantic Cancer Research Institute, Moncton, NB, Canada.
AD  - Department of Chemistry & Biochemistry, Universite de Moncton, Moncton, NB, 
      Canada.
AD  - Beatrice Hunter Cancer Research Institute, Halifax, NS, Canada.
FAU - Rise, Matthew L
AU  - Rise ML
AD  - Department of Ocean Sciences, Memorial University, St. John's, NL, Canada.
FAU - Andreassen, Rune
AU  - Andreassen R
AD  - Department of Life Sciences and Health, OsloMet-Oslo Metropolitan University, 
      Oslo, Norway.
FAU - Christian, Sherri L
AU  - Christian SL
AD  - Beatrice Hunter Cancer Research Institute, Halifax, NS, Canada.
AD  - Department of Biochemistry, Memorial University, St. John's, NL, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201111
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Extracellular Vesicles/genetics/*immunology
MH  - Head Kidney/cytology
MH  - Macrophages/*immunology
MH  - *MicroRNAs
MH  - Monocytes/*immunology
MH  - Salmo salar/*genetics/*immunology
PMC - PMC7686242
OTO - NOTNLM
OT  - Atlantic salmon
OT  - RNA-seq
OT  - RNA-sequencing
OT  - extracellular vesicles
OT  - head kidney culture
OT  - macrophage
OT  - microRNA
EDAT- 2020/12/03 06:00
MHDA- 2021/07/17 06:00
PMCR- 2020/01/01
CRDT- 2020/12/02 05:33
PHST- 2020/07/27 00:00 [received]
PHST- 2020/10/09 00:00 [accepted]
PHST- 2020/12/02 05:33 [entrez]
PHST- 2020/12/03 06:00 [pubmed]
PHST- 2021/07/17 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2020.587931 [doi]
PST - epublish
SO  - Front Immunol. 2020 Nov 11;11:587931. doi: 10.3389/fimmu.2020.587931. eCollection 
      2020.