PMID- 33268288
OWN - NLM
STAT- MEDLINE
DCOM- 20211221
LR  - 20211221
IS  - 2452-3186 (Electronic)
IS  - 2452-3186 (Linking)
VI  - 69
IP  - 1
DP  - 2021 Jan
TI  - Metformin decreased myocardial fibrosis and apoptosis in hyperhomocysteinemia 
      -induced cardiac hypertrophy.
PG  - 103270
LID - S2452-3186(20)30059-3 [pii]
LID - 10.1016/j.retram.2020.103270 [doi]
AB  - BACKGROUND: Hyperhomocysteinemia (HHcy) is one of the major risk factors of 
      cardiovascular diseases. Metformin acts as a cardioprotective role in several 
      cardiovascular diseases, including ischemia/reperfusion, atherosclerosis, and 
      myocardial infarction. However, whether metformin protects against HHcy-induced 
      cardiac hypertrophy is unclear. METHODS AND RESULTS: HHcy model was established 
      in C57BL/6 mice with high L-methionine (L-MET) diet for 12 weeks. AC16 cells were 
      exposed to homocysteine (Hcy) and then intervened with different concentrations 
      of metformin in in vitro studies. The results showed that HHcy was able to induce 
      cardiac hypertrophy, and metformin could abrogate this effect. HHcy increased the 
      fibrosis area and induced apoptosis in the myocardium, whereas metformin could 
      reverse the detrimental effects above. TUNEL assay showed that metformin was able 
      to decrease Hcy-induced apoptosis in AC16 cells. Moreover, western blotting assay 
      revealed that metformin could decrease Hcy-induced expression of Bax and cleaved 
      caspase3, and increase the expression of Bcl-2. CONCLUSIONS: This study 
      demonstrates that metformin is able to attenuate HHcy-induced cardiac hypertrophy 
      by decreasing myocardial fibrosis and apoptosis.
CI  - Copyright (c) 2020 Elsevier Masson SAS. All rights reserved.
FAU - Zhao, Qinshuo
AU  - Zhao Q
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, 238 Jiefang Road, 
      Wuhan, 430060, China.
FAU - Song, Wei
AU  - Song W
AD  - Department of Pharmacy, Renmin Hospital of Wuhan University, 238 Jiefang Road, 
      Wuhan, 430060, China.
FAU - Huang, Jingqiu
AU  - Huang J
AD  - Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China.
FAU - Wang, Dong
AU  - Wang D
AD  - Department of Cardiology, Wuhan No.1 Hospital, Huazhong University of Science and 
      Technology, 215 Zhongshan Avenue, Wuhan, 430022, China.
FAU - Xu, Changwu
AU  - Xu C
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, 238 Jiefang Road, 
      Wuhan, 430060, China. Electronic address: xuchangwu@whu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201023
PL  - France
TA  - Curr Res Transl Med
JT  - Current research in translational medicine
JID - 101681234
RN  - 9100L32L2N (Metformin)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - *Cardiomegaly/drug therapy/etiology/pathology
MH  - Cells, Cultured
MH  - Fibrosis
MH  - Heart/drug effects
MH  - Humans
MH  - *Hyperhomocysteinemia/complications/drug therapy/pathology
MH  - Male
MH  - Metformin/*pharmacology/therapeutic use
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Myocardium/*pathology
OTO - NOTNLM
OT  - Apoptosis
OT  - Cardiac hypertrophy
OT  - Homocysteine
OT  - Metformin
OT  - Myocardial fibrosis
EDAT- 2020/12/04 06:00
MHDA- 2021/12/22 06:00
CRDT- 2020/12/03 05:32
PHST- 2020/02/11 00:00 [received]
PHST- 2020/08/23 00:00 [revised]
PHST- 2020/10/04 00:00 [accepted]
PHST- 2020/12/04 06:00 [pubmed]
PHST- 2021/12/22 06:00 [medline]
PHST- 2020/12/03 05:32 [entrez]
AID - S2452-3186(20)30059-3 [pii]
AID - 10.1016/j.retram.2020.103270 [doi]
PST - ppublish
SO  - Curr Res Transl Med. 2021 Jan;69(1):103270. doi: 10.1016/j.retram.2020.103270. 
      Epub 2020 Oct 23.