PMID- 332637
OWN - NLM
STAT- MEDLINE
DCOM- 19771130
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 18
IP  - 1
DP  - 1977 Oct
TI  - Immunological interrelationships between cholera toxin and the heat-labile and 
      heat-stable enterotoxins of coliform bacteria.
PG  - 110-7
AB  - Cholera toxin (CT) and the heat-labile (LT) toxin of Escherichia coli are known 
      to share antigenic properties. The present study examined the immunological 
      relationship of CT and the LT and heat-stable (ST) toxins of E. coli, Klebsiella 
      pneumoniae, and Enterobacter cloacae. The neutralizing capacity of equine CT 
      antiserum and of antiserum raised in rabbits to the LT toxin of the three species 
      of coliform bacteria was evaluated by determining their capacity to inhibit the 
      action of purified CT and semipurified ultrafiltration preparations of the 
      coliform LT and ST toxins in inducing water secretion as assayed by the in vivo 
      marker perfusion technique in the rat jejunum. One milliliter of antiserum to CT 
      and to E. coli and Klebsiella LT completely neutralized the secretory action of 
      each of these three toxins; effective serial dilutions of CT antiserum extended 
      to 1 to 4, whereas those of the antisera to LT were limited to 1 to 2 in most 
      instances. One milliliter of antiserum to E. cloacae LT partially neutralized 
      each of the three coliform LT toxins; serial dilutions were inactive. Antiserum 
      to E. cloacae LT did not neutralize CT. Antiserum to CT and to each of the three 
      coliform LT toxins also had a weak neutralizing effect on the ST toxins of E. 
      coli and Klebsiella, but they did not affect E. cloacae ST. Adsorption of the 
      antiserum to CT and to each of the three LT toxins by incubation with a 
      heat-inactivated preparation of either the homologous or a heterologous LT toxin 
      completely abolished the neutralizing capacity of the antisera towards both LT 
      and ST. These observations indicate that the immunological interrelationship of 
      CT and E. coli LT extends to the LT toxins of Klebsiella and E. cloacae and, 
      further, that these immunological properties are shared to a lesser extent by the 
      ST toxins of E. coli and Klebsiella.
FAU - Klipstein, F A
AU  - Klipstein FA
FAU - Engert, R F
AU  - Engert RF
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 9012-63-9 (Cholera Toxin)
SB  - IM
MH  - Antigen-Antibody Reactions
MH  - Bacterial Toxins/*immunology
MH  - Biological Assay
MH  - Cholera Toxin/*immunology
MH  - Cross Reactions
MH  - Enterobacter/*immunology
MH  - Enterobacteriaceae/*immunology
MH  - Enterotoxins/*immunology/pharmacology
MH  - Escherichia coli/*immunology
MH  - Hot Temperature
MH  - Jejunum/drug effects
MH  - Klebsiella pneumoniae/*immunology
MH  - Water-Electrolyte Balance/drug effects
PMC - PMC421201
EDAT- 1977/10/01 00:00
MHDA- 1977/10/01 00:01
PMCR- 1977/10/01
CRDT- 1977/10/01 00:00
PHST- 1977/10/01 00:00 [pubmed]
PHST- 1977/10/01 00:01 [medline]
PHST- 1977/10/01 00:00 [entrez]
PHST- 1977/10/01 00:00 [pmc-release]
AID - 10.1128/iai.18.1.110-117.1977 [doi]
PST - ppublish
SO  - Infect Immun. 1977 Oct;18(1):110-7. doi: 10.1128/iai.18.1.110-117.1977.